SI-BF-100
World Precision Instruments
5
measurement) only require one photomultiplier. Others (like the ATPase
measurement) require both photomultipliers.
BNC Outputs
: The analog outputs can be connected to a data acquisition system.
They provide a normalized (relative) output based on the LED intensity and the PMT
( photomultiplier) gain factor. The output depends on the system application. For
example, for calcium measurement:
•
Output A–Normalized output of the two 340nm LED modules
•
Output B–Normalized output of the 380nm LED module
•
Output A/B–Ratio of Output A to Output B
NOTE
: The display shows the actual ratio of the emissions measured from the two
frequencies of light. To calculate the ratio based on the A/B BNC voltage output, use
the formula:
Ratio =
A/B Voltage x 2
Gain Adjustment Factor
Display
–Press the
Display
button to toggle between the system parameters and the
live ratio measurements. Press the
Setup
button to enter the Confi guration mode
and change the system setup.
Fig. 4—The display (left side) shows the actual ratio measurement. The six control buttons
(right side) let you setup the confi guration parameters, turn the LEDs off and on, and
manipulate the display.
Control Buttons
–There are six control buttons.
•
Display
–Press this button to toggle the display between parameters and
ratio measurement.
•
Setup
–Press this button to enter the setup mode and change parameters.
Adjustable parameters include sampling frequency, gain adjustment,
photomultiplier gain voltage and fi lter frequency.
•
Module Confi g
–Press this button to confi gure any of the LED modules.
From this menu, you can enable/disable a module, set the current output,
set the delay before it illuminates and set the length of the period that the
LED will illuminate.
•
LED On/Off
–Press this button to turn all the enabled LED modules on or
off. When the enabled modules are on, the LED light fl ashes. When you are
