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Invivo

2 

Plus

 

UM-14 Version R3.0  

Page 39 

 Invivo

2 

Plus User Manual 

 

Online video user guides 

6.9

Further information and demonstrations can be found at Ruskinn Technology Limited’s YouTube 
channel; 

http://www.youtube.com/ruskinntechnology 

Video demonstrations are available for; 

•

 

Entering and exiting the workstation chamber via the Ezee Sleeves. 

•

 

Setting oxygen and carbon dioxide values. 

•

 

Using the JuLI fluorescent cell image analyser.

 

 

Содержание Invivo2 UM-014

Страница 1: ...UM 014 Invivo2 Plus User Manual Affix Serial Number Sticker Here...

Страница 2: ...rophilic environment in which to grow facultative anaerobes Optimum cell environment o Accurate temperature control from ambient 5 C to 45 C o Accurate and automated humidity control no dry spots o Pa...

Страница 3: ...the workstation 11 2 6 Workstation overview 12 3 Workstation layout 12 3 1 Component layout 12 3 2 Front view 12 3 2 1 Rear view 13 3 2 2 Left side view Standard humidifier 14 3 2 3 Left side view Ul...

Страница 4: ...ew 26 6 2 1 Vacuum Operation 27 6 2 2 Workstation Entry 29 6 2 3 Workstation Exit 32 6 3 Single Plate Entry System SPES 33 6 4 SPES overview 33 6 4 1 Using the SPES 34 6 4 2 Internal power socket 35 6...

Страница 5: ...UK users only 48 7 4 5 Replacing the mains fuses 49 7 4 6 Service requirements 50 7 5 Spare parts and accessories 50 7 6 Overview 50 7 6 1 Spare parts and cleaning agents 51 7 6 2 Accessories 52 7 6 3...

Страница 6: ...e permissible is 4 bar gauge The maximum permissible concentration of Hydrogen in the anaerobic mixed gas is 5 5 Hydrogen The mains supply voltage fluctuations must not exceed 10 of the nominal mains...

Страница 7: ...ns the symbols found on the Invivo2 Plus Figure 1 Invivo2 Plus symbols Symbol Meaning Refer to user manual Alternating current O Off I On Primary earth connection This product complies with the essent...

Страница 8: ...ollection point for the recycling of electrical and electronic equipment Date of manufacture in format YYYY MM Installation and relocation 1 3 Invivo2 Plus should only be installed or relocated by a q...

Страница 9: ...86 mm External height 725 mm External depth 714 mm Workstation chamber internal width 1050 mm Workstation chamber internal height 479 mm Workstation chamber internal depth 575 mm Interlock internal wi...

Страница 10: ...this will damage internal components of the workstation and will invalidate the warranty The gasses are connected to the ICO2N2IC Advanced Gas mixing system See UM 011 ICO2N2IC user manual for more d...

Страница 11: ...ns inside the room the workstation is located in Powering the workstation 2 6 To switch the workstation on press the power switch item 6 in Figure 15 To switch the workstation off press the power swit...

Страница 12: ...r door The Invivo2 Plus workstation is controlled via the control panel The ICO2N2IC Advanced Gas mixing system controls the gaseous composition of the internal environment See UM 011 for more informa...

Страница 13: ...vo2 Plus UM 14 Version R3 0 Page 13 Invivo2 Plus User Manual Rear view 3 2 2 Figure 5 shows the rear view of the Invivo2 Plus workstation Figure 5 Invivo2 Plus workstation rear view 1 Cooling vent out...

Страница 14: ...Plus workstation with the standard humidifier Figure 6 Invivo2 Plus workstation left side view with standard humidifier 1 Exhaust valve outlet 2 Condenser fan 3 Pressure relief tank cover pressure re...

Страница 15: ...e Invivo2 Plus workstation with the ultrasonic humidifier Figure 7 Invivo2 Plus workstation left side view with ultrasonic humidifier 1 Exhaust valve outlet 2 Condenser fan 3 Cable gland optional acce...

Страница 16: ...3 2 5 Figure 8 shows the right side view of the Invivo2 Plus workstation Figure 8 Invivo2 Plus workstation right side view 1 Serial number Rating label 2 Portable appliance test PAT test label 3 Mixe...

Страница 17: ...gas alarm on off 8 Com Cycle active 9 Interlock purge cycle selector Press to switch between the standard 3mins and economy 2minutes interlock cycles When the switch is depressed the economy cycle is...

Страница 18: ...hour automatically 3 Internal socket switch Press to switch the internal socket on off 4 Temperature controller See section 3 3 3 for more details 5 ANO2 low 6 Gas alarm Press to switch the gas alarm...

Страница 19: ...midity controller 3 3 3 Figure 11 shows the temperature ultrasonic humidity controller Figure 11 Temperature ultrasonic humidity controller 1 Not user accessible For service personnel only 2 Not user...

Страница 20: ...trol panel 3 3 4 Figure 12 shows the interlock internal door control panel Figure 12 Interlock internal door control panel 1 Inner door LED indicator Illuminates to indicate when the interlock inner d...

Страница 21: ...or close item 3 to close the interlock outer door 2 Interlock cycle start Press to start the interlock purge cycle 3 Outer door close Press and hold with outer door open close item 1 until the outer d...

Страница 22: ...ower entry panel 3 3 7 Figure 15 shows the power entry panel Figure 15 Power entry panel 1 Gas mixer umbilical connection 2 Earth Leakage Circuit Breaker ELCB 3 Nitrogen inlet 4 Primary earth stud 5 M...

Страница 23: ...oubt consult the owner s manual for any equipment that is installed in the workstation chamber Ruskinn Technology Limited accepts responsibility for damage to any equipment installed in the workstatio...

Страница 24: ...0 Page 24 Invivo2 Plus User Manual The environmental gas composition of the workstation chamber is controlled by the ICO2N2IC Advanced Gas mixing system For more information please see UM 011 ICO2N2IC...

Страница 25: ...ock outer door press button 1 in Figure 13 The interlock door will automatically slide up To close the interlock outer door press and hold buttons 1 and 3 in Figure 13 simultaneously The door will aut...

Страница 26: ...lock Open the interlock inner door by pressing the inner door button item 2 in Figure 12 and sliding the interlock inner door backwards Slide the interlock floor tray into the workstation chamber by p...

Страница 27: ...ve Port access To minimise the time it is recommended to eliminate as much external atmosphere from the sleeve as possible prior to arm entry This can be achieved by compressing the Sleeve before inse...

Страница 28: ...on much more difficult Figure 18 Arm inserted into sleeve Shown with and without sleeve 1 Glove port handle 1 Left for Left hand side glove port 2 Right for Right hand side glove port While the arm is...

Страница 29: ...nd the sleeve exerts pressure on the arm hand This take approx 15 to 20 seconds The Sleeve should pull forcefully against both the inner surface of the Glove Port interior and the users arm and hand w...

Страница 30: ...e removal of the Cap can require a reasonable amount of force This can be made easier by pushing the uppermost part of the Handle to first break the vacuum at the upper edge of the Cap seal Figure 23...

Страница 31: ...Plus UM 14 Version R3 0 Page 31 Invivo2 Plus User Manual Figure 25 Glove port cap storage location inside workstation Repeat the procedure for the other hand if both hands are entering the workstation...

Страница 32: ...ative to the Cap Drawing the handle into the Glove Port replace the cap using the location posts to orient the Cap correctly on the Glove Port Note Care should be taken to ensure that the sleeve mater...

Страница 33: ...hamber for loading materials The SPES is also known as the mailbox SPES overview 6 4 1 The SPES consists of an external hinged flap and an internal hinged flap The external flap is held in place by a...

Страница 34: ...gure 29 SPES with external hinged flap opened Push materials through the SPES internal flap into the workstation chamber This should be done as quickly as possible to minimise gas loss from the workst...

Страница 35: ...light off press the chamber light switch item 2 in Figure 9 or item 2 in Figure 10 To switch the spotlight on press and hold the spot pedal on the foot switch control panel item 3 in Figure 14 To swit...

Страница 36: ...internal environment of the workstation chamber The cable gland is suitable for cables of diameter 3 5mm to 7mm The cable gland is located on the left hand side of the workstation Figure 31 shows the...

Страница 37: ...le port Replace the outer cap Figure 33 Gas sample port To use the gas sample port Connect the independent gas meter to the gas sample port Vacuum port 6 8 4 The vacuum port is used to remove liquids...

Страница 38: ...um is no longer required remove the internal vacuum hose from the vacuum port by pressing the top of the metal part of the internal section of the vacuum port and pulling the vacuum hose The hose shou...

Страница 39: ...and demonstrations can be found at Ruskinn Technology Limited s YouTube channel http www youtube com ruskinntechnology Video demonstrations are available for Entering and exiting the workstation cham...

Страница 40: ...ximum concentration of 70 by volume propanol in distilled water Tristel Fuse Sachet 1 sachet diluted in 3 litres of distilled water or Tristel Duo Foamer Tristel Fuse Sachets and Duo Foamer are availa...

Страница 41: ...Check that the front screen seals are clean Clean the front screen seals using an approved cleaning agent if required Place the front screen onto the mounting studs Reconnect the glove port selector...

Страница 42: ...ol panel Wipe dry using paper towels Cleaning the interlock chamber The items inside the workstation chamber that require cleaning are The floor The floor tray The ceiling panel The rear wall The left...

Страница 43: ...open one of the glove port covers see section 6 2 to equalise the pressure between the workstation chamber and the external environment For standard humidity remove the pressure relief tank bung For u...

Страница 44: ...inked or overlapping Reinstall the O rings over the Ezee Sleeve Note that the O rings will be a tight fit This is intentional to provide a gas tight seal Note that the O rings must completely cover th...

Страница 45: ...ls Place the old detox sachets in the interlock chamber and bring the new detox sachets into the workstation chamber Close the interlock inner door see section 6 1 3 for more details Whilst lifting th...

Страница 46: ...ck Remove the catalyst sachet from its packaging Open the interlock outer door see section 6 1 2 for more details Place the catalyst sachet in the interlock chamber Close the interlock outer door see...

Страница 47: ...the floor tray to expose the detox sachets Whilst holding the floor tray up slide one detox sachets forwards and remove Remove the new catalyst sachet from its packaging Whilst lifting the front of th...

Страница 48: ...onnect from the mains power supply Remove the plug from the mains socket Using a small flat bladed screw driver remove the fuse cover from the mains plug Figure 37 shows the fuse removal Figure 37 Mai...

Страница 49: ...val of the mains fuse holder Figure 38 mains fuse holder removal Replace the mains fuses The fuse ratings are 240V 50Hz F3 15A H250V 220V 60Hz F5A H250V 110V 60Hz F5A H250V 100V 50 60Hz F5A H250V Fuse...

Страница 50: ...iennially 2 yearly Qualified service engineer To arrange an annual or biennial service contact your local distributor Note that the biennial service includes an annual service Note that the annual ser...

Страница 51: ...40 Invivo2 Plus end user spare parts and cleaning agents list Part Where used Small Ezee Sleeve Pair Ezee Sleeve Medium Ezee Sleeve Pair Ezee Sleeve Large Ezee Sleeve Pair Ezee Sleeve Glove port seal...

Страница 52: ...on castors Stand height approximately 800mm Stand with fixed feet Stand with castors Anti vibration table for multi well plates Holds 4 multi well plates 6 well to 96 well protecting the plates from v...

Страница 53: ...se has blown Wait for the workstation chamber to cool and the heater mat thermal protection switch will reset itself Contact your local distributor The LED indicators are not illuminated 24V power sup...

Страница 54: ...r is blocked The interlock inner door is stuck Check for obstructions to closing the interlock inner door and remove them Spillages can cause the interlock inner door to stick Clean the interlock inne...

Страница 55: ...LIED WARRANTY OF FITNESS FOR A PARTICULAR PURPOSE THE IMPLIED WARRANTY OF MERCHANTABILITY OR ANY IMPLIED WARRANTY ARISING OUT OF A COURSE OF DEALING OR OF PERFORMANCE CUSTOM OR USAGE OF TRADE Ruskinn...

Страница 56: ...t not be disposed of at a household waste site Instead it should be taken to the appropriate collection point for the recycling of electrical and electronic equipment Alternatively please contact your...

Страница 57: ...ales sales ruskinn com Technical support techsupport ruskinn com General enquiries ruskinnoffice ruskinn com Website www ruskinn com YouTube channel http www youtube com ruskinntechnology Ruskinn Tech...

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