18
08/2007
The scanned image appears in a false-color
presentation (Fig. 22).
If the image is too bright, it appears red on the
screen. Red = saturation (maximum).
If the image is not bright enough, it appears blue
on the screen. Blue = zero (minimum).
Adjusting the laser intensity
"
Set the
Pinhole
to
1
Airy Unit (Fig. 23).
"
Set the
Detector Gain
high.
"
When the image is saturated, reduce AOTF
transmission in the
Laser
control section of the
Channels Tool
(Fig. 23) using the slider to
reduce the intensity of the laser light to the
specimen.
Adjusting gain and offset
"
Increase the
Amplifier Offset
until all blue
pixels disappear, and then make it slightly
positive (Fig. 23).
"
Reduce the
Detector Gain
until the red pixels
only just disappear.
Fig. 22
Image Display
Fig. 23
Channels tool
