WPI UltraPath 2 System Manual Download

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SYSTEM GUIDE

for visible and ultraviolet (UPUV-2) systems

UltraPath 2

Multiple pathlength sample cell for absorbance

spectroscopy with extended dynamic range

Serial No._____________________

www.wpi-europe.com

180522

Summary of Contents for UltraPath 2

Page 1: ...GUIDE for visible and ultraviolet UPUV 2 systems UltraPath 2 Multiple pathlength sample cell for absorbance spectroscopy with extended dynamic range Serial No _____________________ www wpi europe com...

Page 2: ...ity of the UltraPath 2 sample cell 18 salinity matched reference solutions to avoid baseline offsets 18 Measurement reliability 18 Flow rate and maximum pressure 18 Effective pathlength and linearity...

Page 3: ...ove any safety devices installed this will void your warranty and create an unsafe operating condition Warning Dangerous voltages are present No user serviceable parts inside unit Instrument should be...

Page 4: ...laboratory and in the field i e at sea CDOM concentrations vary significantly between open ocean samples with low CDOM e g 0 007 m 1 at 380 nm and high CDOM freshwater environments e g 10 20 m 1 at 3...

Page 5: ...e Sample Injector and through the UltraPath 2 sample cell The spectrophotometer is connected to the network via an ethernet port The TIDASDAQ SpectraView software included is installed on a standard W...

Page 6: ...f the UltraPath 2 can be switched between 10 50 and 200 cm Pull the knob and turn to the selected pathlength 5 UltraPath 2 Sample Injector Kit The Sample Injector WPI 58006 is connected to the Liquid...

Page 7: ...upplied For further instructions refer to TIDAS S 300 UV VIS instruction manual 13 LAN RJ45 ethernet communication interface The TIDAS S 300 UV VIS is connected to a network via the supplied ethernet...

Page 8: ...2 fiber optic switch 4 into the TIDAS S 300 UV VIS spectrometer Input 11 with a 600 m core fused silica fiber WVLUXDUV S 0600 SMA Peri Star Pro Peristalitic Pump 20 Peri Star Pro Pump Head Two channe...

Page 9: ...nstruction manual for details Cleaning Kit Available US Only 26 Waveguide Cleaning Kit The waveguide cleaning kit contains three solutions specifically developed to keep the UltraPath 2 sample cell cl...

Page 10: ...nnector D hole 58006 1 Sample Injector Assembly for LWCC 58450 1 Kit Adapter Syringe LWCC 89372 2 1 LWCC Injection System LOOP 5ML 1 Sample Loop Kit 5 mL 2 5m PFA Tubing 1 16 ID x 1 8 OD 2 x PEEK fitt...

Page 11: ...11 P a g e inspection requested Please read the section entitled Claims and Returns on the warranty page of this manual Please call WPI Technical Support if any parts are missing...

Page 12: ...45 connector 6 Connect the Power cord supplied with the to the TIDAS S 300 UV VIS to the Input Terminal 16 of the TIDAS S 300 UV VIS and the mains 7 Assemble the sample injector loop of the UltraPath...

Page 13: ...spectroscopic data recording See the LWCC Injection System Manual for connection details The QFT2 and CUV2012 1 are not part of the standard setup They are used when you need to measure particulate a...

Page 14: ...000 m 100 cm SMA WVLUXUVIS S 0600 100 SMA 1 UVIS Fiber 200 1200nm 600 m 100 cm SMA QFT2 1 25 mm Glass Fiber Filter GF F and 10mm Cuvette Holder CUV2012 1 1 Quartz Cuvette Style C 12 4x12 4x45 mm 10 mm...

Page 15: ...ide by vacuum suction Select a pathlength 10 50 200 cm at the fiber optic switch 4 of the UltraPath 2 sample cell How to fill the UltraPath 2 sample cell Place the injector 5 into a sample vial The sa...

Page 16: ...are started Measurements can then be taken by the following steps 1 Define Directory Filename and Comment of your dataset 2 Press the Settings icon to open the TIDASDAQ Parameter window Use the follow...

Page 17: ...rved until the sample cell is filled with the sample solution then a steady signal will be present This may take between 15 seconds and 1 minute depending on the pathlength used It is good practice to...

Page 18: ...be within 1 2 mAU between baseline samples If larger baseline variations are observed the following problems may be present 1 An air bubble is trapped inside the cell Allow for more sample to be inje...

Page 19: ...rs a linear relationship to the concentration of an analyte WPI s sample cells based on Liquid Waveguide Technology were extensively tested and proved to be linear over a range 0 01 to 2 0 AU limited...

Page 20: ...ned by filtering a known amount of sample through a Glass Fiber Filter GF F and measuring the particulate absorption coefficient ap concentrated on the filter This technique is called quantitative fil...

Page 21: ...n the filter holder of the QFT2 and take an absorbance reading as described in the TIDAS DAQ3 user manual Save the scan to your hard drive import the data into e g Excel and calculate the corrected pa...

Page 22: ...hed in the following papers Mitchell B G Algorithms for Determining the Absorption Coef cient of Aquatic Particles Using the Quantitative Filter Technique QFT SPIE Vol 1302 Ocean Optics X 1990 137 148...

Page 23: ...described below The following cleaning cycle has been optimized for organic e g CDOM type contamination however depending on your contaminant the cleaning cycle may have to be altered For thorough cl...

Page 24: ...ient to clean the cell or organic contamination In extreme cases for example CDOM left in the UltraPath 2 cell overnight it was found that filling the waveguide with Solution 1 and letting it sit for...

Page 25: ...tion times given in the calibration sheets See Operation Instructions for details Confirm the results with the calibration sheets If the light intensities are significantly lower than on the calibrati...

Page 26: ...e tubing is not pressed at the rollers in the pump head Silicone tubing is not properly set in the PeriStar Pro pump head See PeriStar Pro instruction manual how to set ins tall silicone tubing in the...

Page 27: ...cell is contaminated Fill the UltraPath 2 with de ionized water and disconnect the fiber at the fiber optic switch of the UltraPath 2 Check if light exits at the 10 50 or 200 cm output This light wil...

Page 28: ...and redo experiment with different integration times until successful Using the QFT 2 there is still no light at the TIDAS S 300 UV VIS detector Fibers not properly connected to TIDAS S300 and the QF...

Page 29: ...d Feed Thru Connector D hole 58006 Sample Injector Assembly for UltaraPath2 58450 Kit Adapter Syringe LWCC 500320 Silicone tubing 1m length 1 6 mm I D 1 6 mm wall thickness CUV2012 1 Quartz Cuvette St...

Page 30: ...20 nm Inner diameter 1 mm Cell volume 2 4 mL Sample in let outlet Standard 10 32 Coned Port Fitting Fiber input 600 m core diameter SMA Fiber Output 600 m core diameter SMA Solvent resistance Most org...

Page 31: ...illary flow cell Deep Sea Research I Vol 47 2000 1157 1171 Mathias Belz Peter Dress Aleksandr Sukhitskiy and Suyi Liu Linearity and effective optical pathlength of liquid waveguide capillary cells Par...

Page 32: ...eguides and Long Path Absorbance Detection Anal Chem 1998 Vol 70 pp 4661 4669 Li Song S Liu V Zhelyaskov and M A El Sayed Application of liquid waveguide to Raman spectroscopy in aqueous solution Appl...

Page 33: ...data or property that may be caused directly or indirectly by use of this product Claims and Returns Inspect all shipments upon receipt Missing cartons or obvious damage to cartons should be noted on...

Page 34: ...wpiuk wpi europe com Germany Pfingstweide 16 D 61169 Friedberg Hessen Germany Tel 49 0 6031 67708 0 Fax 49 0 6031 67708 80 E mail wpide wpi europe com China Hong Kong WPI Shanghai Trading Co Ltd Rm 2...

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