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Agilent G1978B Multimode Source for 6410 Triple Quad LC/MS
User Guide

Agilent Technologies

3
Reference

Safety and Specifications

32

Safety

32

Safety Symbols

36

Technical Specifications

37

Overview

38

Benefits of a multimode source

38

Mobile phase considerations when using the multimode source

46

Source comparison in positive ion mode

48

Source comparison in negative ion mode

49

Guidelines

50

Guidelines for multimode source settings in MM-ES mode

50

Guidelines for multimode source settings in MM-APCI mode

51

Guidelines for multimode source settings in MM-ES+APCI mode

52

Empirical Formulas for high resolution MS

53

G2421A MM-ES Calibration Ions

53

G2432A MM-APCI Calibration Ions

54

Tuning Mix and Test Mix

55

G2421A ES Tuning Mix (100mL)

55

G2432A APCI Tuning Mix (100mL)

56

Chemical structures in the multimode test mix

57

This chapter contains reference information to help you run your 6400 Series
Triple Quad LC/MS with a multimode source.

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Содержание G1978B

Страница 1: ...Agilent Technologies Agilent G1978B Multimode Source for 6410 Triple Quad LC MS User Guide...

Страница 2: ...in the sep arate agreement shall control Technology Licenses The hardware and or software described in this document are furnished under a license and may be used or copied only in accor dance with th...

Страница 3: ...your multimode ion source 1 Basic Operation and Maintenance This chapter describes basic operation and maintenance for the multimode source 2 Installation This chapter tells you how to install the mul...

Страница 4: ...4 Multimode Source for 6410 Triple Quad LC MS User Guide...

Страница 5: ...ce 23 To convert from multimode to ESI or APCI 24 To convert from ESI or APCI to the multimode source 25 3 Reference 31 Safety and Specifications 32 Safety 32 Safety Symbols 36 Technical Specification...

Страница 6: ...uad LC MS User Guide Contents G2421A MM ES Calibration Ions 53 G2432A MM APCI Calibration Ions 54 Tuning Mix and Test Mix 55 G2421A ES Tuning Mix 100mL 55 G2432A APCI Tuning Mix 100mL 56 Chemical stru...

Страница 7: ...d Maintenance To set up a method to use the multimode source 8 To open the multimode source 10 To clean the multimode source daily 11 To clean the multimode source weekly 13 To check tuning with the m...

Страница 8: ...ose an ionization mode from the Ion Mode list You may set the ionization mode to one of the following ESI APCI ESI APCI The Ion Mode selection ESI APCI will specify a method for alternating ESI and AP...

Страница 9: ...MS diverter valve is an integral part of the G1978B safety system The LC mobile phase flow must always be connected to the diverter valve inlet filter Never bypass the diverter valve and connect direc...

Страница 10: ...esent within the chamber 3 Check that the vaporizer temperature sensor is straight and extends 15mm from back of chamber 4 Check that the separator is aligned vertically 5 Check that the APCI corona n...

Страница 11: ...Wash bottle Water reagent grade or better 1 Turn off the spray chamber 2 Remover the nebulizer and APCI corona needle 3 Remove the cosmetic cover The thermocouple probe will need to be removed if any...

Страница 12: ...shield 8 Replace the nebulizer and the APCI corona needle 9 Install the thermocouple probe and adjust it so that it protrudes 15 mm from the inner spray chamber wall See Figure 2 Figure 2 Installing...

Страница 13: ...agent grade or better 1 Do the steps in To remove the multimode source on page 23 2 Fill the spray chamber with clean mobile phase or with a mixture of isopropanol and water 3 Scrub the corona insulat...

Страница 14: ...the spray shield 9 Use abrasive paper to gently clean the spray shield Dampen a clean cloth and wipe the spray shield 10 Rinse the area around the spray shield Then wipe the area around the spray shi...

Страница 15: ...e Electrospray calibrant back into its original bottle or another suitable container rinse the calibrant bottle with acetonitrile pour the APCI calibrant into the calibrant bottle and attach the calib...

Страница 16: ...16 Multimode Source for 6410 Triple Quad LC MS User Guide 1 Basic Operation and Maintenance To check tuning with the multimode source...

Страница 17: ...18 Step 2 Install the HV control PCA and cables 19 To remove the multimode source 23 To convert from multimode to ESI or APCI 24 To convert from ESI or APCI to the multimode source 25 This chapter con...

Страница 18: ...the Multimode Enablement Kit contains the following parts Multimode Bd HV Cable p n G1960 60858 Multimode HV PCA p n G1960 61015 Multimode Bd Power Data Cable p n G1960 60873 Figure 3 From left to ri...

Страница 19: ...ve the CDS cover top side front and the Aux Module cover 3 Disconnect the ribbon cable that connects the valve PCA to the Vcap Vchamber power supply Then disconnect the Vcap and Vchamber cable from th...

Страница 20: ...of the AUX Module 7 Connect ribbon cable between the valve PCA and Vcap Vchamber power supply Figure 7 Connecting the valve PCA to the Vcap Vchamber power supply 8 Connect the Vcap and Vchamber cable...

Страница 21: ...e system and then plug it to the APCI HV connector Attach the other end to the chassis with the o ring and the nut see Figure 10 Figure 10 Connecting the APCI HV to the chassis 11 Insert the cable G19...

Страница 22: ...necting all connectors 14 Put back the side top front and CDS cover 15 Plug the system power cord back on and turn the front switch on The pump down process will start 16 Start the MassHunter Workstat...

Страница 23: ...Interlock and multimode HV cable 6 Unscrew the nebulizer gas line from the nebulizer 7 Unscrew the LC sample tubing from the nebulizer 8 Open the latch on the source and open the source 9 Remove the m...

Страница 24: ...e that the hole in the spray shield is in the 12 o clock position 3 For an APCI ion source connect the vaporizer heater cable and the APCI high voltage cable 4 For all sources reconnect the nebulizer...

Страница 25: ...ulizer gas tubing from the currently installed ion source 4 Disconnect the LC MS sample inlet tubing 5 If the APCI source is installed remove the APCI vaporizer heater cable and APCI high voltage cabl...

Страница 26: ...d capillary cap for ESI or APCI 8 Remove the capillary cap If needed moisten a clean cloth with isopropyl alcohol and wipe the capillary cap See Figure 14 Figure 14 Spray shield removed 9 Place the ca...

Страница 27: ...r the spray shield See Figure 16 Figure 16 Multimode spray shield installed 12 Remove the shipping cover from the multimode source spray chamber Field shaping electrode 9 o clock position Field shapin...

Страница 28: ...allation To convert from ESI or APCI to the multimode source Figure 17 Multimode Spray Chamber 13 Install the spray chamber on the spray chamber mount Figure 18 Multimode source with I Button 14 Insta...

Страница 29: ...to the multimode source Figure 19 No nebulizer on top of the multimode source 15 Connect the 1 8 inch nebulizer gas tubing from the LC MS mainframe to the nebulizer gas fitting See Figure 20 Figure 2...

Страница 30: ...w the steps in Step 2 Install the HV control PCA and cables on page 19 WARNING The LC MS Liquid Chromatograph diverter valve is an integral part of the G1978B safety system The LC mobile phase flow mu...

Страница 31: ...n mode 49 Guidelines 50 Guidelines for multimode source settings in MM ES mode 50 Guidelines for multimode source settings in MM APCI mode 51 Guidelines for multimode source settings in MM ES APCI mod...

Страница 32: ...e nebulizer needle tip replace the needle The vaporizer temperature sensor is very sharp and can pierce your skin Do not touch the tip especially when you analyze toxic substances or when you use toxi...

Страница 33: ...ry and capillary cap are at high voltage up to 4 kV If you connect the instrument to an ungrounded or improperly grounded power source you create a shock hazard for the operator and can damage the ins...

Страница 34: ...d as hazardous waste Dispose of used pump fluid as specified by your local regulations Environmental Conditions This equipment must be installed in an environment of Category II installation as define...

Страница 35: ...at come in contact with the sample path Oil from your fingers is difficult to remove When you set parts down place them on clean lint free cloths or clean aluminum foil not directly on the laboratory...

Страница 36: ...placed on the product within the area where hazardous voltage is present or shock hazard can occur Only trained service persons should perform work in this area WARNING This symbol is placed on the pr...

Страница 37: ...Operating Temperature 15 C to 35 C 59 F to 95 F Analytical specifications will be met only within a temperature range of 21 C 3 C 70 F 6 F Operating Humidity 15 to 95 non condensing at 35 C Operating...

Страница 38: ...low rates 50 to 2000 L min The multimode source can be run in three different modes of operation MM ES MM APCI or MM ES APCI Why use the multimode source A single ion source with a single nebulizer pe...

Страница 39: ...neously This ability increases the effective duty cycle of the mass spectrometer Another key feature is the much greater operational flow rate that is available in this design Mixed mode operation is...

Страница 40: ...urce operational description Figure 22 Graphical representation of multimode source 1 Liquid enters the grounded nebulizer 2 A charged aerosol is made in the ESI Zone 3 The aerosol is dried by IR lamp...

Страница 41: ...ES Zone Figure 23 Graphical representation of MM ES zone 1 Liquid enters the grounded nebulizer 2 The charging electrode charges the liquid 3 Nebulizing gas pushes the charged aerosol past the chargin...

Страница 42: ...ode source Infrared Drying Figure 24 Graphical representation of infrared drying 1 The charged aerosol passes the reversing electrode and enters the thermal container 2 The thermal container confines...

Страница 43: ...phical representation of MM APCI zone 1 Neutral analytes and MM ES charged analytes pass through the MM APCI Zone 2 A corona is formed between the APCI corona needle and the pin 3 Neutral analytes in...

Страница 44: ...4 Multimode Source for 6410 Triple Quad LC MS User Guide 3 Reference Benefits of a multimode source Multimode source side view Figure 26 Graphical representation of a side view of the multimode source...

Страница 45: ...ltimode Source for 6410 Triple Quad LC MS User Guide 45 Reference 3 Benefits of a multimode source Multimode source top down view Figure 27 Graphical representation of a top view of the multimode sour...

Страница 46: ...tely one fifth that of prior recommendations The need for lower electrolyte concentrations is due to the physics of ESI ion formation and charge separation in the multimode source The following are ex...

Страница 47: ...n linked with a reduction in response of the analytes For electrolytes added to the mobile phase used for mixed mode operation use care in selecting the anion as it may affect the response in negative...

Страница 48: ...uide 3 Reference Source comparison in positive ion mode Source comparison in positive ion mode Using the multimode source in MM ES APCI mode with positive polarity you can acquire a single data file c...

Страница 49: ...mparison in negative ion mode Source comparison in negative ion mode Using the multimode source in MM ES APCI mode with negative polarity you can acquire both hexanesulfonic acid and dinitrobenzotrifl...

Страница 50: ...set the drying gas temperature to vapor temperature or lower If running the vaporizer hot 175 C then set the drying gas temperature to 350 C Remember you want the vaporizer to control the drying and t...

Страница 51: ...solvent composition Corona current Set to 5 A positive or negative The corona voltage will be 4000 V at 6 A Vcap Set to 2000 V The APCI response is usually better at the higher end of the Vcap range C...

Страница 52: ...ks better for MM APCI MM ESI is usually unaffected except for sodium adduction The vaporizer will compensate for flow rate or solvent composition Capillary Voltage Set to 2000 V You are balancing ESI...

Страница 53: ...1 971900 C30 H19 O6 N3 P3 F48 fragment 1807 937049 C35 H17 O6 N3 P3 F60 HP 2121 H 2121 933500 C42 H19 O6 N3 P3 F72 fragment 2307 905049 C45 H17 O6 N3 P3 F80 HP 2721 H 2721 895100 C54 H19 O6 N3 P3 F96...

Страница 54: ...H19 O6 N3 P3 F12 HP 0921 H 922 010300 C18 H19 O6 N3 P3 F24 fragment 1307 969049 C25 H17 O6 N3 P3 F40 HP 1521 H 1521 971900 C30 H19 O6 N3 P3 F48 fragment 1807 937049 C35 H17 O6 N3 P3 F60 HP 2121 H 212...

Страница 55: ...1 mg HP 0621 1 mg HP 0921 5 mg HP 1521 5 mg HP 2121 5 mg HP 2721 10 mg HP 0585 5 mg betaine 1 mg tfa ammonium salt 26 2 mg Trade Name Chemical Description HP 0321 Non fluorinated triazatriphosphorine...

Страница 56: ...nol 11 87 g HP 0321 1 mg HP 0621 1 mg HP 0921 5 mg HP 1521 5 mg HP 2121 5 mg purine 1 mg chloroform 1 476 g Trade Name Chemical Description HP 0321 Non fluorinated triazatriphosphorine HP 0621 Fluorin...

Страница 57: ...e Quad LC MS User Guide 57 Reference 3 Chemical structures in the multimode test mix Chemical structures in the multimode test mix The following diagram shows the chemical structure of the four compon...

Страница 58: ...58 Multimode Source for 6410 Triple Quad LC MS User Guide 3 Reference Chemical structures in the multimode test mix...

Страница 59: ...G graphic Infrared Drying 42 MM APCI Zone 43 MM ES Zone 41 side view 44 top down view 45 guideines MM ES 50 guidelines 50 APCI 51 MM ES APCI 52 I installation 17 M method basic setup 8 mobile phase 46...

Страница 60: ...60 Multimode Source for 6410 Triple Quad LC MS User Guide Index...

Страница 61: ......

Страница 62: ...USA First Edition October 2006 G1978 90010 G1978 90010 www agilent com Agilent Technologies In This Book This book contains installation operation maintenance and troubleshootinginstruction for the Mu...

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