Agilent Technologies Magnis NGS Prep System Скачать руководство пользователя страница 2

Before You Begin

2

Magnis NGS Prep System Quick Reference Guide

Before You Begin

Product

Description

The Magnis NGS Prep System is an automated liquid handling system for next generation 
sequencing library preparation and/or target enrichment of human nucleic acid samples.

Principle of the

Procedure

The Agilent Magnis NGS Prep System is a liquid handler that offers start-to-finish automation of 
library preparation and target enrichment protocols for next generation sequencing (NGS). The 
starting material is fragmented genomic DNA (gDNA) purified from a cell or tissue sample, blood 
sample, or formalin-fixed paraffin-embedded (FFPE) sample. The end result is a target-enriched 
DNA library ready for sequencing. 

The hardware components make up the instrument. See 

“Instrument Components” 

on page 4 for 

a list of these components.

The instrument is controlled through the software component of the system, which is displayed 
and operated through the LCD touchscreen.

Limitations of

Use

The Magnis NGS Prep System has been validated for use with Agilent Magnis NGS kits.

Materials

Provided

Safety Precautions

The Magnis NGS Prep System is designed for safe operation when it is used in the intended 
manner. Using the system for purposes other than those intended may impair those safety 
protections.

Installation

The system must be installed by an Agilent engineer or Agilent authorized service provider.

Do not attempt to manually lift the instrument. To move the instrument, use an automated 
forklift or lift table that can lift at least 100 kg.

When adjusting the placement of the instrument on the laboratory bench, take care to avoid 
pinching hazards on the instrument.

Retain the locking jigs that secure the gantry during shipment. Anytime the instrument is 
moved, secure the gantry using the jigs.

Electrical

Standard electrical safety precautions should be applied, including the following:

Per North American and IEC requirements, install the instrument in a location where branch 
circuit protection is available in the power network.

Install the instrument with the Agilent supplied power cord that is compatible with the electrical 
outlets in your region. Do not substitute with a power cord from another source.

Table 1

Materials provided with the Magnis NGS Prep System

Materials

Magnis NGS Prep System instrument and preloaded touchscreen software

Power cord

Functional test certificate

USB disk with PDFs of the User Guide and Quick Reference Guide

Содержание Magnis NGS Prep System

Страница 1: ...7 Log in to the system 7 Creating User Accounts 8 About user access levels 8 Add new user accounts 9 Decontaminating the System 9 Running Protocols 10 1 System prep 10 2 Reagent prep 11 3 Protocol se...

Страница 2: ...tem is designed for safe operation when it is used in the intended manner Using the system for purposes other than those intended may impair those safety protections Installation The system must be in...

Страница 3: ...not service the electrical components unless you are qualified to do so Fluids and Reagents Never process reagents not intended for use with the Magnis NGS Prep System Observe the relevant safety reg...

Страница 4: ...rument in high static environments ESD STM5 1 1998 Class 3B Environmental Requirements Operating temperature Keep the ambient temperature between 15 C and 25 C Operating humidity Keep the humidity lev...

Страница 5: ...eps Chiller module for reagent storage Tube holders for liquid reagents 6 total Platforms to hold tip boxes 4 Platform to hold Beads Buffers Plate Opening to tip waste bin LED indicator lights 2 Micro...

Страница 6: ...status of the instrument based on the color of the LED indicator lights that illuminate the entire plate filling area Micropipettor Barcode scanner UV tube Instrument deck LED indicator light LED indi...

Страница 7: ...the user name and password for your account into the provided fields The Agilent engineer or Agilent authorized service provider creates a user account during system installation that has advanced us...

Страница 8: ...of markers called teach points that are printed on the instrument deck Auto teaching ensures that during a run the micropipettor is accurately aligned with the tubes or wells at each deck position You...

Страница 9: ...s to send an email alert to the user it sends it to all email addresses listed in the Email address es field 8 Mark the check box labeled Email alert on run complete if you want the system to send an...

Страница 10: ...prep Prepare the Magnis NGS Prep System so that it is ready to run a protocol 1 Verify that the instrument deck is cleared of all labware from previous runs 2 Turn on the instrument and close the inst...

Страница 11: ...orms an IHC At completion of the IHC the Enter Run Info step opens 2 Complete the Run Setup steps and start the protocol run as instructed in the user manual for your Magnis Target Enrichment Kit The...

Страница 12: ...llect the final library samples i e the green library strip tube from the chiller module 3 Re seal the wells of the strip tube using a fresh foil seal strip then store at the temperature recommended i...

Страница 13: ...7 9770 option 3 4 4 Or send an email to techservices agilent com For Europe Middle East Africa and India Call 00800 345 600 toll free or 49 69 8679 7730 Or send an email to genomics_tech_europe agilen...

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