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World Precision Instruments
2. Set the vaporizer concentration to 5% until the animals become immobile.
3. Then, reduce the vaporizer setting to the species-appropriate level. Set the
Isoflurane vaporizer dial to:
•
Mice: 1.5%
•
Rats <300gms: 2%
•
Rats >300gms: 2 – 3%
•
Guinea Pigs: 5%
NOTE
: Guinea pigs should be anesthetized at 5%. Reduce the vaporizer setting to 3%
before transferring the animal to the surgical bed.
NOTE
: When performing surgery longer than one hour, it may be necessary to reduce
the Isoflurane vaporizer dial setting by 0.5-1.0% to prevent the animals from being
exposed to too much gas.
4. To be sure of complete anesthesia, animals should remain in the induction
chamber for 3–5 minutes after initial induction before moving them to the
surgical breathing unit. Animals in the induction chamber will remain anesthetized
for as long as the gas flow continues.
MAINTENANCE
1. Once animals are ready to be moved to the breathing unit, be sure that the
desired breathing units are connected to the manifold.
2. Reduce the secondary flowmeter setting to zero, then press the oxygen flush
button for 5-10 seconds to flush anesthetic from the chamber.
WARNING: ONLY USE THE CHAMBER FLUSH WITH CHAMBERS
SPECIFICALLY DESIGNED FOR THIS PURPOSE. CHAMBER MUST HAVE A
POSITIVELY SEALING GASKET BETWEEN THE LID AND BODY OF THE
CHAMBER.
3. Quickly open the lid, remove the desired animals and place them on the surgery
surface.
4. Remove the black plug from the nosecone and gently slide the animal into the
nosecone. Be sure that the animal’s face has a tight fit inside the nosecone.
5. If animals are to remain in the induction chamber, open the secondary flowmeter
to half of the total inlet flow (For example, 0.5 liters/min. for mice).
6. If no animals remain in the induction chamber, be sure that the secondary
flowmeter is turned fully clockwise to the OFF position.
NOTE: Use only the EZ-104 Versaflex Breathing Device for animals weighing more
than 500g. The other units cannot handle the tidal volume of larger animals.
Summary of Contents for EZ Anesthesia
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