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6-20
CELL-DYN
®
3200 System Operator’s Manual
9140181H—October 2001
Calibration Procedures
Auto-Cal Method
Section 6
Determining Reference Values
The minimum and maximum values for each parameter, displayed above the
reference value fields, apply to both calibrator material and fresh whole blood.
Calibrator
Obtain the reference values from the assay sheet that is packaged with the
calibrator material.
Fresh Whole Blood
Follow the procedure below to determine the calibration reference values using
fresh whole blood.
NOTE:
No more than two hours should elapse between determining the
Reference Mean Values and performing the calibration.
WARNING: Potential Biohazard.
Consider all specimens and reagents,
controls, calibrators, etc., that contain human blood or serum as potentially
infectious. Wear gloves, lab coats, and safety glasses and follow other
biosafety procedures as specified in the OSHA Bloodborne Pathogen Rule
(29 CFR 1910.1030) or other equivalent biosafety procedures.
1. Go to a reference hematology instrument or use appropriate hematology
methods with one sample of normal, fresh whole blood.
NOTE:
The Auto-Cal method is designed for 1 sample. If you want to use
more than one fresh whole blood, you must use the Enter Factor
method.
2. Run a minimum of 10 replicates from this sample on the reference
instrument.
NOTE:
Because the same sample will be used to first obtain reference
values on a reference instrument then to calibrate in the Open and
Closed Modes, it is important to begin with a sufficient amount of
sample.
3. If a mean value for each parameter based on at least 10 runs is not
automatically calculated by the reference hematology instrument or
hematology methods, use a calculator to determine the Reference Mean for
each parameter. For example:
The cumulative Reference WBC Mean is 6.53 when the WBC results from
each run are as follows:
• RUN 1 = 6.6, RUN 2 = 6.4, RUN 3 = 6.7
• RUN 4 = 6.5, RUN 5 = 6.6, RUN 6 = 6.4
• RUN 7 = 6.7, RUN 8 = 6.3,
RUN 9 = 6.5
• RUN 10 = 6.6
The cumulative mean of 6.53 equals the sum of the values (65.3) divided by
the 10 runs.
4. Save these values; they will be used to calibrate the CELL-DYN 3200
System.
Содержание CELL-DYN 3200
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Страница 52: ...1 4 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Use or Function Overview Section 1 NOTES ...
Страница 132: ...3 38 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Principles of Operation References Section 3 NOTES ...
Страница 154: ...5 2 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Operating Instructions Overview Section 5 NOTES ...
Страница 234: ...5 82 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Operating Instructions Run Menu Section 5 NOTES ...
Страница 300: ...6 2 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Calibration Procedures Overview Section 6 NOTES ...
Страница 356: ...6 58 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Calibration Procedures References Section 6 NOTES ...
Страница 362: ...8 2 CELL DYN 3200 System Operator s Manual 9140181K July 2002 Hazards Overview Section 8 NOTES ...
Страница 370: ...8 10 CELL DYN 3200 System Operator s Manual 9140181K July 2002 Hazards References Section 8 NOTES ...
Страница 372: ...9 2 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Service and Maintenance Overview Section 9 NOTES ...
Страница 532: ...11 2 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Quality Control Overview Section 11 NOTES ...
Страница 558: ...11 28 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Quality Control References Section 11 NOTES ...
Страница 560: ...12 2 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Printers Overview Section 12 NOTES ...
Страница 566: ...13 2 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Sample Loader Overview Section 13 NOTES ...
Страница 590: ...14 2 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Reticulocyte Package Overview Section 14 NOTES ...
Страница 662: ...A 4 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Bar Codes Overview Appendix A NOTES ...
Страница 666: ...A 8 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Bar Codes Specifications Appendix A NOTES ...