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7     MAINTENANCE & SERVICING

When the pipette is not in use it should be stored in an upright 
position. The pipette should be inspected prior to use each day 
for  any  dust  or  contamination  on  outside  surfaces.  Special 
attention should be given to the tip cone. No solvent other than 
isopropanol should be used to clean the pipette. If the pipette is 
used daily, an internal parts inspection should be performed 
every three (3) months.

7.1   DISASSEMBLY

The  servicing  procedure  starts  with  the  disassembly  of  the 
pipette.  Please  refer  to  the  spare  parts  lists  for  better 
understanding of the components.

7.2   DISASSEMBLING THE LOWER PART

1.  Press tip ejector pusher completely down and hold.
2.   Pull down the tip ejector and release the tip ejector
 

pusher.

- 11 -

• 

Calculate the inaccuracy and imprecision for all three

 

volumes as per EN ISO 8655-6 standards on the basis

 

of the following calculation

Mean Volume

Mean Weight

Balance Reading

number of reading
Conversion factor
[example Z=1.0040 µl/mg at 25°c and 1013 hPa]

Mean Value
Particular volume at which readings are taken

S  = Standard Deviation

n  = number of readings  

S=

V  = Mean Value

i=1

n

∑ ( Vi - V)

2

n - 1

CV%  

=   100 · S 

_

V

Compare the results to the 
limits in the earlier tables 
(Page

)

# 4, 5

6.3.1   CONVERSION OF WEIGHT READINGS TO VOLUME

6.3.2   CALCULATION FOR IN-ACCURACY (SYSTEMATIC ERROR)

6.3.3   CALCULATION FOR IMPRECISION (RANDOM ERROR)

Summary of Contents for VWRA613-6688

Page 1: ...Precise High Performance PHP Pipette...

Page 2: ......

Page 3: ...rocedure to check calibration 10 6 3 1 Conversion of weight readings to volume 11 6 3 2 Calculation for in accuracy systematic error 11 6 3 3 Calculation for imprecision random error 11 7 Maintenance...

Page 4: ...under the scope of in vitro diagnostics and can be used as a diagnostic medical device in related applications Thereby it ful ls the relevant demands of the directive 98 79 EC of the European Parliame...

Page 5: ...2 SINGLE CHANNEL VARIABLE VOLUME Volume adjustment knob Tip ejector Volume lock Tip ejector pusher Volume range Tip cone Coupler...

Page 6: ...3 MULTICHANNEL VARIABLE VOLUME Volume adjustment knob Volume lock Tip ejector pusher Volume range Coupler Tip cone Manifold...

Page 7: ...VWRA613 6692 100 1000 l 1 100 500 1000 3 1 0 6 0 6 0 2 0 2 VWRA613 6694 2 20 l 0 02 2 10 20 3 1 2 0 9 1 5 0 6 0 3 VWRA613 6690 VWRA613 6693 4 2 FIXED VOLUME PIPETTE RANGE VWRA613 6697 VWRA613 6698 VWR...

Page 8: ...02 0 02 0 1 10 50 100 20 100 200 30 150 300 1 5 10 2 10 20 5 25 50 3 1 0 8 5 1 0 7 3 1 0 6 8 4 2 7 3 2 3 1 5 1 2 1 0 7 VWRA613 6713 VWRA613 6719 Delivery volume is clearly indicated in the volume disp...

Page 9: ...osition and keep the tips at a constant depth below the surface of the liquid Pre rinse the pipette tip before aspirating the sample by lling and emptying the pipette tip ve 5 times This is important...

Page 10: ...the volume lock lever to the unlock position so that the volume can be adjusted to the desired setpoint within the permitted volume range Refer following images After pipetting acids or other corrosi...

Page 11: ...of speci cation The tip angle is also important the pipette should always be used in a position within 20 angle of vertical See gure below 5 4 OPTIMUM IMMERSION DEPTHS Volume Immersion depth 0 1 l 1...

Page 12: ...at have high viscosity or a tendency to foam easily This technique is also recommended for dispensing very small volumes 1 Press the operating knob until the second stop 2 Dip the tip under the surfac...

Page 13: ...e of water pipette and air between 15 C to 30 C The relative humidity must be above 50 especially with volumes under 50 l the air humidity should be as high as possible to reduce the e ect of evaporat...

Page 14: ...e components 7 2 DISASSEMBLING THE LOWER PART 1 Press tip ejector pusher completely down and hold 2 Pull down the tip ejector and release the tip ejector pusher 11 Calculate the inaccuracy and impreci...

Page 15: ...tissue Allow them to dry Check the tip cone for foreign articles and remove if any Grease the cleaned parts with the approved lubricant provided with each pipette 7 2 1 DISASSEMBLING THE SINGLE CHANNE...

Page 16: ...Slightly push the spring and pull up tip cone to remove it from the lower rail image 3 2 Carefully release the piston from the upper rail and move upward to remove it image 4 7 5 3 REMOVING THE CHANNE...

Page 17: ...0 5 10 l Fix volume 10 l Group 1 Variable volume 0 1 2 5 l Fix volume 2 5 l 7 6 LIST OF SPARE PARTS SINGLE CHANNEL PIPETTE DP 037 DP 0262 DP 0159 SP 0158 DP 022 DP 022 SP 0160 DP 0159 DP 0261 DP 036 D...

Page 18: ...Group 3 Variable volume 2 20 l Fix volume 20 l Group 4 Variable volume 5 50 l Fix volume 25 l 50 l DP 029 DP 0269 DP 0263 DP 038 SP 0152 DP 023 DP 033 DP 0270 DP 0264 DP 039 DP 0361 DP 0152 DP 0160 DP...

Page 19: ...ariable volume 10 100 l Fix volume 100 l Group 6 Variable volume 20 200 l Fix volume 200 l DP 035 DP 0270 DP 0363 DP 0154 DP 0162 DP 026 DP 041 DP 0266 DP 034 DP 0270 DP 0362 DP 0153 DP 0161 DP 025 DP...

Page 20: ...17 Group 7 Variable volume 100 1000 l Fix volume 250 l 500 l 1000 l DP 34 DP 271 DP 042 DP 265 DP 0155 DP 0163 DP 027...

Page 21: ...ariable volume 0 5 5ml Fix volume 2ml 5ml Group 9 Variable volume 1 10ml Fix volume 10ml DP 020 DP 0268 DP 0272 SP 0168 SP 0060 RP 0156 SP 0110 DP 047 DP 021 DP 0268 DP 0273 SP 0168 SP 0044 SP 0162 SP...

Page 22: ...M 277 E DPM 364 DPM 364 DPM 365 DPM 366 DPM 367 DPM 368 F SPM 152 SPM 154 SPM 101 SPM 155 SPM 150 SPM 093 G DPM 278 DPM 278 DPM 278 DPM 278 DPM 278 DPM 278 H DPM 128 DPM 129 DPM 130 DPM 131 DPM 132 DP...

Page 23: ...e is inaccurate Inaccurate dispensing with certain liquids Worn o ring or seal Worn o ring or seal Tip cone is loose Damaged tip cone Improper assembly Tip incorrectly attached Calibration altered Cal...

Page 24: ...Avantor Performance Materials India Limited 17th Floor Building No 5 Tower C DLF Cyber City Phase III Gurgaon 122002 Haryana...

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