VWR International 634-6002, Instruction Manual

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Appendix 1
Methods of Quantitative Analysis
Single Wavelength Method
：
Abs.=A1
Double Wavelengths Method
：
Abs.=|m*A1-n*A2|
Three Wavelengths Method
：
Abs.=A1-(W1-W2)*(A2-A3)/(W2-W3)-A3
Appendix 2
Methods of DNA/Protein Analysis
Method 1
：
CDNA=(A1-Aref)*f1-(A2-Aref)*f2
CProtein= (A2-Aref)*f3-(A1-Aref)*f4
Ratio=(A1-Aref)/ (A2-Aref)
A1=A260nm
，
A2=A280nm
，
Aref=A320nm (Optional)
f1=62.9
，
f2=36.0
，
f3=1552
，
f4=757.3
Method 2
：
CDNA=(A1-Aref)*f1-(A2-Aref)*f2
CProtein= (A2-Aref)*f3-(A1-Aref)*f4
Ratio=(A1-Aref)/ (A2-Aref)
A1=A260nm
，
A2=A230nm
，
Aref=A320nm (Optional)
f1=49.1
，
f2=3.48
，
f3=183
，
f4=75.8