Waltron AQUALERT Aqualyzer 3051, Instruction Manual, Page: 19 / 70

Introduction
18
Waltron User Manual
3051 Hardness Analyzer
2.4
ANALYSIS CYCLE
2.4.1
PROGRAMMING COMMANDS
An analysis time program in the Waltron 3051 Hardness Analyzer has the following structure:
1. After rinsing the reaction colorimetric cell and taking a sample, one or more
miscellaneous reagents, like buffer or masking agent, may be added after which a first
measurement takes place (reference); in this way interfering factors are eliminated such
as sample color or turbidity, miscellaneous reagents color and refractive index variations.
2. The second measurement (reading sensor) takes place after addition of color reagent and
color development, which may take a certain time period, depending on the color
reaction.
Table 2.1: The run sequence of colorimetric determination.
Conditioning, rinsing and sampling
Drain, rinse and sample functions
First the cuvette is drained and rinsed (this can also be
programmed at the end of the run). In this way the hydraulic
line and the colorimetric cell is rinsed as well prior to the
actual sample taking. Next a sample is taken.
Addition of reagent(s)
Add reag function
Depending on the method one or more reagents may be
added before reference.
Mixing and wait
Mix and wait functions
The mixing pump is activated and the liquid is pumped from
the lower part to the upper part of the colorimetric cell. The
waiting time is programmed in order to eliminate bubbles,
suspensions.
First measurement
Reference function
Regulation of the light intensity to a programmed value, in
order to start from a fixed reference point and eliminate
interfering factors (sample turbidity, color).
Addition of color reagent(s)
Add reag function
Depending on the method one or more reagents may be
added for the color development.
Mixing and wait
Mix and wait functions
The mixing pump is activated and the liquid is pumped from
the lower part to the upper part of the colorimetric cell; in
this way reagent(s) is mixed. The waiting time is
programmed in order to complete the colorimetric reaction.
Reading, absorbance and
concentration calculation
Read sensor, absorbance, calculation
Reading of the light intensity after the colorimetric reaction,
calculation of the absorbance and of the concentration.
Drain, conditioning, rinsing, sampling
Drain, rinse and sample functions
Drain and rinse of the hydraulic line and the colorimetric
cell.
Waiting time (analysis frequency)
Wait function
The wait function allows the user to set the analysis
frequency.