WPI Nanoliter 2000, Instruction Manual

Page: 9 / 24

Nanoliter 2000
World Precision Instruments 5
Collet
Aluminum Barrel
Plunger
Set Screws
Motor Housing
Glass O-ring with Large Hole
Plastic Spacer
Sealing O-Ring
(small hole)
Clear Stop
Fig. 4 –Nanoliter 2000 (exploded view) shows the parts of the Nanoliter 2000
injector handle
Controller Buttons
Fill–
The Fill button retracts the wire plunger by one aliquot volume when
the button is depressed. A beep sounds at the extreme fill position. DIP
switch #5 (
Fig. 3, 7
) controls the rate of filling.
• #5 up = 46nL/s fill rate
• #5 down = 23nL/s fill rate
Empty–
The Empty button extends the wire plunger by one aliquot volume
when the button is depressed. A beep sounds at the fully extended
position (approximately 27mm from end of collet). The empty speed is
approximately 92nL/s.
Fast Fill and Fast Empty–
DIP switch #5 has no effect on the fast empty
mode. To empty rapidly, hold down the Empty button and touch the Fill
button one time. The empty speed increases to 230nL/s. The plunger
maintains the faster speed until the Empty button is released.
The fill mode also has a fast speed. Hold down the Fill button and touch the
Empty button one time. The fast rate varies, depending on the position of
DIP switch #5 .
• #5 up = 230nL/second
• #5 down = 92nL/second
NOTE
: When performing fast empty or fast fill, a beep sounds when the
plunger reaches the end of travel.
Inject
–The aliquot volume is selected using the DIP switches on the right
side of the control unit. Then, each time the Inject button is depressed, an
audible beep is heard and the selected volume is dispensed. See “Setting the
Aliquot Volume and Injection Speed” on page 10.