Zeiss Axiovert 200, Quick Start Manual

Page: 5 / 8

18 Feb 2011,<qsg-wf1> p. 5/8
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in "Acquisition Setup" dialogue
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set "Change focus using" to "Zeiss Focus Drive"
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choose and set Z-spacing or number of slices
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set "Auto-focus using" to "Zeiss Focus Drive"
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choose the lightpath where the Autofocus should take its cues from
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set time series
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in "Acquisition Setup" dialogue: set duration
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in video toolbar: set intervals
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set multiple positions
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stage needs to be calibrated for this
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remove sample!!!
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switch to shortest objective (10x, 2,5x or empty position)!!!
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move objective turret to the lowest position!!!
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choose "Calibrate Stage" from the "Stage" menu
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in the "Acquisition Setup" dialogue set "Change XY using" to "Ludl XY
Stage"
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"handmade" positioning
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"Video" menu > "Live Video"
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"Stage" menu > "Clear All Points"
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move to desired point
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"Stage" menu > "Add Point"
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with "Stage" menu > "Review Points" you can adjust the focus for each of
your points separately
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automated positioning
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"Video" > "XY Stage
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"Stage" > "Make Well Overlay"
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"Stage" > "Create Points from Well Overlay"
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follow the instructions
Shutdown procedure
• check if anyone is booked after you within 2 hours
If nobody is booked within two hours:
• remove specimen
• clean oil objective lenses with lens tissue
• Important: close incubation chamber completely (
Æ
protects from dust)
• update time in Sharepoint
• secure your data (e.g. copy them to the server)
• shut down hardware in reverse order from startup