Setting Calibration Parameters
64
4
•
Specify
Scanning
to calibrate only for scanning acquisitions. This limits acquisitions
to the same mass range and scan speed you specified in the calibration.
•
Specify
Scan Speed Compensation
to calibrate only for scanning acquisitions over
the same mass range and at the same scan speed you specified in the calibration.
Thus to correctly calibrate for scan speed compensation, you must also perform a
scanning calibration.
•
Specify both
Static
and
Scan Speed Compensation
to calibrate for acquisitions
where the quadrupole “parks” at a single mass. Also, provided you specify the same
scan speed, this calibrates for scanning acquisitions whose mass ranges lie within
the scanning calibration’s mass ranges. Accordingly, when you specify a 2-second
scan (400 amu/sec) calibration from 100 to 900 m/z, the instrument can acquire data
from 100 to 500 amu for a 1-second scan (also 400 amu/sec) and maintain correct
mass assignment. In this case the static calibration would determine the acquisition’s
start mass and the scanning calibration the mass assignment and scan range.
•
Specify both
Scanning Calibration
and
Scan Speed Compensation
to calibrate for
scanning acquisitions over the mass range you specify for the calibration. You can,
however, change the scan speed, provided it remains within the scan speeds you
specified in the two calibrations. In this case do not change the mass range, because
no static calibration exists to locate the start mass.
•
Specify all three types of calibration,
Static
,
Scanning
, and
Scan Speed
Compensation
, to allow all types of acquisitions, provided the mass range and scan
speed fall between the lower and upper limits for scanning calibration and scan speed
compensation.
4.2.7 Calibration Acquisition Dialog Box
Specify mass ranges, scan speeds and acquisition mode in this dialog box, which, when
you first open it, contains default parameter values for the specified reference file. These
values represent the scan range and speed limits of the parameters and instrument.
Note:
For improved calibration performance, Waters suggests you adopt the parameter
values in
Click
Acquisition Parameters
in the Automatic Calibration dialog box (
) to open
the Calibration Acquisition Setup dialog box (
Summary of Contents for Micromass ZQ 2000
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