Accu-Scope EXI-410 Series, Руководство пользователя

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EXI-410 INVERTED MICROSCOPE SERIES
ACCU-SCOPE ®
73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com 23
Operation
(continued)
Centering the Light Annulus
(Continued)
4. While observing through the centering telescope,
adjust its focus on the phase annulus
②
of the
objective and corresponding light annulus
①
. Refer to
figure on previous page.
5. Insert the 2mm hex wrench into the two centering
screw holes on the phase slider
③
. Tighten and
loosen the centering screws until the light annulus is
superimposed on the phase annulus of the objective.
6. Repeat the steps above to adjust centering with other
objectives and corresponding light annuli.
NOTES:
• Halo-like ghost images of the light annulus may
sometimes appear. It this occurs, superimpose the
brightest light annulus image over the phase
annulus.
• When a thick specimen is moved or replaced, the
light annulus and the phase annulus may deviate.
This is usually due to the amount of media or
certain wellplate inconsistences. This can reduce
image contrast. If this occurs, repeat steps 1-5 for
readjustment.
• The centering procedure may have to be repeated
in order to get the best possible contrast if a
specimen slide or the bottom surface of a culture
vessel is not flat. Center the light annulus using
objectives in the order of lower to higher
magnifications.
Emboss Contrast Observation
Emboss contrast microscopy requires a condenser-side emboss contrast slider and eyepiece-
tube-side emboss contrast slider. These were shipped with the microscope and the installation
and operation instructions are below.
Hexagonal
wrench
③
③