PV850
World Precision Instruments
15
3. Press the BACK button to save the settings and return to the main screen. A
warning indicates that the Injection Port is disabled.
Fig. 25—When the Injection Port is disabled a notice appears on the main screen.
4. To install the micropipette, see “Assembling the Capillary Kit” on page 9.
5. When the micropipette is properly installed, click on the Settings area to access
the Settings menu and press the Port OFF button to enable the Injection Port.
TECHNIQUES IN MICROINJECTION
The
PV850
as s n or
an n tas s
t
ro n t on o u nto
s
In this section, we will look at several important things to keep in mind when working
with the
PV850
.
Setting the Compensation Pressure
The
o
nsat on r ssur s us to ount r a an t a ar a t on o t u
a
n nto t
tt s ou ns rt an
t
tt nto u ou an s a
meniscus rising from the capillary tip.
Set the Compensation Pressure parameter by touching the Compensation Pressure
parameter on the main screen and adjusting the knob until the meniscus stops at the
s r os t on n t
ro
tt n an a
at ons o or
or uor s nt
s sso
n t n t on u
a ar ff t a o s r
t t
o or an at t t o t
tt
n t u o s nto t
tt t o or
of the tip becomes lighter. If the compensation pressure is higher than the capillary
r ssur t u oo s out o t
tt
so ut on aroun t
tt
colored. Adjusting the compensation pressure prevents this from happening.
NOTE
: When adjusting the COMPENSATION PRESSURE under 3.0 PSI, you may set it in
0.01 PSI increments.
Understanding how
a ar a t on aus s t ront n o t
tt
s ou to
orr t us t o
nsat on r ssur
o rat s t r n t a ar
action and the tip size. Since the tip size is often determined by the requirement of
the application, controlling the compensation pressure becomes the main option to
nat t u ta o u a ar a t on
NOTE:
The pressure of capillary action is determined by the inner diameter of
the glass capillary where the meniscus of air/liquid interface is located. It has
nothing to do with the pipette tip size.
