INV-101
18
WORLD PRECISION INSTRUMENTS
4. View the phase ring through the phase telescope.
5. Adjust the focus on the phase telescope for a sharp image of the phase ring. The
phase telescope focus is adjusted by screwing it in or out (
Fig. 62
).
Fig. 62
–Focus adjustment
6. Slide the phase aperture fame into position with the 10X/20X condenser annulus in
the condenser light path. (
Fig. 63
)
NOTE
: If you are using a 40X phase contrast objective (not included), use the 40X
condenser annulus on the phase aperture frame.
Fig. 63
–Plate position by phase
7. You will see an overlap of the condenser annulus and phase ring patterns through
the phase telescope (
Fig. 64
). To optimize the phase contrast image, the condenser
annulus must be concentrically aligned with the phase ring. The two rings can be
seen through the telescope. See
Fig. 60
, page 17.
Fig. 64
–Aperature disc seen through the telescope
