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APPLICATION NOTE

T2O-AN-C270

NITRITE (C270) METHOD

The following application note explains the procedure for the detection of NITRITE (C270) using the Comparator.

Equipment:

•

Comparator

•

2 x 10ml cells

•

Stirring rod

•

Nitrite LR tablet

Safety:

•

Consult the safety data sheet for all of the reagents before use. Even if you have used comparator
reagents before, the formulation may have changed.

Getting started:

Fig 1.0 Comparator Disc

Fig 1.1 Reference cell

(Disc shown is for illustration purposes only)

COMPARATOR

Summary of Contents for AquaSafe WSL25 Plus

Page 1: ...AquaSafe WSL25 Plus Water Safety Laboratory Instruction Manual...

Page 2: ...Contents Section 3 Single Incubator Instructions Section 4 Membrane Filtration Instructions Section 5 Turbidity Tube Instructions Section 6 Comparator Instructions Section 7 Pocket pH Tester Instruct...

Page 3: ...comprises the following Integrated single chamber digital incubator complete with all accessories and consumables for carrying out 300 tests for Faecal and Total Coliforms Comparator complete with ac...

Page 4: ...Section 2 Kit Contents 2 1 3 4 Under tray 8 9 12 13 14 15 16 17 18 19 Under charger 10 11 6 5 7 a a a a b b c d e f g h i k j...

Page 5: ...on Tablet Bags Whirl Pak 14 1 Comparator Chlorine Disc 14 1 Comparator Ammonia Disc 14 1 Comparator Nitrate Disc 14 1 Comparator Nitrite Disc 15 4 Comparator Cells 16 1 Nitrate Test Tube 17 1 Test Tub...

Page 6: ...ck 3 Chlorine Free Total Reagents 100 Pack 3 Ammonia Reagents 100 Pack 3 Nitrate Reagents 100 Pack 1 pH 4 01 Calibration Solution 1 pH 7 00 Calibration Solution 1 EC 1413 S Calibration Solution 2 Ster...

Page 7: ...Section 3 AquaSafe Micro biological Incubator Instruction Manual...

Page 8: ...5 3 3 2 Start Screen Figure 1 1 16 3 3 3 37 C Program Fig 1 2 17 3 3 4 44 C Program Fig1 3 18 3 3 5 User defined Program Fig 1 4 19 3 3 6 Resetting the timer Fig 1 5 19 3 3 7 Incubation cycle time set...

Page 9: ...with the supplied 54mm x 3 5mm aluminium petri dishes which are suitable for 47mm membrane filter pads but the incubator can also be used with 55mm pre prepared plastic petri dishes The incubator is...

Page 10: ...t become depressed during transit covering the recessed plate with rigid card should achieve this 2 1 Docking Station The Incubator is mounted on a docking station built into the carry case which inco...

Page 11: ...if absolutely necessary 2 3 Battery Charging It is recommended to charge the battery fully prior to use To do this remove the power supply unit from the kit and connect it to the charging connection o...

Page 12: ...used cable available separately Note The vehicle cigarette lighter charging lead is a specialist product and as such only a genuine Trace2o replacement should be used Replacements are available from...

Page 13: ...sting to the left and then removing the top To re fit align the arrow with either of the two slots in the top of the rack push down and twist lock into place The incubator has a power connection socke...

Page 14: ...ilure conditions If Fig 1 0 is displayed and the incubator was in mode 2 then the previous incubation cycle can be restarted from where it was left by pressing restart This will be discussed later The...

Page 15: ...sequence 20 9 C 24 00 OFF MEM 10 5v Select Option Restart New Run 20 9 C 24 00 37 MEM 10 5v 20 9 C 24 00 44 MEM 10 5v 20 9 C 24 00 U MEM 10 5v 20 9 C 24 00 New SEL 10 5v 20 9 C 24 00 Cal ADJ 10 5v 20...

Page 16: ...e 1 1 This screen shows the current status of the incubator In the state shown the incubator is in idle mode and not running Current Temperature Time Remaining Incubator Status Battery Voltage Mode 20...

Page 17: ...hold the middle switch again for 5 seconds The display will return to the one shown in Fig 1 2 Once the chamber has reached the pre set temperature the timer will start to countdown If for any reason...

Page 18: ...is described in section 3 3 7 Pressing and holding the middle switch for 5 seconds will start the incubation cycle and the screen will change to the following The asterisk preceding the temperature wi...

Page 19: ...middle switch again for 5 seconds The display will return to the one shown in Fig 1 2 3 3 6 Resetting the timer Fig 1 5 In this mode pressing the middle switch will display the following screen which...

Page 20: ...middle switch to save the settings The display will return to the one shown in Fig 1 1 3 3 8 User defined temperature setting Fig1 7 In this mode the user defined incubation temperature can be adjust...

Page 21: ...Compare the displayed temperature on the incubator screen to that shown on the thermometer If there is a difference 0 2 C the calibration should be adjusted as follows Exit the run mode and step thro...

Page 22: ...et conditions and must not be submersed in water The incubator chamber is sealed at the bottom so spills will not affect the unit but should be cleaned as soon as possible The Aluminium chamber can be...

Page 23: ...e rechargeable lead acid batteries may be necessary If so these must be replaced with batteries of the same type as specified in section 5 1 and replaced as a pair Should any fault occur please contac...

Page 24: ...d Assistance Trace2o hope that the AquaSafe incubator will give many years of trouble free operation but in the event of a technical problem occurring the AquaSafe incubator is covered by Trace2o Ltd...

Page 25: ...Section 4 AquaSafe Membrane Filtration Instruction Manual...

Page 26: ...29 SECTION 3 PREPARING BACTERIOLOGICAL MEDIA IN THE FIELD 30 SECTION 4 SAMPLING 31 SECTION 5 USE OF BACTERIOLOGICAL MEDIA 33 SECTION 6 ASEPTIC PROCEDURES 34 SECTION 7 PROCESSING SAMPLES FOR COLIFORM A...

Page 27: ...FILTRATION APPARATUS 1 Hand Vacuum Pump 2 Filtrate Flask Waste Beaker 3 Sampling Cup and cable 4 Graduated Aluminium Funnel 5 Membrane Support Holder 6 Sealing Gaskets 7 Glass Sintered Disc Membrane...

Page 28: ...and press into place Place the sintered disc smooth side facing upwards into the centre of the gasket Push the other two gaskets into place around the sintered support disc The graduated funnel screw...

Page 29: ...cleansing agent pour the prepared medium carefully into the bottles Replace bottle lids but leave them slightly loose do not fully tighten Sterilise in an autoclave at 121 C for 10 minutes or place b...

Page 30: ...nge to green to indicate moisture penetration Tear open the sachet and remove the silica gel with the forceps If the silica gel is green discard the sachet without using Open a bottle of Sterilised wa...

Page 31: ...on bottles contain dechlorination tablets N B If the sample is analysed immediately on site then it is not necessary to dechlorinate the water Dechlorination The dechlorination tablets are pre placed...

Page 32: ...ntil the water cools to its original temperature Fill the prepared bacteriological sampling bottle leave a slight air gap at the top and seal the lid tightly Dip Sample Sterilise the sampling cup by i...

Page 33: ...in a water bath for 15 minutes Clean empty media bottles thoroughly before re use Any residues should be washed out with hot water cleaned with a little detergent a small brush can be used if required...

Page 34: ...ignite the methanol TAKE CARE AROUND THE NAKED FLAME Allow the methanol to burn for a few seconds Whilst the methanol is still burning invert the filtration unit and carefully place inside the sample...

Page 35: ...is necessary to dispense pads in the field great care must be taken not to contaminate either the pad dispenser or the cartridge As soon as a cartridge is finished a new one should be attached to the...

Page 36: ...e the pad with prepared broth Loosen the graduated filter funnel and remove from the base support Sterilise the forceps and allow to cool Using these forceps place a sterile membrane onto the glass me...

Page 37: ...unnel up to the 100 ml graduation Connect the hand vacuum pump to the filtration unit base ensuring the arrow is orientated to the direction of desired air flow and pump in a controlled fashion to suc...

Page 38: ...he sample number place date time etc Place the petri dish into the rack Repeat the process for all the samples and then place the filled rack into the incubator It is important to note that when the l...

Page 39: ...hin a few minutes of removing from the incubator as the colours are liable to change on cooling and standing Ignore those colonies that are not yellow e g pink transparent colonies Once the number of...

Page 40: ...s 10 faecal coliforms per 100 mL action is urgently required Raw Waters For source waters and partially treated waters including those which are ground water derived it can be useful to adjust the sam...

Page 41: ...Section 5 AquaSafe Turbidity Tube Instruction Manual...

Page 42: ...turbidity tube from their position in the foam recess at the front of the AquaSafe Kit Align the two halves of the turbidity tube so that the graduations are easily visible then push together the gre...

Page 43: ...tively fill the tube then slowly pour small portions away until the moment that the Secchi marker becomes visible from the top of the tube Hold the tube vertically and identify the water level The tur...

Page 44: ...Section 6 AquaSafe Comparator Instruction Manual...

Page 45: ...ator Equipment Comparator 2 x 10ml cells Stirring rod Ammonia Nos 1 2 tablets Safety Consult the safety data sheet for all of the reagents before use Even if you have used comparator reagents before t...

Page 46: ...No 1 tablet straight from the foil to the water sample in the second cell Crush the tablet using a clean stirring rod until no large pieces are visible Add one Ammonia No 2 tablet straight from the fo...

Page 47: ...clean The tablets must be added in the correct sequence Ammonia No 1 tablet will only dissolve fully after Ammonia No 2 tablet is added The tablets should be added direct from the foil avoiding contac...

Page 48: ...rator Equipment Comparator 2 x 10ml cells Stirring rod DPD No 1 DPD No 3 Tablets Safety Consult the safety data sheet for all of the reagents before use Even if you have used comparator reagents befor...

Page 49: ...parator as shown in fig 1 1 This is the reference cell Free Chlorine Sample preparation Add one DPD No 1 tablet straight from the foil to the water sample in the second cell Crush the tablet using a c...

Page 50: ...reading on the disc Total Chlorine Sample preparation Remove the cell from the right side of the comparator Remove the lid and add one DPD No 3 tablet straight from the foil to the water sample Crush...

Page 51: ...the reading on the disc Combined Chlorine mg L ppm Total Chlorine mg L Free Chlorine mg L Notes For best results rinse vials thoroughly between with deionised water Ensure that the outside of the via...

Page 52: ...2 x 10ml cells Stirring rod Nitrate test tube Nitrate test powder Nitrate test tablet Nitrite LR tablet Safety Consult the safety data sheet for all of the reagents before use Even if you have used c...

Page 53: ...one Nitrate Test tablet straight from the foil to the nitrate test tube Do not crush the tablet Refit the cap and swirl vigorously for 1 minute Stand the tube upright allow contents to settle then inv...

Page 54: ...foil avoiding contact with hands or surfaces Nitrite present in the sample will also react leading to a higher result To correct carry out a nitrite test C270 on the sample and subtract the result Ni...

Page 55: ...mparator Equipment Comparator 2 x 10ml cells Stirring rod Nitrite LR tablet Safety Consult the safety data sheet for all of the reagents before use Even if you have used comparator reagents before the...

Page 56: ...n in fig 1 1 This is the reference cell Sample preparation Add one Nitrite LR tablet straight from the foil to the water sample in the second cell Crush the tablet using a clean stirring rod until no...

Page 57: ...est results rinse vials thoroughly between with deionised water Ensure that the outside of the vials are clean The tablets should be added direct from the foil avoiding contact with hands or surfaces...

Page 58: ...Section 7 HydroLite HL101 Pocket pH Tester Instruction Manual...

Page 59: ...r is fully functional the tip of the probe must be submerged in liquid during when not in use If left to dry out the electrode could be damaged and therefore affect the testing Trace2o uses a potassiu...

Page 60: ...old Power the unit ON OFF Freezes the currently displayed value for recording press the key again to resume measuring When in calibration mode exits calibration and returns to measurement mode Cal Pre...

Page 61: ...compensation is enabled Prior to Use Remove the electrode protective cap from the unit If the membrane on the electrode dries out soak the electrode in 3M KCL solution or tap water for at least 15 mi...

Page 62: ...able reading Disable Auto Off Automatically turn off the unit Disable Reset Restore factory settings Disable Changing the default parameters 1 Press and hold the CAL key for 3 seconds to enter the set...

Page 63: ...abled If the auto hold function is enabled the unit will automatically sense a stable endpoint reading and freeze the reading in the display If you disable this function the unit allows user to freeze...

Page 64: ...es and selected parameters will be reset 10 Press CAL key to enable or disable the reset function Press ENTER key to confirm the unit returns to measurement mode EXIT THE SETUP MENU During the setup m...

Page 65: ...r the electrode is replaced To ensure optimum accuracy regular calibration is recommended Do not reuse calibration solution after calibration contaminants in solution will affect the calibration and e...

Page 66: ...stabilize the display shows electrode slope and END 2 point calibration is completed 3 POINT CALIBRATION not required for normal operation 3 1 Ensure that 3 point calibration is selected in the setup...

Page 67: ...ir the solution gently 3 6 Press ENTER key to confirm Wait for the measured value to stabilise the display shows electrode slope and END Calibration is completed EXIT THE CALIBRATION During the calibr...

Page 68: ...Press CAL key to enter the temperature calibration mode 4 Press CAL key again to set temperature value Resolution 0 5o C 5 Press ENTER key to confirm the display shows next option 6 Press ON OFF key...

Page 69: ...the pH electrode membrane wet Ensure that the electrode is thoroughly washed with distilled water after each use During extended periods of disuse store the sensor with electrode storage solution DO N...

Page 70: ...de dried out Soak the electrode in 3M KCL solution or tap water for 10 minutes Measured value is out of range Check whether the electrode membrane is clogged dirty or broken Incorrect pH buffer soluti...

Page 71: ...Power Off Manual or Automatic 8 minutes after last key pressed Sensor Type Standard pH Electrode Order Code HL101ELEC Power Requirements 2 1 5V AAA Batteries Dimensions 185 L 40 Dia mm Weight 100g...

Page 72: ...rkshire RG19 4HZ Tel 44 0 1635 566772 Fax 44 0 1635 873509 sales Trace2o com www Trace2o com The information in this document is subject to change without notice Copyright Trace2o Limited 2016 All rig...

Page 73: ...Section 8 HydroLite HL102 Pocket EC Tester Instruction Manual...

Page 74: ...mbols to describe the function of each key Map of keys and functions KEY FUNCTION On Off Hold Powers the unit ON OFF Freezes the currently displayed value for recording press the key again to resume m...

Page 75: ...frozen ATC Indicates temperature compensation is enabled Prior to Use Remove the electrode protective cap from unit Soak the electrode for a few minutes in tap water to remove dirt and oil stains on...

Page 76: ...Automatically freezes a stable reading Disable Auto Off Automatically turn off the unit Disable Reset Restore factory settings Disable SETTING THE DEFAULT PARAMETERS 1 Press and hold the CAL key for 3...

Page 77: ...If the auto hold function is enabled the unit will automatically sense a stable endpoint reading and freeze the reading in the display If you disable this function the unit allows user to freeze the...

Page 78: ...freshwater samples 1413 S cm should be sufficient The unit will automatically detect these conductivity standard solutions and prompt the user to calibrate the meter When the calibration is done all n...

Page 79: ...o start the calibration Wait for the reading to stabilise the display shows END The unit returns to measurement mode automatically Single point calibration is completed 2 POINT CALIBRATION 2 1 Ensure...

Page 80: ...it prompts to continue with third point calibration 3 2 Repeat the above steps until the display shows END the unit returns to measurement mode Calibration is completed Note Performing the conductivit...

Page 81: ...onductivity measurement mode Measurement Rinse the conductivity electrode with distilled water Immerse the electrode into the sample solution Stir the solution gently Wait for the reading to stabilise...

Page 82: ...s for calibration standards you need to replace the electrode module 1 Twist the electrode collar counter clockwise pull the old electrode module away from the unit 2 Align the slot on the new electro...

Page 83: ...the electrode module Specifications Conductivity Model HL102 Range 0 20 00 200 0 2000 S cm 20 00mS cm Accuracy 1 F S Resolution 0 01 0 1 1 Calibration Points 1 to 3 points Calibration Solutions 10 S c...

Page 84: ...or To determine the TDS conversion factor use the following formula Factor Where Actual TDS value from the high purity water and precisely weighed NaCl or KCL reagent Actual Conductivity the measured...

Page 85: ...rkshire RG19 4HZ Tel 44 0 1635 566772 Fax 44 0 1635 873509 sales Trace2o com www Trace2o com The information in this document is subject to change without notice Copyright Trace2o Limited 2016 All rig...

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