BD FACSymphony A1 Flow, Руководство пользователя, Страница: 28 / 126

Number Component
7 Vent valve
Procedure
Note: Be careful to avoid damaging the alarm sensor line or any of the tubing when disconnecting and filling
the sheath tank.
To prepare the sheath tank:
1. Verify that the flow cytometer is in standby mode.
Press the STANDBY button on the control panel if necessary.
2. Disconnect the green air line and blue sheath fluid line from the sheath tank.
3. Disconnect the alarm line from the alarm sensor socket by pulling lightly on both ends of the plug. See the
following illustration.
2
3
1
Number Component
1 Alarm sensor socket
2 Alarm sensor line
3 Disconnect at this point
4. Depressurize the sheath tank by pulling up on the vent valve.
5. Remove the sheath tank lid.
Unscrew the clamp knob and push down to loosen, if necessary. Tilt the cap to the side to remove it from the
tank.
6. Add up to 10 L of sheath fluid, such as BD FACSFlow™ solution, to the sheath tank.
Note: 10 L will reach the interior line on the sheath tank. Do not fill the sheath tank further.
7. Replace the sheath tank lid.
8. Make sure the gasket on the inside lip of the sheath lid is seated correctly and has not slipped out of
position.
Note: If the gasket is not seated correctly, the tank will not pressurize properly.
9. Close the sheath lid and tighten the clamp knob to finger-tight.
10. Reconnect the green air line, alarm line and the blue sheath line.
Ensure that the blue sheath fluid line is not kinked.
Note: Make sure the alarm sensor socket is off the ground by looping the line through the handle of the
sheath tank.
28 BD FACSymphony™ A1 Flow Cytometer User's Guide