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World Precision Instruments

TROUBLESHOOTING

Issue

Possible Cause

Solution

Electrode 

tips 

over when  released

The cable is heavy and can cause 

the electrode to tip over if too much 

cable is in the air.

Lay the cable on the tabletop as close to the 

electrode head as possible.

The reading appears to  be drifting* (see the drift 

definition below).

The electrodes are coated.

The Electrodes requires enzymatic cleaning.

The fluid temperature in the plate is 

changing.

Use a plate warmer.

 In a 5% CO

2

 environment, a loss of 

CO

2

 causes the media pH to change, 

and the resistance reading may 

increase sharply.

A 5% CO

2

 environment can help in reducing pH 

media changes. A plate warmer can reduce out 

gassing.

The electrode reading is  unstable. (see the stabil

-

ity definition below).

The electrodes are coated.

The electrodes require enzymatic cleaning.

The electrodes need to be chlorided. The electrodes require chloriding

Radio frequency interference

Turn off or move any cellular phones farther 

away from the experimental setup. 

NOTE

: If you have a problem/issue with that falls outside the definitions of this 

troubleshooting section, contact the WPI Technical Support team at 941.371.1003 or 

[email protected].
* Defining Term:

• 

Drift–Readings that continuously increase or decreases a significant value (either 
voltage or resistance) over time. Example 1: At 10 mV the Pd reading is increasing 
1 mV/minute. (A drift of 0.1 mV/minute is acceptable.) Example 2: At 1000 Ω 
the reading is increasing 100 Ω/minute. (A drift of 10 Ω/minute is acceptable.) 
Excessive drift may be caused by a coated electrode, pH or temperature changes. 
See “Cleaning/Maintaining the STX2-PLUS Electrodes” on page 5. 

• 

Instability–At 500 Ω, the reading jumps from 450 to 550 Ω and will not settle down 
(an instability ±5 Ω is acceptable in the 500 Ω range. In the higher ranges, up to 
±1000 Ω is acceptable at the 100K range. Electrodes showing instability may be 
coated with DMEM and require enzymatic cleaning. See “Cleaning/Maintaining the 
STX2-PLUS Electrodes” on page 5.  

Summary of Contents for STX2-PLUS

Page 1: ...WORLD PRECISION INSTRUMENTS STX2 PLUS for use with EVOM3 Epithelial Voltohmmeter Serial No _____________________ www wpiinc com INSTRUCTION MANUAL 082620...

Page 2: ......

Page 3: ...NG INSTRUCTIONS 3 Positioning Electrode in a Culture Cup 3 Stable Repeatable Readings 3 Ease of Use 3 Adjusting the Length of the Electrodes 4 MAINTENANCE 5 Cleaning Maintaining the STX2 PLUS Electrod...

Page 4: ...ii World Precision Instruments...

Page 5: ...rectly in 12 and 24 well tissue culture plates The electrode incorporates a adjustable pair of probes 5 mm tall 4 mm wide and 0 7 mm in thickness Each probe has an outer current and an inner voltage e...

Page 6: ...any parts are missing at 941 371 1003 or customerservice wpiinc com Returns Do not return any goods to WPI without obtaining prior approval RMA required and instructions from WPI s Returns Department...

Page 7: ...ility of the measurements is significantly improved Fig 2 Right Electrodes in solution Stable Repeatable Readings This new electrode design sets the correct depth and centering of the electrode elemen...

Page 8: ...a hanging insert Fig 6 Rotate the nut to adjust the length of the electrodes Fig 7 The electrode length is properly adjusted 2 Align the electrodes with the well inserts and position the STX2 PLUS ove...

Page 9: ...to your maintenance schedule and the frequency of your cleaning Soak the electrodes overnight when the electrodes have not been on a routine maintenance cleaning schedule Soak 60 minutes if you are o...

Page 10: ...us electrode must be dipped into chlorine bleach 5 sodium hypochlorite on occasion to maintain the amount of Ag to AgCl balance Otherwise the electrode will become unstable A 10 15 minute immersion of...

Page 11: ...should show an R2 value 0 99x NOTE These resistance readings can vary due to fluid volume and electrode depth These values are a reference An Excel scatter plot of the molarity vs resistance as a pow...

Page 12: ...ng Radio frequency interference Turn off or move any cellular phones farther away from the experimental setup NOTE If you have a problem issue with that falls outside the definitions of this troublesh...

Page 13: ...25 mL of 20 mM KCl 25 mL 50 mL 10 mM KCl APPENDIX B COMPATIBLE INSERTS AND PLATES Corning Millipore Material Membrane Diameter mm Growth Surface Area cm2 Membrane Pore Size m 3470 6 5 0 33 0 4 3472 PI...

Page 14: ...10 World Precision Instruments Millicell Membrane Pore Size m Qty Pkg MCHT24H48 0 4 48 MCRP24H48 1 0 48 MCSP24H48 3 0 48 MCMP24H48 5 0 48 MCEP24H49 8 0 48...

Page 15: ...special or consequential arising from a failure of this product to operate in the manner desired by the user WPI shall not be liable for any damage to data or property that may be caused directly or i...

Page 16: ...Tel 49 0 6031 67708 0 Fax 49 0 6031 67708 80 E mail wpide wpi europe com China Hong Kong WPI Shanghai Trading Co Ltd Rm 27A Liangfeng Building No 8 Dongfang Road Pudong New District Shanghai PR China...

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