Axiolab 5
HOW TO GET TO THE FIRST PICTURE
ZEISS
07/2018
430037-7014-111
21
Englis
h
11
HOW TO GET TO THE FIRST PICTURE
Setting transmitted-light brightfield according to KÖHLER
−
The microscope has been started up correctly.
−
The microscope has been switched on.
•
Adjust the image brightness using the light
intensity control (
1
) on the microscope
stand.
•
Put a high-contrast sample into the specimen
holder of the mechanical stage.
•
If condensers with a swiveling front lens are
used, swivel these into the beam path with
≥
10x objectives and turn the knurled knob for
vertical adjustment of the condenser (
3
)
to the top stop. Make sure the stop is adjusted
to prevent the condenser from lifting out the
sample.
•
On condensers with revolver/modulator disks:
turn the knurled ring (
3
) to position
H
(or
B
= brightfield.
•
Bring the 10x objective into position on the
nosepiece (
6
) and focus the sample using
the focus drive (
2
).
•
Close the field diaphragm (
5
) enough to
make it visible in field of view (even if blurred)
(Fig. 6/
A
).
•
Lower the condenser using the knurled knob
for vertical adjustment until the edge of the
field diaphragm appears sharp (
B
).
•
Center the field diaphragm image with both
centering screws (
4
) on the condenser
carrier (
C
).
•
Open the field diaphragm enough to make the edge of the diaphragm disappear from the field of
view (
D
).
•
To adjust the aperture (contrast), take an eyepiece from the tube barrel. Looking through the barrel
adjust the aperture with the adjusting lever (
4
) of the aperture diaphragm to the size of approx.
2/3 - 4/5 of the diameter of the objective exit pupil (
E
). In most cases this aperture gives the best
contrast at almost full resolution and is thus the best compromise for the human eye.
•
Replace the eyepiece in the tube barrel.
Fig. 6
Microscope adjustment in
transmitted light brightfield
microscopy