PV850
World Precision Instruments
19
V=
p
r
2
h=
p
ID
2
/
r
2
•
Thin wall 1 mm glass has a nominal 0.750 µm ID, and a 1 mm length contains
o u (
n )
•
Standard wall
1B100
glass has a 0.580 µm ID, and a 1 mm length holds
o u (
n )
ot t os s a a
to ran an t at
ns on s ta to
making a correct injection of 1 nL.
11. Divide the average found in step 9 by the calculated volume in nL of a 1 mm
length of your pipette found in step 10. This establishes the time in milliseconds
that it takes to inject a known volume. From there you can calculate how many
nanoliters can be injected per second and how many milliseconds it takes to
n t a s n nano t r o u
Multibarrel Microinjection
For injection with a multi-barrel
micropipette, the PolyFil multi-barrel
micropipette coupling kit can be
purchased from World Precision
Instruments. This multi-barrel
micropipette coupling kit allows easy
and secure coupling of a multi-barrel
micropipette to a pressure source. The
ts n u a
ort an o
allows use of a single
PV850
to drive up
to six micropipette barrels
independently.
Fig. 28—PolyFil multibarrel micropipette
coupling kit
MicroFil™
inserted into
each barrel
A drop of hot glue
provides a secure,
leak-free seal.
Pipette pulled from
multi-barrel glass capillary
Multi-barrel micropipette coupling kit
