V&P Scientific, Inc.
7
Technical Note 396C
USING A 300 ML SpinVessel
®
SYSTEM
The optimal stirring mode is dependent upon the application and needs to be empirically
determined.
Factors to consider in determining optimal 300 mL SpinVessel® operation are the density
of the particulates, their size and shape, as well as the volume and viscosity of the liquid. Start with 50
RPM and 2.1 rotations before reversing direction, then work up to the appropriate RPMs and number of
rotations for the
particulate’s density, fragility, and solution viscosity. We have found that smaller
circumference SpinVessels® require higher RPMs than larger circumference SpinVessels do, because the
critical factor is the linear speed generated at the circumference not the RPMs. See page 501 of our
for a discussion of this phenomenon.
The following are settings to use with an aqueous solution when first testing the VP 418SV-300FB-CC
SpinVessel®:
Speed: 50 RPM
Rotate: 2.1 Pause: 200 milliseconds
Ramp: 10
It is always a good idea to practice with water in your SpinVessel to determine safe RPM, Rotation, Pause
and Ramp numbers before you use expensive reagents.
Note that, if Ramp is set to 0, liquid will splash out of the Spin Vessel.
The VP 418SV-1-300FB-CC SpinVessel® is designed to be used only with a
VP 830SV-300FB SpinVessels®
tube. Please contact V&P Scientific for pricing.
See below for an example of low versus high RPM for a solution of yeast cells in a large circumference 6
liter SpinVessel®. Note: That when given a longer mixing time even the slower speed (20 RPM i.e.,
–
more
gentle mixing) was still able to suspend the yeast cells.
0.00
0.20
0.40
0.60
0.80
1.00
1.20
2
5
9
14 18 24 28 33 37 40 44 60 90 120 230 480 720
A
bso
rb
anc
e
Time (Seconds)
Yeast Resuspension with 6L SpinVessel®
20 RPM vs 100 RPM
20 RPM, 4 rotations, 500 pause
100 RPM, 4 rotations, 500 pause
