Advance acoustic Mupid-2plus, Инструкция по эксплуатации, Страница: 6 / 15

12
6 Troubleshooting
Problem Causes and Measures
- Make sure that the electric cord is plugged into the outlet.
- Make sure that the operation button has been pressed.
- If the migration tank lid is not closed properly the safety switch
cannot go ON; make sure the lid is closed firmly.
- The fuse may have blown; contact a distributor or our service
center for repair.
- Make sure that the power supply is firmly connected to the
migration tank.
- Make sure that the type and concentration of buffer are
correct.
- Make sure that the gel is fully suffused with buffer.
- The electrode may be broken; contact a distributor for repair.
- Make sure that the voltage setting is correct.
- The type, concentration or temperature of the gel and buffer
can make a great difference.
- Make sure that buffer is not being re-used.
- Make sure there are no air bubbles in the gel.
- Agarose may not have sufficiently dissolved at the time of gel
preparation.
- Make sure that the migration tank was set up on a level
surface.
- Make sure that the gel was prepared evenly.
- Make sure that the wells have a good rectangular shape.
- Check if the concentration of stain is too low, or if it has been
re-used.
- Try extending the staining time.
- Make sure the amount of sample is sufficient.
- Make sure that the light source of the appropriate wave length
for the stain is used.
- If the gel is being observed in the migration tank or gel tray,
try observing the gel alone.
- Try testing again using a shorter migration time.
5P 12P
"Gel maker set L" is standard for Mupid-2plus. The contents of the Gel maker set L
are as follows:
Gel maker stand-L: 1 Gel tray-S: 4
Comb-L: 2 Gel tray-L: 2
5
Standard set contents
[5 [
1-2 Gel Maker Set L
Set the comb in the gel maker stand to form wells in
the gel for application of the sample. Wells all have
a thickness of 1 mm and the width and number of
wells vary as follows:
Mini gel use:
4 mm (W)
×
8 wells or 6 mm (W)
×
6 wells
Large gel use:
4 mm (W)
×
17 wells or 6 mm (W)
×
12 wells
Set the gel tray in the gel maker stand, and pour the
gel-forming solution here. There are 2 sizes and each
of them has a migration indication line.
Gel tray-S: 52 mm (W)
×
60 mm (L) [gel size]
Gel tray-L: 107 mm (W)
×
60 mm (L) [gel size]
Set the gel tray and comb
here to prepare gel.
At maximum, 4 mini gels or
2 large gels can be prepared
at once.
Comb-L
Gel tray-S
Gel tray-L Gel Maker Stand-L
Several other Mupid-related options are available.
Please consult our website (http://www.mupid.com/English/).
The indicator LED
does not light
and samples
cannot be
migrated.
The indicator LED
is lit but samples
cannot be
migrated.
Migration is slow.
Bands are
distorted.
The bands cannot
be observed.