START-UP
Photomicrography with
Carl Zeiss
Attachment of 35 mm SLR cameras
35 mm SLR cameras
2-10
B 40-046 e 03/99
2.4
Determination of the Exposure Time Range for Unblurred Photographs
The limits of the exposure time range for unblurred photographs depend on the used SLR camera, the
microscope adaptation, the microscope magnification and the microscope stand to which the camera is
attached.
These limits for unblurred photographs can be determined as follows:
•
Place a suitable object on the stage (high-contrast specimen featuring large and small object details,
testing grid or stage micrometer).
•
Swing in 10
×
objective.
•
Focus on the object.
•
Set brightfield illuminator according to KÖHLER.
•
Make an exposure series:
trigger one photograph each with the exposure times 1/250s - 1/125s - 1/60s - 1/30s - 1/15s - 1/8s -
1/4s - 1/2s and 1s in the "Automatic" mode.
The different exposure times must be set by adjusting the lamp voltage and, if required, by insertion
of neutral density filters into the illumination beam path.
•
Make a second exposure series using the 40
×
objective.
•
Evaluate the photographs after the film has been developed:
The photographs featuring high contrast and clearly defined object details are blurless.
The exposure time of the first photograph featuring high contrast and clearly defined object details is
the border exposure time of the exposure time range for unblurred photographs.
All the photographs with exposure times
≥
the border exposure time are unblurred.
