WPI STX2-PLUS, Instruction Manual

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STX2-PLUS
World Precision Instruments 1
ABOUT THIS MANUAL
The following symbols are used in this guide:
This symbol indicates a CAUTION. Cautions warn against actions that can cause
damage to equipment. Please read these carefully.
This symbol indicates a WARNING. Warnings alert you to actions that can cause
personal injury or pose a physical threat. Please read these carefully.
NOTES and TIPS contain helpful information.
Fig. 1—The STX2-PLUS electrode is designed for use with the EVOM3 meter.
INTRODUCTION
The STX2-PLUS electrode can be used with the EVOM3 and is designed to facilitate
measurements of membrane voltage and resistance (TEER) of cultured epithelia
directly in 12- and 24-well tissue culture plates. The electrode incorporates a
adjustable pair of probes, 5 mm tall, 4 mm wide and 0.7 mm in thickness. Each probe
has an outer (current) and an inner (voltage) electrode.
For 12- or 24-well measurements, the
STX2-PLUS electrode is capable of making
precise measurements. The STX2-PLUS is designed primarily for 24-well inserts, and
it can be used with 24-well HTS plates (Corning 3378) and 12-well inserts (Corning
3460) with good results, as long as the placement is consistent. Consistent electrode
placement and mechanical stability are essential for consistent resistance results. Try
to keep the fluid levels and fluid temperatures constant. The use of a warming plate
can add to measurement items and reduce CO
2
out gassing during readings. CO
2
out
gassing can change the pH of the media and sharply increase a resistance reading.