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STX2-PLUS

World Precision Instruments 

9

APPENDIX A: KCL TESTING MIXTURES

When making a variety concentrations of KCl solution, use the following table as a 

guide. Start by making a solution of 160 mM concentration. To make 100 mL of 160 

mM KCl:
Add 1.192 gm KCl to 100 mL of distilled water. (1M KCl = 74.54 gm/M in 1 L DI water)

Use

Add DIW

Results

50 mL of 160 mM KCl

50 mL

100 mL 80 mM KCl

50 mL of 80 mM KCl

50 mL

100 mL 40 mM KCl

50 mL of 40 mM KCl

50 mL

100 mL 20 mM KCl

25 mL of 20 mM KCl

25 mL

50 mL 10 mM KCl

APPENDIX B: COMPATIBLE INSERTS AND PLATES

Corning

Millipore

Material

Membrane 

Diameter (mm)

Growth Surface 

Area (cm

2

)

Membrane 

Pore Size (μm)

3470

6.5

0.33

0.4

3472

PITP01250

6.5

0.33

3.0

3413

PCF Insert

6.5

0.33

0.4

3415

PITP01250 

PCF Insert

6.5

0.33

3.0

3421

6.5

0.33

5.0

3422

PIEP01250 

PCF Insert

6.5

0.33

8.0

3495

PIHT12R48* 

PET Insert

6.5

0.33

0.4

PIHA01250

HA Insert

6.5

0.33

0.45

PICM01250

CM Insert

6.5

0.33

0.4

3496

PISP12R48* 

PET Insert

6.5

0.33

3.0

PIRP12R48 

PET Insert

6.5

0.33

1.0

PIMP12R48* 

PET Insert

6.5

0.33

5.0

PIEP12R48* 

PET Insert

6.5

0.33

8.0

PIXP01250 

PCF Insert

6.5

0.33

12

PIHP01250

1.0

PITT01250

3.0

* Tri-supports

Summary of Contents for STX2-PLUS

Page 1: ...WORLD PRECISION INSTRUMENTS STX2 PLUS for use with EVOM3 Epithelial Voltohmmeter Serial No _____________________ www wpiinc com INSTRUCTION MANUAL 082620...

Page 2: ......

Page 3: ...NG INSTRUCTIONS 3 Positioning Electrode in a Culture Cup 3 Stable Repeatable Readings 3 Ease of Use 3 Adjusting the Length of the Electrodes 4 MAINTENANCE 5 Cleaning Maintaining the STX2 PLUS Electrod...

Page 4: ...ii World Precision Instruments...

Page 5: ...rectly in 12 and 24 well tissue culture plates The electrode incorporates a adjustable pair of probes 5 mm tall 4 mm wide and 0 7 mm in thickness Each probe has an outer current and an inner voltage e...

Page 6: ...any parts are missing at 941 371 1003 or customerservice wpiinc com Returns Do not return any goods to WPI without obtaining prior approval RMA required and instructions from WPI s Returns Department...

Page 7: ...ility of the measurements is significantly improved Fig 2 Right Electrodes in solution Stable Repeatable Readings This new electrode design sets the correct depth and centering of the electrode elemen...

Page 8: ...a hanging insert Fig 6 Rotate the nut to adjust the length of the electrodes Fig 7 The electrode length is properly adjusted 2 Align the electrodes with the well inserts and position the STX2 PLUS ove...

Page 9: ...to your maintenance schedule and the frequency of your cleaning Soak the electrodes overnight when the electrodes have not been on a routine maintenance cleaning schedule Soak 60 minutes if you are o...

Page 10: ...us electrode must be dipped into chlorine bleach 5 sodium hypochlorite on occasion to maintain the amount of Ag to AgCl balance Otherwise the electrode will become unstable A 10 15 minute immersion of...

Page 11: ...should show an R2 value 0 99x NOTE These resistance readings can vary due to fluid volume and electrode depth These values are a reference An Excel scatter plot of the molarity vs resistance as a pow...

Page 12: ...ng Radio frequency interference Turn off or move any cellular phones farther away from the experimental setup NOTE If you have a problem issue with that falls outside the definitions of this troublesh...

Page 13: ...25 mL of 20 mM KCl 25 mL 50 mL 10 mM KCl APPENDIX B COMPATIBLE INSERTS AND PLATES Corning Millipore Material Membrane Diameter mm Growth Surface Area cm2 Membrane Pore Size m 3470 6 5 0 33 0 4 3472 PI...

Page 14: ...10 World Precision Instruments Millicell Membrane Pore Size m Qty Pkg MCHT24H48 0 4 48 MCRP24H48 1 0 48 MCSP24H48 3 0 48 MCMP24H48 5 0 48 MCEP24H49 8 0 48...

Page 15: ...special or consequential arising from a failure of this product to operate in the manner desired by the user WPI shall not be liable for any damage to data or property that may be caused directly or i...

Page 16: ...Tel 49 0 6031 67708 0 Fax 49 0 6031 67708 80 E mail wpide wpi europe com China Hong Kong WPI Shanghai Trading Co Ltd Rm 27A Liangfeng Building No 8 Dongfang Road Pudong New District Shanghai PR China...

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