INTRODUCTION
Photomicrography using
Carl Zeiss
Contents
35 mm SLR cameras
0-4
B 40-046 e 03/99
CHAPTER 2
START-UP.................................................................................................................. 2-1
2 Start-Up ..................................................................................................................... 2-2
2.1
Unpacking the Photomicrography Components........................................................... 2-2
2.2
Insertion of Photo Reticles MC 2.5
×
............................................................................. 2-2
2.2.1
Assignment of Photo Reticles MC 2.5
×
........................................................................ 2-2
2.2.2
Insertion of MC 2.5
×
Photo Reticles in the Eyepiece ..................................................... 2-4
2.2.3
Focusing of Eyepieces through the Binocular tube ....................................................... 2-4
2.3
Attachment of 35 mm SLR Cameras............................................................................ 2-5
2.3.1
Attachment of 35 mm SLR Cameras with SLR Adapter 2.5
×
to the
60 mm Camera Port ................................................................................................... 2-5
2.3.2
Attachment of 35 mm SLR Cameras with Objective f' = 63 mm to 60 mm
and 40 mm Camera Ports ........................................................................................... 2-6
2.3.3
Attachment of 35 mm SLR Cameras with 2.5
×
/ T2 Adapter to Front Port of
Axiovert 25 C / CFL / CA ............................................................................................. 2-7
2.3.4
Attachment of 35 mm SLR Cameras to Front Camera Port of Axiovert 35 / 35 M /
135 / 135 M ............................................................................................................... 2-8
2.3.5
Attachment of 35 mm SLR Cameras with Objective f' = 63 mm to Camera Ports with
40 mm Clamping Diameter ......................................................................................... 2-9
2.4
Determination of the Exposure Time Range for unblurred Photographs ...................... 2-10
CHAPTER 3
OPERATION .............................................................................................................. 3-1
3 OPERATION ................................................................................................................ 3-1
3.1
Adaptation and Functions ........................................................................................... 3-2
3.2
Photomicrography with 35 mm SLR Cameras .............................................................. 3-3
3.2.1
Photography in a few Steps ........................................................................................ 3-3
3.2.2
Setting the Color Temperature to 3200 K and setting the Image Brightness ................. 3-4
3.2.3
Selecting the Film ....................................................................................................... 3-4
3.2.4
Exposure Correction depending on the Microscopy Technique ..................................... 3-5
3.2.5
Setting and Focusing of Image Section ........................................................................ 3-6
3.2.6 Exposure
Functions ..................................................................................................... 3-7
3.2.7
Trigger the Exposure ................................................................................................. 3-10
3.2.8 Further
Notes............................................................................................................ 3-11
3.2.8.1 Avoid
Blurs ............................................................................................................... 3-11
3.2.8.2
Color Balance in Color Reversal Films......................................................................... 3-11
3.2.8.3
Darkfield and Fluorescence Photography ................................................................... 3-13
3.2.8.4
Computing the Magnification ................................................................................... 3-14
3.2.8.5 Further
Filters............................................................................................................ 3-14
3.3
Using Digital Cameras............................................................................................... 3-15