Zeiss KF 2 ICS, Operating Manual, Page: 27 / 40

KF 2
ICS
Carl Zeiss
B 40-028 e 10/97 3-9
Turn the screws (3-4/ 2 ) to superimpose the
dark Ph ring on the bright ring. The dark phase
ring of the objective must fully cover the bright
illumination ring.
A centering telescope (3-5/
1 ) can also be
inserted into the tube instead of a diopter. If a
centering telescope is used, its eyelens must be
shifted until the illuminating ring is in focus.
Insert the eyepiece (3-5/ 3 ) in the tube again.
Adjust the lamp brightness via control (3-3/ 3 ).
If required, place a green filter on the filter
mount (3-3/
4 ) to enhance the contrast of the
phase contrast image.
Optimum phase contrast requires extreme cleanliness! Therefore, clean the front lens of the
objective used, the visible condenser surface, the upper cover slip surface and the lower carrier
plate surface with particular care and make them free from grease.
Fig. 3-6 Illustration of the phase stop
setting