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Photomicrography with
OPERATION
35 mm SLR cameras
Contents
Carl Zeiss
B 40-046 e 03/99
3-1
OPERATION
Contents
3
OPERATION ...................................................................................................................3-2
3.1
Adaptation and Functions ................................................................................................3-2
3.2
Photomicrography with 35 mm SLR Cameras ...................................................................3-3
3.2.1
Photography in a Few Steps .............................................................................................3-3
3.2.2
Setting the Color Temperature to 3200 K and Setting the Image Brightness......................3-4
3.2.3
Selecting the Film ............................................................................................................3-4
3.2.4
Exposure Correction Depending on the Microscopy Technique..........................................3-5
3.2.5
Setting and Focusing of Image Section .............................................................................3-6
3.2.6
Exposure Functions ..........................................................................................................3-7
3.2.7
Trigger the Exposure......................................................................................................3-10
3.2.8
Further Notes ................................................................................................................ 3-11
3.2.8.1
Avoid Blurs .................................................................................................................... 3-11
3.2.8.2
Color Balance in Color Reversal Films.............................................................................. 3-11
3.2.8.3
Darkfield and Fluorescence Photography ........................................................................ 3-13
3.2.8.4
Computing the Magnification ........................................................................................ 3-14
3.2.8.5
Further Filters ................................................................................................................ 3-14
3.3
Using Digital Cameras.................................................................................................... 3-15
List of illustrations
Fig. 3-1
Use of bulk film ...............................................................................................................3-5
Fig. 3-2
Measuring range of 35 mm SLR camera, ISO 100 / 21°, camera lens f / 1.4
with apertures k = 1.4 - 16 ..............................................................................................3-8
Fig. 3-3
Measuring range of 35 mm SLR camera with T2-Adapter, ISO 100 / 21° ...........................3-9