Beckman Coulter DxC 600i, Руководство по обучению

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UniCel DxC 600i In-Lab Training Manual A24574-AD Page 9
Professional Development Department October 2010
Sample Processing Summary
CTA Processing Summary
Programming functions are performed, and then the sample is loaded
onto the DxC 600i load area. The operator presses the green RUN
button to begin the test process. Once the RUN button is pressed, the
CTA identifies sample programming and loads the sample on the CTA
sample wheel.
If Access testing is required:
The sample cap is pierced (when a cap is present on the
sample) and the aliquot probe aspirates the sample and
delivers it to an aliquot vessel (AV). The cap piercing probe and
aliquot probe are cleaned with wash buffer.
The Pick and Place transfers each AV containing an aliquot to
an automation rack on the Access sample carousel.
The sample rack containing the sample is then transferred to
the DxC analyzer.
If no Access testing is required:
The CTA sends the sample rack directly to the DxC analyzer.
Access Processing Summary
Sample and reagent are transferred by the main pipettor from their
respective carousels to an RV in the analytical module and mixed
The RV containing the sample and reagent is transferred to the
incubator belt and incubated. The RV is transferred to the wash/read
carousel when incubation is complete.
The RV is washed three times to remove all unbound material.
Substrate is added, and the RV is incubated while being moved to the
luminometer. The luminometer reads the amount of light generated by
the chemiluminescent reaction, and the light generated is converted to
concentration.
The used RV is transferred back to the incubator belt and into the RV
waste bag.