08/2007
13
Settings for track configuration in Channel Mode
"
Select
Channel Mode
if necessary (Fig. 15).
"
Click on the
LSM
tab (Fig. 14).
The
Light Path
tool displays the selected track
configuration which is used for the scan procedure.
"
You can change the settings of this panel using the
following function elements:
Activation / deactivation of the excitation wavelengths (check box) and setting of
excitation intensities (slider). If necessary open the
Laser Control
tool (see above).
Selection of the main dichroic beam splitter (HFT) or secondary dichroic beam splitter
(NFT) position through selection from the relevant list box.
Selection of an emission filter through selection from the relevant list box.
Activation / deactivation (via check box) of the selected channel (Ch 1-4, monitor diode
ChM, META detectors ChS1-8, transmission ChD) for the scanning procedure and
assigning a color to the channel.
"
Select the appropriate filters and activate the
channels.
"
Click the
Laser
icon to select the laser lines and
set the attenuation values (transmission in %) in
the displayed window.
"
For the configuration of the beam path, please
refer to the application-specific configurations
depending on the used dyes and markers and
the existing instrument configuration.
"
In the Imaging Setup tool the
Detection Bands
& Laser Lines
are displayed in a spectral panel
(Fig. 16) to visualize the activated laser lines for
excitation (vertical lines) and activated detection
channels (colored horizontal bars).
Fig. 15
Imaging Setup tool for a single track
(LSM)
Fig. 16
Detection Bands & Laser Lines display
