Beckman Coulter TQ-Prep, Справочник

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PN 4237395AA
3-2
OPERATION PRINCIPLES
TYPES OF PRIMING
3. After you press , the carousel is indexed sequentially until tube position 1 is
located at the sample processing position under the dispensing head. The carousel is
then in the tube loading position, Figure 3.1.
Note: If you pressed , the carousel indexes when the run cycle starts after the
preincubation.
4. The tube lifter pushes the test tube up from its slot in the carousel and into the
dispensing head.
5. The tube detector senses the presence of a test tube and continues the processing.
Note: If no tube is detected, the carousel moves to the next position and step 4 occurs. If
the tube detector detects a second consecutive empty slot in the carousel, sample
processing ends for this carousel.
6. The vortex mixer premixes the sample for 2 seconds before the first reagent is added.
7. The vortex mixer stops mixing and an electronically controlled syringe adds 600 µL of
ImmunoPrep Reagent A to the sample. At the completion of the reagent dispensing, the
vortex mixer mixes the sample for 8 seconds.
8. The vortex mixer stops mixing and an electronically controlled syringe adds 265 µL of
ImmunoPrep Reagent B to the sample. At the completion of the reagent dispensing, the
vortex mixer mixes the sample for 10 seconds.
9. The vortex mixer stops mixing and an electronically controlled syringe adds 100 µL of
ImmunoPrep Reagent C to the sample. At the completion of the reagent dispensing, the
vortex mixer mixes the sample for 10 seconds.
10. In systems with the LALS OPTION, if you selected the OPTION reagent (4 reagent)
mode, the vortex mixer stops mixing and an electronically controlled syringe adds
500 µL of the OPTION reagent to the sample. At the completion of the reagent
dispensing, the vortex mixer mixes the sample for 8 seconds.
11. The tube lifter lowers, returning the test tube to its original position in the carousel.
12. The carousel indexes to the next position and repeats the process for up to 32 sample
tubes. Any empty tube slot is ignored unless two consecutive empty slots are detected.
13. The cycle is complete when the carousel has completed one full revolution or two
consecutive empty slots are detected.
3.3 TYPES OF PRIMING
User-Requested
r
This prime dispenses 100 µL of each reagent through the dispensing tubing.
r
Use it during troubleshooting to clear air bubbles from the dispensing tubing.
r
Press to request this prime.