Optic holders, filters, and mirrors
Optic holders house filters and mirrors. Your cytometer includes several blank (empty) optic holders.
To ensure data integrity, do not leave any slots empty in a detector array when you are using the
associated laser. Always use a blank optic holder.
Base configurations
Each BD FACSymphony™ A1 flow cytometer has a base cytometer configuration that corresponds to the layout
of the installed lasers and optics in your cytometer. This base configuration is set by your field service engineer.
BD FACSDiva™ cytometer configuration
Before you acquire data using BD FACSDiva™ software, you must specify a cytometer configuration. The
cytometer configuration defines which filters and mirrors are installed at each detector.
BD FACSDiva™ software provides a BD base configuration for your BD FACSymphony™ A1 flow cytometer.
Select
Cytometer > View Configuration
to create, modify, or delete custom cytometer configurations. (See the
Cytometer and Acquisition Controls chapter of the
BD FACSDiva™ Software Reference Manual
for details.)
More information
l
About configurations (page 100)
Custom configurations and baselines
Introduction
This topic describes where to find information on how to create a custom configuration and define a baseline
for a performance check.
Overview
BD Cytometer Setup and Tracking (CS&T) software is used to define the baseline performance of your
cytometer. A baseline provides a starting point for the tracking of cytometer performance. When running a
performance check, you compare the results to the baseline.
See
Optimizing cytometer settings (page 51)
. Also see the
BD Cytometer Setup and Tracking Application Guide
for information on creating custom configurations and defining a baseline.
More information
l
Running a performance check (page 55)
Chapter 3 Cytometer setup
35
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