Agilent Technologies MX3000P, Руководство по установке и эксплуатации

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Performing the Instrument Qualification Test Run
The Instrument Qualification Test verifies that the biological performance of
an instrument experimental run is within factory specifications. Completing
the run requires approximately 2.5 hours.
The qualification test run uses the installation qualification test plate and a
preconfigured plate setup and thermal profile setup. No additional software
setup steps are required. A validation protocol Wizard will provide
instructions in a series of dialog boxes before, during, and after the run.
Since the Qualification Test is a fixed test, only the actions directed by the
validation protocol Wizard can be completed.
Do not attempt to modify the Qualification Test experiment or to
analyze data during the Qualification Test run.
Note
Wear chemical-resistant powder-free gloves whenever handling
the qualification plate or other sample plates. This will ensure that
no finger oils or other debris are transferred to the optical tube
seal.
1. Prepare the installation qualification test plate just prior to starting the
run. Thaw the plate contents at room temperature for 15–30 minutes,
leaving the plate sealed in the foil wrapper while thawing.
After thawing, remove the foil wrapper and then mix the contents of the
wells by completing the following actions
five times :
•
Invert the plate. Use a sharp, swift, downward motion to move the
liquid to the seal on top of each well. Observe the liquid to verify
that the liquid has moved from the bottoms to the tops of the tubes.
•
Re-invert the plate, and use the same motion to return the liquid to
the bottom of each well, again verifying liquid movement before
proceeding.
After completing five mixing cycles, collect the liquid at the bottom of
the wells. Brief centrifugation of the plate at 1000 rpm is highly
recommended, in order to dislodge any bubbles and to collect the
liquid.
If a plate centrifuge is not available, shake the liquid down to the
bottom of the tubes (using the same motion described above for
mixing). Shake-down the plate until the majority of the liquid is at the
bottom of the tubes and only minimal amounts of liquid are retained on
the tube seal or sides. It is important to shake-down any noticeable
liquid on the tube seal (e.g. visible semi-circles or crescent shapes when
viewed from above). Small amounts of liquid spray retained on the tube
sides should not affect test results.
Mx3000P and Mx3005P QPCR Systems Setup and User’s Guide 15