WPI UltraMicroPump II, Instruction Manual

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UltraMicroPump II
World Precision instruments
14
Defining Other Syringes
eleven microsyringes with volumes ranging from 0.5 µl to 1000 µl are already
preset in the micro4 (syringe types
A through L — see table on page 11). A mi-
crosyringe with a volume other than those preset may be entered as device type
M . However, the Volume per step of the type M syringe must be defined for the
micro4. NOTE: there is only one memory location to define a type m value for all
four pumps.
First, calculate the Volume per step using the formula below. syringe displace-
ment is the distance between 0 and the maximum volume marked on the syringe in
inches. syringe volume is in nanoliters. id is in millimeters.
Syringe of known ID
Volume per step = (
ID
/2) x (
ID
/2) x 3.1415926 x 1000 x 0.003175
Syringe of unknown ID
the inside diameter (id) of a syringe can be determined by this formula:
id = sQrt(
Volume
/(3.14159 x
Length
)) x 2
where length is the visible marking on the syringe body (total length in mm) and
Volume is the full volume of the syringe in microliters.
For example, if you have a 60 mm syringe with a
2.5 µl volume, use the formula above to determine
its Volume per step. move the cursor to the Volume
set field of output channel 1 and enter the calculated
value (in this case, 0.1323 nl):
then use the
left arrow
key to scroll the cursor to the
first position on the lcd display and press . this sets your calculated definition
for your type
M syringe into the micro4’s memory.
Calculate in Microsoft
Excel — just enter
these formulae into
a spreadsheet,
replacing Volume,
Length, and ID
from your own
measurements.