UltraMicroPump II
World Precision instruments
15
note that this does not yet affect the action of any channel since
M
has not been
selected as the device type for any channel. Before proceeding, move the cur-
sor back to the Volume set field and re-enter the correct volume for the syringe on
output channel 1 (which may be any type — not necessarily type m).
Special Syringe Considerations ILS005
the umPii-micro4 was designed around 60 mm or 54.1mm syringes. the full
injection of a 5 µl syringe is expected to be a 60 mm movement. in the case of this
ils special 5 µl glass luer tip syringe this length is 27 mm for an injection of 5 µl.
there are two solutions:
Solution 1.
using type “c” (the 54.1 mm 5 µl syringe), multiply the volume
to be injected by 27/54.1 or (0.4990).
example. A 2500 nl injection using type c should be entered into the micro4
as 2500 × 0.4990 or 1247.5 nl. this value can be further calibrated see page
17 for details.
Solution 2.
using type m, divide the type c nl/step number by 0.4990.
example: the standard 54.1 mm syringe has a value of 0.2934 nl/step on
a 5 µl syringe. the ils005 has a 27mm stroke for this same full volume.
0.2934 / 0.4990 = 0.5880 is the nl / step value to enter following the type
m instructions on this page. (0.5880 is also verified by the formulas on the
previous page.)
After defining your syringe in micro4’s memory, move the cursor to the device type
field of the output channel to which that pump is connected and press
select
until
M
appears.
Any number of channels may use type
M
syringes, but
since a single definition for type
M
is stored in micro4 all
M
devices must be identical. (that is, you cannot use
two non-standard types, such as 2.5 µl and 0.25 µl.)
the minimum delivered volume depends on the syringe
size and is listed in the syringe type table under Volume
per step. the actual volume delivered is divisible by the volume per step. For
example, using a syringe with a Volume per step of 1 nl, actual delivered volume
for the given set volume is listed below.
Summary of Contents for UltraMicroPump II
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