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Nanoliter 2000

World Precision Instruments

1

ABOUT THIS MANUAL

The following symbols are used in this guide:

This symbol indicates a CAUTION. Cautions warn against actions that
can cause damage to equipment. Please read these carefully.

This symbol indicates a WARNING. Warnings alert you to actions that
can cause personal injury or pose a physical threat. Please read these
carefully.

NOTES and TIPS contain helpful information.

40mm

Reversing mount 180º will
reduce manipulator-to-tip
distance by 10cm.

Mounting Rod:
8.2Ø x 50mm

13.5c

m

2.5–7.5c

m

Total volume in pipette:
1~3” = 4.9–17.4µL

Universal
Adapter for Nanoliter
Injector

Fig. 1

–Nanoliter 2000 setup. Micromanipulator is not included .

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Summary of Contents for Nanoliter 2000

Page 1: ...Nanoliter 2000 Microprocessor controlled nanoliter injection World Precision Instruments INSTRUCTION MANUAL Serial No _____________________ 092512 www wpiinc com ...

Page 2: ......

Page 3: ...troller Buttons 5 Plunger in HOME Position 6 OPERATING INSTRUCTIONS 7 Micropipette Pulling 7 Securing the Micropipette to the Injector 7 Micropipette Filling Techniques 8 Back Filling 8 Front Filling 9 Typical Technique 9 Injecting 9 Setting the Aliquot Volume and Injection Speed 10 MAINTENANCE 11 Replacing O Rings 11 Replacing the Wire Plunger 11 Cleaning Recommendations 12 OPTIONAL ACCESSORIES 1...

Page 4: ...iv World Precision Instruments ...

Page 5: ...bol indicates a WARNING Warnings alert you to actions that can cause personal injury or pose a physical threat Please read these carefully NOTES and TIPS contain helpful information 40m m Reversing mount 180º will reduce manipulator to tip distance by 10cm Mounting Rod 8 2Ø x 50mm 1 3 5 c m 2 5 7 5 c m Total volume in pipette 1 3 4 9 17 4µL Universal Adapter for Nanoliter Injector Fig 1 Nanoliter ...

Page 6: ...archers report using 30µm tips with no increase in mortality Injections of 25nL or less generally require smaller tips normally 10µm A broken tip is preferable for oocyte injection Sharp polished tips tend to deflect An excellent reference for all procedures involving the Xenopus oocyte is Kay B and H Peng Xenopus Iaevis Practical Uses in Cell and Molecular Biology Methods in Cell Biology Vol 36 A...

Page 7: ...eturns on page 19 of this manual Please contact WPI Customer Service if any parts are missing at 941 371 1003 or customerservice wpiinc com Returns Do not return any goods to WPI without obtaining prior approval RMA required and instructions from WPI s Returns Department Goods returned unauthorized by collect freight may be refused If a return shipment is necessary use the original container if po...

Page 8: ...able The injector head can be mounted in a micromanipulator WPI s M3301 model is quite suitable and frequently used The 500778 Universal Adapter for the Nanoliter Injector shaft diameter of 8 2mm is used for mounting the Nanoliter Injector to a micromanipulator The Nanoliter 2000 is comprised of a controller Fig 3 and an injector handle Fig 4 Fig 3 shows the right side of the controller where the ...

Page 9: ...from end of collet The empty speed is approximately 92nL s Fast Fill and Fast Empty DIP switch 5 has no effect on the fast empty mode To empty rapidly hold down the Empty button and touch the Fill button one time The empty speed increases to 230nL s The plunger maintains the faster speed until the Empty button is released The fill mode also has a fast speed Hold down the Fill button and touch the ...

Page 10: ...f the wire plunger should be slightly recessed from the end of the collet This prevents accidental damage to the plunger when it is not in use To reset the plunger to this position push the Fill button to retract or the Empty button to extend the plunger and simultaneously hold the Inject button When in the home position release both buttons to reset the unit ...

Page 11: ...pically backfilled with oil before attachment to the injector Silicone or mineral oil is frequently used Backfilling is facilitated by using the flexible MicroFil non metallic needle and a syringe NOTE Nanoliter 2000 will not operate properly without backfilling the micropipette Securing the Micropipette to the Injector The injector is supplied with the standard collet O ring configuration Standar...

Page 12: ... the non pulled end which has the relatively large opening that allows for rapid bulk filling Front side the pulled end of the micropipette the sharp side The filling method of choice is usually dictated by experimental requirements and is chosen at your discretion Back Filling The term back filling refers to the process of filling the pipette from the large non pulled end Back filling offers a gr...

Page 13: ...tte with a small volume for microinjection 1 Backfill the micropipette with mineral oil 2 Use the Nanoliter 2000 controller to eject the plunger from the home position This is necessary in order to allow the plunger to move backwards to create a vacuum for front filling 3 Fig 5 and Fig 6 show the assembly of the micropipette to the Nanoliter 2000 4 Once the micropipette is installed submerge the m...

Page 14: ... side of the control box Table 1 lists the DIP switch settings to use for DIP switches 1 4 to select the various aliquot volumes U up D down DIP switch 5 determines the fast injection and fill rates Up Fast 46nL s Down slow 23nL s TIP To set the DIP switches use a ball point pen or a jeweler s flat head screw driver NOTE DIP switch settings for the Nanoliter 2000 are completely different from the ...

Page 15: ...your other hand This exposes a brass fitting with two Allen screws on opposite sides of the brass fitting and the clear stop CAUTION Do NOT disassemble the Motor Housing Doing so may damage the plunger drive assembly 4 Using the 0 05 hex wrench provided with the replacement plunger loosen the two set screws NOTE The 0 035 hex wrench is for use with the Nanoliter 2010 5 Remove the plunger 6 Insert ...

Page 16: ...clave the Nanoliter 2000 The control box may be cleaned by wiping it with a clean damp cloth OPTIONAL ACCESSORIES Table 2 Accessories Part Number Description 4878 Replacement 3 5 in glass capillaries 300 4879 Replacement 7 in glass capillaries 300 300521 Spare Parts Kit includes MicroFil MF34G displacement plunger five O ring sets tools 13142 Footswitch for Nanoliter 2000 15867 Footswitch for Micr...

Page 17: ...ank Plunger doesn t retract completely Home position is set incorrectly See Plunger in HOME Position on page 6 for instruction on resetting the home position NOTE If you have a problem issue with that falls outside the definitions of this troubleshooting section contact the WPI Technical Support team at 941 371 1003 or technicalsupport wpiinc com SPECIFICATIONS The Nanoliter 2000 conforms to the f...

Page 18: ...CTION MANUAL Table 3 Micro4 Volume Setting Table Type Syringe Volume Scale Length ID mm nL step REV K Max Rate nL s Max Rate Microstep Mode nL s A 0 5µL 54 1mm 0 1085 0 0294 20 1 B 1 0µL 54 1mm 0 1534 0 0587 40 2 C 5µL 54 1mm 0 343 0 2934 202 14 D 10µL 54 1mm 0 485 0 5868 451 29 E 25µL 60mm 0 73 1 329 1022 66 F 50µL 60mm 1 03 2 646 2035 132 G 100µL 60mm 1 46 5 315 4088 265 H 250µL 60mm 2 3 13 191 ...

Page 19: ...8 9 One of these two volumes should be used to insure a proper injection By default if you leave the value on the Micro4 at 100nL 98 9nL is injected Difficulty can arise when the volume value is half or more of the next 2 3nL step For example setting the Micro4 for an injection of 10nL results in a theoretical injection volume of 9 2nL produced by 4 whole steps of the injector Five whole steps res...

Page 20: ...16 World Precision Instruments ...

Page 21: ...witch 5 9 10 DIP switches 9 DIP switch settings 10 E Empty 5 F Fast Empty 5 Fast Fill 5 filament 7 Fill 5 H home position 6 7 11 I Inject 5 injection speeds 10 L leakage 11 M Micro4 Volume 14 MicroFil 2 7 12 micromanipulator 4 micropipette 9 O oocyte 2 P parts list 2 plunger 2 11 13 R returns 3 S seal 13 softening point 7 specifications 13 T TIP10XV119 12 troubleshooting 13 U unpacking 3 W wire pl...

Page 22: ...18 World Precision Instruments DECLARATION OF CONFORMITY ...

Page 23: ...rect special or consequential arising from a failure of this product to operate in the manner desired by the user WPI shall not be liable for any damage to data or property that may be caused directly or indirectly by use of this product Claims and Returns Inspect all shipments upon receipt Missing cartons or obvious damage to cartons should be noted on the delivery receipt before signing Conceale...

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