WPI UltraMicroPump II, Instruction Manual

Page: 13 / 32

UltraMicroPump II
World Precision instruments
11
Type
Syringe Volume
(defined for this length)
ID (mm) nL / step REV J
Max. Rate
nl /sec or
nl /min
A 0.5 µl (54.1 mm) † 0.1085 (0.102 reV H) 0.0294 ( 0.026 rev H) 20
B 1.0 µl (54.1 mm) † 0.1534 (0.145 reVH) 0.0587 (0.052rev H) 40
C** 5 µl (54.1 mm) † 0.343 (0.325 reV H) 0.2934 (0.263 rev H) 202
D 10 µl (54.1 mm) † 0.485 (0.46 reV H) 0.5868 (0.528 rev H) 406
E 25 µl (60.0 mm) 0.73 1.329 1022
F 50 µl (60.0 mm) 1.03 2.646 2035
G 100 µl (60.0 mm) 1.46 5.315 4088
H 250 µl* (60.0 mm) 2.3 13.191 9999
I 500 µl* (60.0 mm) 3.26 26.501 9999
J 1000 µl* (60.0 mm) 4.61 52.995 9999
K
nanoliter 2000
† †
0.48 plunger in 0.50 glass 2.3 nl /step (0.0005” step) 884
L 10 µl (60 mm) 0.4607 (0.100 rev H) 0.5293 (0.0249 rev H) 170
M user defined see page 12
** ils005 (27 mm) 0.4856
0.5880 compensates for length as
tYPe m
* Gas-tight syringes are not recommended for UMPII in these volumes; instead, use a liquid-tight
syringe to prevent drive motor damage.
** The ILS005 5 µL syringe must use type M with a 0.5880 nL / step entry. See page 15 for details.
† These are defined as 60 mm in Rev. H firmware.
† †
WPI’s
Nanoliter 2000
, a nanoliter injector for the 2-70 nL range, comes with its own simple controller
but may also be driven by the Micro4. For more information, enquire about WPI #
B203XVY
.
Note On Syringe Stroke Length
the delivery of the umPii is based on 60 mm or 54.1 mm syringes. Please note
which syringe length you are using as a factor of 0.9016 may need to be applied to
the volume to be injected in or-
der to have a precise injection.
For the ils005, use the tYPe
“m” values in the above table.
Maker Syringe
Stroke
Length
Use TYPE
Hamilton 1700 series ,10 µl 60 mm L
Hamilton 700 series,10 µl 54.1 mm
D
sGe 0.5 µl – 10 µl 54.1 mm A,B,C,D
ils 5 µl luer tip ils005 28 mm M
sGe 25 µl – 500 µl 60 mm
E - L
WPi FlexiFil™ 54.1 mm D
WPi nanoFil™ 60 mm L
*Manufacturers have the right to
change this specification at any time.