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• 

To decrease the volume, turn the

 

volume setting knob clockwise.

• 

To increase the volume, turn the

 

volume setting knob counterclock-

 

wise.

• 

Do make sure that the desired

 

delivery volume is set in-line with

 

pointer. 

• 

Turn the volume lock to “lock” the

 

volume setting, preventing any

 

accidental change in the volume

 

during pipetting. 

 

For this pipette the delivery volume

 

of liquid is set using the digital

 

display. A pointer is used to set

 

exact or intermediate volumes

 

using the scale on the last wheel of

 

digital display (refer point # 4.6)

 

The  Locking  mechanism  ensures  that  the  volume 

adjustment  knob  remains  at  the  setpoint  while  aspirating  or 
dispensing  sample  liquids.  Any  effort  to  rotate  the  volume 
adjustment  knob  with  the  locking  mechanism  engaged  will 
damage the locking mechanism and void the warranty.

5.2   SETTING THE VOLUME

• 

To set the volume turn the volume lock lever to the “unlock”

 

position so that the volume can be adjusted to the desired

 

setpoint within the permitted volume range. Refer 

 

following images

• 

After pipetting acids or other corrosive liquids that emit

 

vapors, remove the tip-cone and rinse the piston, O-ring

 

and seal with distilled water.

• 

Do not pipette liquids having temperatures above 70°C.

 

(Not recommendable)

• 

Make sure that liquids never enter the tip-cone. To

 

prevent this:

 

Avoid laying the pipette horizontally when tip is filled

 

with sample. As liquid may enter in and contaminate the

 

sample during next pipetting cycle.

  

When there is liquid in the tip, press and release the

 

volume adjustment knob slowly and smoothly. 

 

Never turn the pipette upside down.

• 

Always store the pipette without tip on a shelf mounting 

 

stand supplied with or a pipette carousel stand. 

• 

Highly recommended to calibrate the pipette once in

 

every 3-6 months (depending on the sensitivity of

 

usage) for better performance. The calibration must be

 

carried by gravimetric method in accordance of DIN ISO

 

8655-6. 

Summary of Contents for VWRA613-6688

Page 1: ...Precise High Performance PHP Pipette...

Page 2: ......

Page 3: ...rocedure to check calibration 10 6 3 1 Conversion of weight readings to volume 11 6 3 2 Calculation for in accuracy systematic error 11 6 3 3 Calculation for imprecision random error 11 7 Maintenance...

Page 4: ...under the scope of in vitro diagnostics and can be used as a diagnostic medical device in related applications Thereby it ful ls the relevant demands of the directive 98 79 EC of the European Parliame...

Page 5: ...2 SINGLE CHANNEL VARIABLE VOLUME Volume adjustment knob Tip ejector Volume lock Tip ejector pusher Volume range Tip cone Coupler...

Page 6: ...3 MULTICHANNEL VARIABLE VOLUME Volume adjustment knob Volume lock Tip ejector pusher Volume range Coupler Tip cone Manifold...

Page 7: ...VWRA613 6692 100 1000 l 1 100 500 1000 3 1 0 6 0 6 0 2 0 2 VWRA613 6694 2 20 l 0 02 2 10 20 3 1 2 0 9 1 5 0 6 0 3 VWRA613 6690 VWRA613 6693 4 2 FIXED VOLUME PIPETTE RANGE VWRA613 6697 VWRA613 6698 VWR...

Page 8: ...02 0 02 0 1 10 50 100 20 100 200 30 150 300 1 5 10 2 10 20 5 25 50 3 1 0 8 5 1 0 7 3 1 0 6 8 4 2 7 3 2 3 1 5 1 2 1 0 7 VWRA613 6713 VWRA613 6719 Delivery volume is clearly indicated in the volume disp...

Page 9: ...osition and keep the tips at a constant depth below the surface of the liquid Pre rinse the pipette tip before aspirating the sample by lling and emptying the pipette tip ve 5 times This is important...

Page 10: ...the volume lock lever to the unlock position so that the volume can be adjusted to the desired setpoint within the permitted volume range Refer following images After pipetting acids or other corrosi...

Page 11: ...of speci cation The tip angle is also important the pipette should always be used in a position within 20 angle of vertical See gure below 5 4 OPTIMUM IMMERSION DEPTHS Volume Immersion depth 0 1 l 1...

Page 12: ...at have high viscosity or a tendency to foam easily This technique is also recommended for dispensing very small volumes 1 Press the operating knob until the second stop 2 Dip the tip under the surfac...

Page 13: ...e of water pipette and air between 15 C to 30 C The relative humidity must be above 50 especially with volumes under 50 l the air humidity should be as high as possible to reduce the e ect of evaporat...

Page 14: ...e components 7 2 DISASSEMBLING THE LOWER PART 1 Press tip ejector pusher completely down and hold 2 Pull down the tip ejector and release the tip ejector pusher 11 Calculate the inaccuracy and impreci...

Page 15: ...tissue Allow them to dry Check the tip cone for foreign articles and remove if any Grease the cleaned parts with the approved lubricant provided with each pipette 7 2 1 DISASSEMBLING THE SINGLE CHANNE...

Page 16: ...Slightly push the spring and pull up tip cone to remove it from the lower rail image 3 2 Carefully release the piston from the upper rail and move upward to remove it image 4 7 5 3 REMOVING THE CHANNE...

Page 17: ...0 5 10 l Fix volume 10 l Group 1 Variable volume 0 1 2 5 l Fix volume 2 5 l 7 6 LIST OF SPARE PARTS SINGLE CHANNEL PIPETTE DP 037 DP 0262 DP 0159 SP 0158 DP 022 DP 022 SP 0160 DP 0159 DP 0261 DP 036 D...

Page 18: ...Group 3 Variable volume 2 20 l Fix volume 20 l Group 4 Variable volume 5 50 l Fix volume 25 l 50 l DP 029 DP 0269 DP 0263 DP 038 SP 0152 DP 023 DP 033 DP 0270 DP 0264 DP 039 DP 0361 DP 0152 DP 0160 DP...

Page 19: ...ariable volume 10 100 l Fix volume 100 l Group 6 Variable volume 20 200 l Fix volume 200 l DP 035 DP 0270 DP 0363 DP 0154 DP 0162 DP 026 DP 041 DP 0266 DP 034 DP 0270 DP 0362 DP 0153 DP 0161 DP 025 DP...

Page 20: ...17 Group 7 Variable volume 100 1000 l Fix volume 250 l 500 l 1000 l DP 34 DP 271 DP 042 DP 265 DP 0155 DP 0163 DP 027...

Page 21: ...ariable volume 0 5 5ml Fix volume 2ml 5ml Group 9 Variable volume 1 10ml Fix volume 10ml DP 020 DP 0268 DP 0272 SP 0168 SP 0060 RP 0156 SP 0110 DP 047 DP 021 DP 0268 DP 0273 SP 0168 SP 0044 SP 0162 SP...

Page 22: ...M 277 E DPM 364 DPM 364 DPM 365 DPM 366 DPM 367 DPM 368 F SPM 152 SPM 154 SPM 101 SPM 155 SPM 150 SPM 093 G DPM 278 DPM 278 DPM 278 DPM 278 DPM 278 DPM 278 H DPM 128 DPM 129 DPM 130 DPM 131 DPM 132 DP...

Page 23: ...e is inaccurate Inaccurate dispensing with certain liquids Worn o ring or seal Worn o ring or seal Tip cone is loose Damaged tip cone Improper assembly Tip incorrectly attached Calibration altered Cal...

Page 24: ...Avantor Performance Materials India Limited 17th Floor Building No 5 Tower C DLF Cyber City Phase III Gurgaon 122002 Haryana...

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