Beckman Coulter Allegra 21 Series Скачать руководство пользователя страница 40

Operation

3-6

When run parameters for a rotor have been entered, as described below, 
they will be retained in the centrifuge memory and can be recalled by 
simply entering the rotor number. The recalled program can then be used 
for the current run or can be altered as required. 

Selecting a Rotor Number

Each rotor that can be run in the centrifuge has a rotor number; the number 
is engraved on the rotor (and on the lid of fixed angle rotors). The centri-
fuge internal memory contains a list of all rotors that can be run in the 
centrifuge, together with acceptable run parameters for each rotor. If you 
enter an unauthorized rotor number then press the [

START

] key, an error 

message will be displayed and the run will be aborted shortly after the rotor 
starts spinning.

1. Press the [

ROTOR

] key.

A rotor number (the last rotor previously centrifuged) will appear on 
the SPEED display.

2. Press the 

 or 

 cursor key until the number of the rotor in use is 

displayed.

3. Press [

ENTER

].

Nominal parameter values (time, temperature, speed, acceleration and 
deceleration curves) for the selected rotor will be displayed. You can 
use these parameters or set new values for the run.

Setting Run Speed

Centrifuge speed can be set for up to the maximum rated speed of the 
selected rotor. Either revolutions per minute (rpm) or relative centrifugal 
field (RCF) can be used to select speed. During centrifugation, the SPEED 
display indicates the actual run speed of the rotor.

Содержание Allegra 21 Series

Страница 1: ...IM 8 Allegra 21 Series GS 15 Series and Spinchron 15 Series Centrifuges Instruction Manual IMBALANCE ENTER PULSE ACCEL DECEL TEMP RPM RCF ROTOR TIME STOP START FAST STOP OPEN DOOR SPEED TIME TEMP C AC...

Страница 2: ...lter les documents joints Atenci n consulte los documentos adjuntos Attenzione consultare le informazioni allegate On power Ein Netzverbindung Marche mise sous tension Encendido Acceso sotto tensione...

Страница 3: ...ich involve the risk of electric shock or personal injury Make sure that the power switch is turned off and the instrument is disconnected from the main power source and refer such servicing to qualif...

Страница 4: ...eration may involve the use of solutions and test samples that are pathogenic toxic or radioactive Such materials should not be used in this instrument however unless all necessary safety precautions...

Страница 5: ...legra 21 Series GS 15 Series and Spinchron 15 Series Centrifuges Instruction Manual IMBALANCE ENTER PULSE ACCEL DECEL TEMP RPM RCF ROTOR TIME STOP START FAST STOP OPEN DOOR SPEED TIME TEMP C ACC DEC 2...

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Страница 7: ...xii Available Rotors xiii SECTION 1 DESCRIPTION Instrument Function and Safety Features 1 1 Instrument Function 1 1 Models 1 2 Safety Features 1 3 Name Rating Plate 1 3 Chassis 1 4 Housing 1 4 Door 1...

Страница 8: ...SECTION 4 TROUBLESHOOTING User Messages 4 1 Other Possible Problems 4 3 Emergency Access 4 3 SECTION 5 CARE AND MAINTENANCE Maintenance 5 2 Preventive Maintenance 5 2 Replacing the Rotor Chamber Gask...

Страница 9: ...e 2 1 Dimensions of the Nonrefrigerated and Refrigerated Centrifuges 2 3 Figure 3 1 Tapered Sleeve Position 3 3 Figure 3 2 Installing a Rotor 3 4 Figure 3 3 Default Parameters 3 5 Figure 4 1 Emergency...

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Страница 11: ...reverse side of the cover page SCOPE OF MANUAL This manual is designed to familiarize you with the Beckman Coulter Allegra 21 series GS 15 series and Spinchron 15 series benchtop centri fuges their f...

Страница 12: ...the centrifuge or performing instrument maintenance NOTE If the centrifuge is used in a manner other than spec ified in this manual the safety and performance of this equipment could be impaired Furth...

Страница 13: ...tage or risk of electric shock Turn the power switch off and disconnect the equipment from the main power source Refer servicing of all areas displaying either symbol to a service personnel TYPOGRAPHI...

Страница 14: ...nterference regu lations of the Canadian Department of Communications Le pr sent appareil num rique n met pas de bruits radio lectriques d passant les limites applicables aux appareils num riques de C...

Страница 15: ...5 C Humidity restrictions 95 noncondensing Dimensions Width 42 cm 16 6 in Depth 49 cm 19 3 in Height door closed 38 cm 15 0 in Height door open 80 cm 31 5 in Weight 50 kg 110 3 lb Clearances sides and...

Страница 16: ...ation profiles Deceleration 10 deceleration profiles Dimensions Width 42 cm 16 6 in Depth 64 cm 25 2 in Height door closed 38 cm 15 0 in Height door open 80 cm 31 5 in Weight 70 kg 154 4 lb Clearances...

Страница 17: ...014 F0850 Fixed Angle 25 Angle 10 000 10 730 9 000 8 690 8 50 346640 GS TB 003 F0685 Fixed Angle 25 Angle 8 000 7 010 8 000 7 010 6 85 364650 GS TB 008 F1010 Fixed Angle 35 Angle 15 300 19 890 14 500...

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Страница 19: ...ron 15 series compact benchtop centrifuges generate centrifugal forces required for a wide variety of applications Together with any of several Beckman Coulter rotors designed specifically for use in...

Страница 20: ...gerated models also have temperature control systems User messages alert the operator to conditions that may need attention MODELS The centrifuge is available in both nonrefrigerated and refrigerated...

Страница 21: ...r monitors the rotor during the run causing auto matic shutdown if rotor loads are severely out of balance At low speeds an incorrectly loaded rotor can cause imbalance Rotor insta bility can also occ...

Страница 22: ...nding the drive shaft thermistor and rotor detector are visible in the chamber bottom A gasket system around the chamber opening assures sealing Instrument gaskets have not been designed as bioseals f...

Страница 23: ...n the rotor chamber continuously monitors chamber tempera ture The micro processor calculates the required chamber temperature to maintain the selected rotor temperature NOTE In the unlikely event of...

Страница 24: ...ays The panel also contains an IMBALANCE light that flashes if rotor loads are severely out of balance Figure 1 4 The Control Panels Power Switch SPEED TIME ACC DEC IMBALANCE ENTER PULSE ACCEL DECEL R...

Страница 25: ...T STOP Pressing the FAST STOP key causes the centrifuge to decelerate to a complete stop at the maximum rate The deceleration cannot be inter rupted the centrifuge can only be restarted after the roto...

Страница 26: ...arameters ENTER Parameter speed time temperature and acceleration or deceleration curve changes made while a run is in progress must be verified by pressing the ENTER key RPM When the RPM key is press...

Страница 27: ...d Time is not counted down and the run will continue until the STOP or FAST STOP key is pressed TEMP refrigerated models only The TEMP key is used to select run temperature on refrigerated model centr...

Страница 28: ...te that data can be entered The actual real time operating conditions of the centrifuge are displayed during the run after START is pressed NOTE Error messages see Section 4 also appear on the display...

Страница 29: ...e infinity symbol indicating continuous operation lights up and the TIME display shows time elapsed since the run start After 9 hours and 59 minutes the timer will reset to 0 and continue counting ela...

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Страница 31: ...nother person INSTALLING THE INSTRUMENT WARNING Do not place the centrifuge near areas containing flammable reagents or combustible fluids Vapors from these materials could enter the centrifuge air sy...

Страница 32: ...fuge Allow sufficient drying time before running the centrifuge The centrifuge is shipped with a cone shaped plastic stabilizer installed in the rotor chamber to prevent damage to the drive motor and...

Страница 33: ...ensions of the Nonrefrigerated and Refrigerated Centrifuges 42 0 16 6 7 6 3 0 80 0 31 5 38 0 15 0 64 0 25 2 cm 42 0 16 6 7 6 3 0 49 0 19 3 80 0 31 5 38 0 15 0 in Allegra 21R GS 15R or Spinchron 15R Al...

Страница 34: ...ith the voltage listed on the name rating plate affixed to the centrifuge Never use a three to two wire plug adapter Never use a two wire extension cord or a two wire non grounding type of multiple ou...

Страница 35: ...terials in this centrifuge unless all appropriate safety precautions are taken Always use the appropriate rotors and adapters Handle all infectious samples according to good laboratory practices and m...

Страница 36: ...re an experienced user of this centrifuge you can turn to the summary for a quick review of operating steps PREPARATION AND LOADING For fast temperature equilibration cool or warm the rotor to the req...

Страница 37: ...t straight down 6 Install the rotor see Figure 3 2 according to the instructions in the appropriate rotor manual Always run the rotor with a balanced load If you are using a horizontal swinging bucket...

Страница 38: ...otor straight down onto the drive shaft Tighten the tie down screw clockwise onto the drive shaft Attach the lid and tighten with the T handle wrench Lower the yoke straight down onto the drive shaft...

Страница 39: ...s for each rotor that has been centrifuged After the initial use the program parame ters of the latest previous run will be displayed when power is applied NOTE A Program Library is provided at the ba...

Страница 40: ...ized rotor number then press the START key an error message will be displayed and the run will be aborted shortly after the rotor starts spinning 1 Press the ROTOR key A rotor number the last rotor pr...

Страница 41: ...RPM key The last digit on the SPEED display 0 will flash indicating that the rpm can be entered in 100 rpm increments with the cursor keys 2 Press the or cursor key until the required rpm is displayed...

Страница 42: ...down during continuous operation instead the infinity symbol indicating continuous operation lights up and time elapsed since the run start is displayed The run will continue until the STOP or FAST S...

Страница 43: ...setting may be needed Acceleration rates are listed in Table 3 1 Table 3 1 Acceleration Deceleration Times in minutes seconds Times are approximate actual times will vary depending on rotor load Maxim...

Страница 44: ...can be entered with the cursor keys 2 Press the or cursor key until the required number is displayed STARTING A RUN The run can be started using the parameters in memory from a previous run or using...

Страница 45: ...specified time period or from a specified time period to continuous NOTE The deceleration rate cannot be changed after decel eration starts Use the program keys as described under ENTERING RUN PARAME...

Страница 46: ...to a complete stop and the door is opened and closed 2 After the rotor stops spinning and the OPEN DOOR light comes on press the OPEN DOOR key to release the door latches then open the door NOTE To p...

Страница 47: ...POWER switch to on I Open the centrifuge door press the OPEN DOOR key and lift the door up 2 Make sure that the tapered sleeve is in place at the base of the centri fuge drive shaft before installing...

Страница 48: ...time to count down to zero or end the run by pressing either the STOP key or the FAST STOP key 8 After the rotor stops spinning and the OPEN DOOR light comes on press the OPEN DOOR key to release the...

Страница 49: ...ervice representatives USER MESSAGES CAUTION If the message SEr appears on the display do not press any keys while the message is displayed Turn the centrifuge power off O and back on I to clear the m...

Страница 50: ...then back on I to reset 81 Door open detected during run Maximum deceleration to full stop After rotor comes to a complete stop shut the centrifuge door turn the power off O then back on I to reset 8...

Страница 51: ...ng with probable causes and corrective actions required Possible causes for each problem are listed in the probable order of occurrence Perform the recommended corrective action in sequence as listed...

Страница 52: ...tor spinning 2 Power not on 3 Source power failure 4 Latch stuck 1 Wait until the rotor stops 2 Plug in the power cord turn power on I 3 See EMERGENCY ACCESS below 4 See EMERGENCY ACCESS below Display...

Страница 53: ...e panel see Figure 4 1 4 Rotate the screw clockwise until you hear a click or the door pops open 5 Remove the wrench If the rotor is still spinning close the door and wait until it stops before attemp...

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Страница 55: ...y to decontaminate the centrifuge as well as any rotors and accessories before requesting service by Beckman Coulter Field Service representatives WARNING Any maintenance procedure or servicing of thi...

Страница 56: ...onge to wipe condensation out of the chamber bowl between runs to prevent chamber icing refrigerated models If chamber icing occurs defrost before use To prevent the rotor from sticking lubricate the...

Страница 57: ...thoroughly and dry completely Lubricate the drive shaft with Spinkote after cleaning Wash the bowl using a mild detergent such as Solution 555 Rinse thoroughly and dry completely Clean the centrifuge...

Страница 58: ...damage the centrifuge no guarantee of sterility or disinfection is expressed or implied When sterilization or disinfection is a concern consult your laboratory safety officer regarding proper methods...

Страница 59: ...UPPLY LIST Refer to the applicable rotor manual for materials and supplies needed for rotors NOTE Publications referenced in this manual can be obtained by calling Beckman Coulter at 1 800 742 2345 in...

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Страница 61: ...chart below as a quick reference when duplicating run conditions Refer to Section 3 of this manual for the numbers representing acceleration and deceleration ACCEL DECEL rates Program Number User Dat...

Страница 62: ...Program Library A 2 Program Number User Date Purpose Speed Time hrs min Temp C ACCEL Rate DECEL Rate RPM RCF...

Страница 63: ...olely by Beckman Coulter REPLACEMENT Any product claimed to be defective must if requested by Beckman Coulter be returned to the factory transportation charges prepaid and will be returned to Buyer wi...

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Страница 67: ...Beckman Coulter Inc 250 S Kraemer Blvd Brea California 92821 Sales and Service 1 800 742 2345 Internet www beckmancoulter com 2009 Beckman Coulter Inc All rights reserved...

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