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SI-BF-100

World Precision Instruments 

3

Warnings

CAUTION

: The optical input feeds light into the  photomultiplier, which is an 

extremely sensitive light measurement device. To avoid damage to the 
photomultiplier, it is imperative that the probe be attached to the optical 

input or that the input be capped when the 

SI-BF-100

 is powered on. 

PHOTOMULTIPLIERS ARE EXPENSIVE TO REPLACE.

 Parts List

After unpacking, verify that there is no visible damage to the system. Verify that all 
items are included:
(1)

 

SI-BF-100 Biofl uorometer

(1) 12V Power adapter
(1) Species-specifi c probe (for example, calcium or ATPase)
(1) 

M3301

 Manual Manipulator

(1) 

M10

 Magnetic Stand

(1) Instruction Manual

 Unpacking

 Upon receipt of this instrument, make a thorough inspection of the contents and 
check for possible damage. Missing cartons or obvious damage to cartons should be 
noted on the delivery receipt before signing. Concealed damage should be reported 
at once to the carrier and an inspection requested. Please read the section entitled 
“Claims and Returns” on page 27 of this manual. Please contact WPI Customer Service 
if any parts are missing at 941.371.1003 or [email protected].

 

Returns:

 Do not return any goods to WPI without obtaining prior approval (RMA 

# required) and instructions from WPI’s Returns Department. Goods returned 
(unauthorized) by collect freight may be refused. If a return shipment is necessary, 
use the original container, if possible. If the original container is not available, use a 
suitable substitute that is rigid and of adequate size. Wrap the instrument in paper 
or plastic surrounded with at least 100mm (four inches) of shock absorbing material. 
For further details, please read the section entitled “Claims and Returns” on page 
27 of this manual.

Summary of Contents for SI-BF-100

Page 1: ...SI BF 100 Bio uorometer for Fluorescence Imaging World Precision Instruments INSTRUCTION MANUAL Serial No _____________________ 061413 www wpiinc com...

Page 2: ...Mode 11 Con guring the System 12 Modifying the Sampling Frequency 12 Modifying the Gain Adjust 12 Modifying the Photomultiplier Gain Voltage 13 Modifying the Filter Frequency 13 Setting up LED Modules...

Page 3: ...iv World Precision Instruments...

Page 4: ...ibed in this manual are examples of what can be accomplished with this incredibly exible Bio uorometer For custom con gurations please contact WPI at sales wpiinc com Until now bio uorometers were com...

Page 5: ...of ATPase Activity Measurement on page 23 The table belows outlines the example applications used in this manual Excitation Wavelengths Emission Filters for Bio uorescent Dyes and Molecules Dye or Mo...

Page 6: ...ible damage Missing cartons or obvious damage to cartons should be noted on the delivery receipt before signing Concealed damage should be reported at once to the carrier and an inspection requested P...

Page 7: ...ort ort 1 ort 2 t N N NC Out ut NC Ou NC Ou p LED Module Status Indicator For Future Us Power Button Fig 3 The Bio uorometer control unit is rack mountable Probe Excitation Port Connect the excitation...

Page 8: ...between the system parameters and the live ratio measurements Press the Setup button to enter the Con guration mode and change the system setup Fig 4 The display left side shows the actual ratio measu...

Page 9: ...nnector Connect the 12V power adapter to this port LED Module Monitor Port This port gives you access to each LED module to trigger activity based on a wavelength that is ring It uses a 5 0V TTL pulse...

Page 10: ...an extremely sensitive light measurement device To avoid damage to the photomultiplier it is imperative that the probe be attached to the optical input or that the input be capped when the SI BF 100...

Page 11: ...the chassis Fig 11 4 Use a small hex key to loosen the set screw holding the lter in place Fig 12 Fig 12 Left Use a hex key to loosen the set screw holding the lter in place Fig 13 Right The lter was...

Page 12: ...tracellular calcium Fura 2 measurement system uses three LED modules and one photomultiplier The Bio uorometer can be con gured with up to seven LED modules and two photomultipliers You can add additi...

Page 13: ...le that comes out of the back end of the LED module Loosen the two screws on the sides of the LED module Fig 17 Lift the LED module off the circuit board being careful not to bend the ber optic cable...

Page 14: ...Press the Up or Down button to change the AVG Samples value The default number of samples is 20 Changing the Operating Mode The Bio uorometer has two operating modes single emission and dual emission...

Page 15: ...th of the sampling period In essence it sets how quickly measurements are taken The default is 1000Hz and settings can range from 1 1000Hz 1 Press the Setup button The current sampling frequency is sh...

Page 16: ...n Voltage parameter displays The current PMT1 gain voltage is shown on the display Fig 29 The gain voltage can be set from 0 5 1 0V 2 Press the Up or Down button to change the gain voltage 3 Press the...

Page 17: ...5 Not used Not used Module 6 Not used Not used Fig 31 Press the Module Con g button to reveal the module con guration menu 2 Press the Setup button until the arrow displays next to the parameter you w...

Page 18: ...play Press the Display button to toggle between the two main displays Parameters display Ratio AVG Samples display Fig 33 Left Parameters display Fig 34 Right Ratio Average Samples display Parameters...

Page 19: ...lly when an experiment is under way you will observe the Ratio Sample Averages display screen Ratio The ratio displayed is the actual ratio between the emission frequencies measured Avg Samples Avg Sa...

Page 20: ...AINTENANCE Monitoring the LED Output Sometimes it is helpful to know the output of the individual LED modules For example if you want to balance the power of the excitation frequencies you might want...

Page 21: ...0 Magnetic Base 94689 ATP Probe 94642 Calcium Probe 801513 Power Supply SW Ext 12V 45W for SI BF 100 802232 12 5mm Optical Filter 632nm BP 802233 12 5mm Optical Filter 694nm BP 802234 12 5mm Optical F...

Page 22: ...ers exists the myosin binding sites on the actin laments are covered and the ber contraction stops The intracellular concentration of calcium can be determined by measuring the uorescence of an indica...

Page 23: ...in blue The intrinsic absorbencies of the uorophores are represented by dashed lines The emission spectra are represented by solid lines The optimal site for uorophore excitation occurs at the peak ab...

Page 24: ...citation Indo 1 Ca emission Indo 1 Ca excitation Indo 1 Ca emission 405nm Filter transmission 486nm Filter transmission Fig 39 Excitation emission and ltering of Indo 1 with the SI BF 100 Bio uoromete...

Page 25: ...e concentration ratio of the bound and free calcium in the bers Percent Signal 0 20 40 60 80 100 Wavelength nm 300 350 400 450 500 550 600 650 LED emission Fura 2 Calcuim Bound excitation Fura 2 Calci...

Page 26: ...bridge that is made between the end of a myosin molecule and a binding site on an actin molecule requires a molecule of ATP an energy source to be hydrolyzed when the end of the myosin molecule is rel...

Page 27: ...tween ATPase activity and the conversion of NADH to NAD allows ATPase activity to be measured by the change in the uorescence of the incubation solutions containing NADH NADH is auto uorescent and has...

Page 28: ...issue movement are effectively cancelled The ratio of the two PMT outputs can be recorded directly from the third output on the front of the Bio uorometer When con gured for measurement of ATPase acti...

Page 29: ...Red 20 G gain 1 gain factor 5 12 gain voltage 13 I Indo 1 19 21 22 L LED 5 10 LED module 9 14 17 light sources 1 low pass lter 13 M Module Con g 14 modules 5 Module State 15 muscle tester 4 myosin 19...

Page 30: ...t special or consequential arising from a failure of this product to operate in the manner desired by the user WPI shall not be liable for any damage to data or property that may be caused directly or...

Page 31: ...dshire SG4 0TJ Tel 44 0 1462 424700 Fax 44 0 1462 424701 E mail wpiuk wpi europe com Germany Zossener Str 55 10961 Berlin Tel 030 6188845 Fax 030 6188670 E mail wpide wpi europe com China Hong Kong WP...

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