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Thermo Scientific Sorvall Legend Micro 21R, Instruction Manual

The Thermo Scientific Sorvall Legend Micro 21R is a high-speed refrigerated microcentrifuge designed for rapid sample processing. Ensure proper usage by downloading the Instruction Manual for free from our website. Easily access detailed information on operating procedures and maintenance guidelines to optimize performance.

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Chemical Compatibility    

Chemical Compatibility

MATERIAL

CHEMIC

AL

Aluminium

Anodic Coating for Aluminum

Buna N

Cellulose Acetate Butyrate

Polyurethane Rotor Paint

Composite Carbon Fiber/Epoxy

Delrin™

EPDM rubber

Glass

Neoprene

Noryl™

Nylon

PET¹, Polyclear™,Clear Crimp™

Polyallomer

Polycarbonate

Polyester, Glass Thermoset

Polythermide

Polyethylene

Polypropylene

Polysulfone

Polyvynil Chloride

Rulon A™, Teflon™

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Summary of Contents for Sorvall Legend Micro 21R

Page 1: ...Thermo Scientific Sorvall Legend Micro Series Instruction Manual 50165185 a 07 2021...

Page 2: ...Thermo Scientific Sorvall Legend Micro 21 15 Thermo Scientific Sorvall Legend Micro 17R 16 Thermo Scientific Sorvall Legend Micro 21R 17 1 3 1 Directives and Standards 18 1 4 Mains Supply 20 1 5 Refri...

Page 3: ...CF Value Explained 52 3 5 Entering Centrifugation Parameters 53 3 5 1 Selecting Speed or RCF Value 53 3 5 2 Pre selecting Speed 53 3 5 3 Pre selecting the RCF Value 54 3 5 4 Pre selecting Run Time 54...

Page 4: ...ection 64 4 5 Decontamination 64 4 6 Autoclaving 65 4 7 Service 65 4 8 Lifetime 65 4 9 Shipping 66 4 10 Storage 66 4 11 Disposal 66 5 Troubleshooting 67 5 1 Mechanical Emergency Lid Release 67 5 2 Ice...

Page 5: ...on of Relative Centrifugal Force It separates specimens either alone or after addition of reagents or other additives Together with the hematocrit rotor 75003473 the centrifuge is intended to be used...

Page 6: ...n minor or moderate injury NOTICE Indicates information considered important but not hazard related Symbols used on Centrifuge and Accessories Observe the information contained in this instruction man...

Page 7: ...y instructions The centrifuge is to be used for its intended use only Improper use can cause damages contamination and injuries with fatal consequences The centrifuge must be operated by trained perso...

Page 8: ...and any local regulations When centrifuging microbiological samples from the Risk Group II according to the Laboratory Biosafety Manual of the World Health Organization WHO aerosol tight biological s...

Page 9: ...e occurs Do not open the centrifuge before the rotor has stopped spinning Do not touch a spinning rotor Open the centrifuge only if the rotor has stopped spinning Never use your hands or tools to stop...

Page 10: ...hile it is running The centrifuge housing is not to be opened by the operator CAUTION Due to air friction sample integrity may be affected The temperature of the rotor may rise significantly while the...

Page 11: ...main switch Pull out the power supply plug In an emergency disconnect the power supply Make sure that the main switch and power supply plug can be accessed easily when setting up the centrifuge The g...

Page 12: ...orvall Legend Micro 21R 230 V 10 50 60 Hz 75002446 Sorvall Legend Micro 21R 120 V 10 60 Hz 75002447 Sorvall Legend Micro 21R 100 V 10 50 60 Hz 75002464 Sorvall Legend Micro 21 120 V 10 60 Hz 75002468...

Page 13: ...ocontainment lid 75003418 Dual Row 18 x 2 0 0 5 mL rotor with screw on lid 75003436 36 x 0 5 mL rotor with screw on lid 75003465 10 x 5 mL rotor with ClickSeal biocontainment lid 75003489 PCR 8 x 8 ro...

Page 14: ...verage Heat Dissipation 0 15 kW h Environmental Conditions For Storage and Shipping Temperature 2 C to 50 C For Operation Use in interior spaces Altitudes of up to 3000 m above sea level Temperature 2...

Page 15: ...at Dissipation 0 2 kW h Environmental Conditions For Storage and Shipping Temperature 2 C to 50 C For Operation Use in interior spaces Altitudes of up to 3000 m above sea level Temperature 2 C to 40 C...

Page 16: ...Dissipation 0 25 kW h Environmental Conditions For Storage and Shipping Temperature 2 C to 50 C For Operation Use in interior spaces Altitudes of up to 3000 m above sea level Temperature 2 C to 40 C...

Page 17: ...t Dissipation 0 3 kW h Environmental Conditions For Storage and Shipping Temperature 2 C to 50 C For Operation Use in interior spaces Altitudes of up to 3000 m above sea level Temperature 2 C to 40 C...

Page 18: ...lectronic equipment EN 61010 1 3 1 Edition EN 61010 2 020 3rd Edition EN 61010 2 011 2nd Edition EN 61010 2 101 3rd Edition EN 61326 1 Class B EN ISO 14971 ISO 13485 North America FDA listed Product c...

Page 19: ...nterference to radio communications However there is no guarantee that interference will not occur in a particular installation If this equipment does cause harmful interference to radio or television...

Page 20: ...icro 17 120 60 2 6 180 6 3 AT 15A 75002432 Sorvall Legend Micro 17 100 50 60 2 9 170 6 3 AT 15A 75002433 Sorvall Legend Micro 17 230 50 60 1 4 180 4AT 16A 75002435 Sorvall Legend Micro 21 230 50 60 1...

Page 21: ...A 2 pole circuit breaker 15A 75002464 Sorvall Legend Micro 21 120 60 3 4 220 6 3 AT 15A 75002468 Sorvall Legend Micro 21 100 50 60 3 9 230 6 3 AT 15A 75002469 Sorvall Legend Micro 21 230 50 60 1 7 230...

Page 22: ...120 60 3 9 330 8A 2 pole circuit breaker 15A 75002544 Sorvall Legend Micro 21R 230 50 60 2 2 370 4A 2 pole circuit breaker 16A 75002545 Sorvall Legend Micro 21R 100 50 60 5 1 360 8A 2 pole circuit br...

Page 23: ...34a 0 26 kg 21 bar 1430 0 37 t 75002432 Sorvall Legend Micro 17 R 134a 0 26 kg 21 bar 1430 0 37 t 75002433 Sorvall Legend Micro 17 R 134a 0 26 kg 21 bar 1430 0 37 t 75002435 Sorvall Legend Micro 21 R...

Page 24: ...6 kg 21 bar 1430 0 37 t 75002464 Sorvall Legend Micro 21 R 134a 0 26 kg 21 bar 1430 0 37 t 75002468 Sorvall Legend Micro 21 R 134a 0 26 kg 21 bar 1430 0 37 t 75002469 Sorvall Legend Micro 21 R 134a 0...

Page 25: ...Micro 17R R 134a 0 26 kg 21 bar 1430 0 37 t 75002544 Sorvall Legend Micro 21R R 134a 0 26 kg 21 bar 1430 0 37 t 75002545 Sorvall Legend Micro 21R R 134a 0 26 kg 21 bar 1430 0 37 t 75002557 Sorvall Leg...

Page 26: ...rature 121 C Table 11 General Technical Data 24 x 1 5 2 0 mL Rotor Rotor Performance Data 17 Series Centrifuges 24 x 1 5 2 0 mL Rotor Voltage 230 V 120 V Maximum Speed 13300 13300 Maximum RCF Value 17...

Page 27: ...Acceleration Braking Time 12 s 13 s 12 s 13 s Min Temperature at max Speed Ambient Temperature of 23 C 0 C 0 C Table 12 24 x 1 5 2 0 mL Rotor Performance Data Accessories Description Article No Rotor...

Page 28: ...15 General Technical Data Dual Row 18 x 2 0 0 5 mL Rotor Rotor Performance Data 17 Series Centrifuges Dual Row 18 x 2 0 0 5 mL Rotor Voltage 230 V 120 V Maximum Speed 13300 13300 Maximum RCF Value 16...

Page 29: ...f 23 C 0 C 0 C 21R Series Centrifuges Dual Row 18 x 2 0 0 5 mL Rotor Voltage 230 V 120 V Maximum Speed 14800 14800 Maximum RCF Value 20800 20800 Acceleration Braking Time 11 s 13 s 11 s 13 s Min Tempe...

Page 30: ...General Technical Data 36 x 0 5 mL Rotor Rotor Performance Data 17 Series Centrifuges 36 x 0 5 mL Rotor Voltage 230 V 120 V Maximum Speed 13300 13300 Maximum RCF Value 15600 15600 Acceleration Brakin...

Page 31: ...Temperature of 23 C 0 C 0 C 21R Series Centrifuges 36 x 0 5 mL Rotor Voltage 230 V 120 V Maximum Speed 14800 14800 Maximum RCF Value 19300 19300 Acceleration Braking Time 9 s 11 s 9 s 11 s Min Tempera...

Page 32: ...erature 121 C Table 23 General Technical Data 10 x 5 mL Rotor Rotor Performance Data 17 Series Centrifuges 10 x 5 mL Rotor Voltage 230 V 120 V Maximum Speed 13300 13300 Maximum RCF Value 16414 16414 A...

Page 33: ...ies Centrifuges 10 x 5 mL Rotor Voltage 230 V 120 V Maximum Speed 14800 14800 Maximum RCF Value 20326 20326 Acceleration Braking Time 12 s 13 s 12 s 13 s Min Temperature at max Speed Ambient Temperatu...

Page 34: ...able 27 General Technical Data PCR 8 x 8 Rotor Rotor Performance Data 17 Series Centrifuges PCR 8 x 8 Rotor Voltage 230 V 120 V Maximum Speed 13300 13300 Maximum RCF Value 13800 13800 Acceleration Bra...

Page 35: ...t Temperature of 23 C 0 C 0 C 21R Series Centrifuges PCR 8 x 8 Rotor Voltage 230 V 120 V Maximum Speed 14800 14800 Maximum RCF Value 17100 17100 Acceleration Braking Time 7 s 9 s 7 s 9 s Min Temperatu...

Page 36: ...e 31 General Technical Data PCR 4 x 8 Rotor Rotor Performance Data 17 Series Centrifuges PCR 4 x 8 Rotor Voltage 230 V 120 V Maximum Speed 13300 13300 Maximum RCF Value 13100 13100 Acceleration Brakin...

Page 37: ...aximum Speed 14800 14800 Maximum RCF Value 16200 16200 Acceleration Braking Time 11 s 13 s 11 s 13 s Min Temperature at max Speed Ambient Temperature of 23 C 0 C 0 C Table 32 PCR 4 x 8 Rotor Performan...

Page 38: ...und report it to the carrier and request a damage inspection Important Failure to request an inspection of damage within a few days after receipt of shipment absolves the carrier from any liability fo...

Page 39: ...o compensate for an uneven surface Do not operate the centrifuge on carts or stand alone shelving that could move during operation or is inadequately sized for the centrifuge Be able to hold the weigh...

Page 40: ...40 94 Transport and Set Up Figure 1 Safety Zone...

Page 41: ...t the centrifuge the centrifuge door is closed CAUTION Hands can be squeezed by an open centrifuge door Always close the centrifuge door before you transport the centrifuge Before transporting a rotor...

Page 42: ...2 Make sure that the power supply cable specification agrees with the safety standards of your country 3 Make sure that the voltage and frequency are the same as the figures on the nameplate 4 Make su...

Page 43: ...he centrifugation chamber and the empty rotor before the centrifugation run starts using SNOWFLAKE button NOTICE This function is only available on refrigerated centrifuges PULSE Press PULSE to immedi...

Page 44: ...a special switch for balancing potential voltage discrepancies in the power grid After pressing the mains switch the display therefore may still flash up to 10 seconds 3 2 3 Audible Alarm Error Accomp...

Page 45: ...it lightly in the middle or on both sides The locking mechanism engages to close the lid safely The lid should audibly click into place Double check whether the locking mechanism has engaged properly...

Page 46: ...fuge spindle The two bars in the labeling on the upper side of the rotor a must be aligned with the retaining pin b of the centrifuge spindle a b 3 Let the rotor slide down slowly 4 Insert the Allen w...

Page 47: ...be hot 55 C Be aware of this risk and proceed carefully when you change a rotor after a run or wait until the motor has cooled down Before installing a rotor Remove any dust foreign objects or residue...

Page 48: ...1 Put the rotor lid on the rotor nut 2 Push the rotor lid down until you see and hear the lock click If the lid does not close at all or only by force make sure that the sealing rings are correctly i...

Page 49: ...otor Lid Seals CAUTION Loose parts may damage the centrifuge When operating the rotor without the lid the seals are not fixed in their position and can damage the centrifuge Tube Caps Always close the...

Page 50: ...rrosion 2 Inspect the centrifugation chamber and drive shaft for damage such as cracks scratches or traces of corrosion 3 Check the suitability of the rotor and other used accessories against the Chem...

Page 51: ...um Loading Each rotor is designed to run with its maximum load at maximum speed The safety system of the centrifuge requires that the rotor is not overloaded The rotors are designed to work with subst...

Page 52: ...maximum RCF value is related to the maximum radius of the tube opening Remember that this value is reduced depending on the tubes buckets and adapters used This can be accounted for in the calculatio...

Page 53: ...eed 1 Enter the desired value by pressing up and down ARROW buttons repeatedly until the desired value shows You can adjust the speed in 100 rpm increments 2 Press START to confirm the pre selected va...

Page 54: ...f centrifuge radius of the 24 x 1 5 2 0 mL Rotor 75003424 Refer to RCF Value Explained on page 52 for further information NOTICE Avoid if possible speed ranges close to natural resonances of the syste...

Page 55: ...determine the sample temperature in C Proceed as follows 1 Enter the desired value by pressing the up or down ARROW buttons under the right display repeatedly until the desired value shows You increa...

Page 56: ...2 Enter the desired value by pressing the up or down ARROW buttons under the right display repeatedly until the desired value shows You increase or decrease the temperature in steps of 1 C 3 Press STA...

Page 57: ...t be kept out of this safety zone while centrifuging Once the main switch has been turned on the rotor has been properly installed the setpoints have been set as explained in the previous section and...

Page 58: ...l you release the key The centrifuge accelerates to and brakes at maximum power The pre selected value is ignored NOTICE The centrifuge accelerates to maximum speed The run time is initially displayed...

Page 59: ...p detail Large seal in outer groove of rotor body bottom detail small seal in groove of rotor collar 1 Lubricate all seals 2 Press the large seal in the outer groove of the rotor body top detail 3 Pre...

Page 60: ...id Quick Test As a quick test it is possible to test the aerosol tightness of fixed angle rotors using the following process 1 Lubricate all seals lightly Always use the supplied grease when lubricati...

Page 61: ...thoroughly cleaning the rotors they must be inspected for damage wear and corrosion Make sure that sealing rings are still smooth not brittle nor otherwise damaged Some sealing rings are not autoclav...

Page 62: ...re of 50 C If drying boxes are used the temperature must never exceed 50 C Higher temperatures could damage the material and shorten the lifetime of the parts 8 Inspect the rotor and accessories for s...

Page 63: ...ifuge to the edge of the table 2 Pull the clip below the sucking grid and remove the filter unit completely by pulling it down 3 Remove the cumulated dust with a soft cloth 4 Inserting the filter agai...

Page 64: ...agent Observe the safety precautions and handling instructions for the disinfection agents used 4 5 Decontamination You are responsible that the level of decontamination is achieved according to your...

Page 65: ...g electrical equipment and connections suitability of set up site centrifuge lid lock and safety system rotor fixation of rotor and drive shaft of the centrifuge protective casing Before service centr...

Page 66: ...move the centrifuge and accessories from use clean and if necessary disinfect or decontaminate the full system If you are not sure speak to the Thermo Fisher Scientific customer service 4 11 Disposal...

Page 67: ...out braking The brake does not work when there is no power The braking process lasts much longer than usual Proceed as follows 1 Wait until the rotor has stopped This can take several minutes Use the...

Page 68: ...47 3 Let the ice melt NOTICE Do not use any sharp tools aggressive liquids or fire to fasten the melting process If necessary use warm water to speed up the melting process 4 Remove the water from the...

Page 69: ...ation to standstill Centrifuge lid was opened manually during the run Close centrifuge lid immediately Rotor stops with deceleration to standstill For further centrifugation you have to switch the ins...

Page 70: ...respectively the filter unit of the cooled centrifuge After approx 60 minutes you can restart the instrument Observe the maximum permissible environmental temperature If the error message still shows...

Page 71: ...technician Table 34 Troubleshooting 5 3 8 Information for the Customer Service If you need to contact customer service please provide the order no and the serial no of your device This information can...

Page 72: ...ptoethanol S S U S M S S U S S U S S S S S S U S S S S S S A cetaldehyde S U U M U M U U U M M M S U S U A cetone M S U U S U M S S U U S U S U U U S S U U S M M S U U A cetonitrile S S U S M S S S U...

Page 73: ...U U S M U U S S U S U S U A mmonium A cetate S S U S S S S S S S S S S U S S S S S S S S S S A mmonium C arbonate M S U S S S S S S S S S S S U U S S S S S S M S S S A mmonium H ydroxide 10 U U S U S...

Page 74: ...S U S S S S S S S S S S S S S S S U S S U A myl A lcohol S M U S S M S M S S S S M U S M A niline S S U U S U S M S U U U U U U U S M U U S S S S U S S odium H ydroxide 1 U M S S S S M S S S M M S S S...

Page 75: ...S S S S S S S M S S S C esium B romide M S S S S S S S S S S S S S S S S S S M S S S C esium C hloride M S S U S S S S S S S S S S S S S S S S M S S S C esium F ormate M S S S S S S S S S S S S S S S...

Page 76: ...ol M ixture S S U S S U U U U U U U U U S S S S U S C yclohexane S S S S S S U S U S S U U U M S M U M M S U M M U S D eoxycholate S S S S S S S S S S S S S S S S S S S S S S S D istilled W ater S S S...

Page 77: ...ulfoxide S S U U S S S S U S S U S U U S S U U S S S S U U D ioxane M S U U S S M M S U U S U M U U M M M U S S S S U U F erric C hloride U U S M S M S S S S M U S S A cetic A cid G lacial S S U U S S...

Page 78: ...50 S S S S S S M S S S S S U S U S S S S S S S S M S M U E thyl A lcohol 95 S S S U S S M S S S S S U S U S S S M S S S U S M U E thylene D ichloride S U U S M U U S U U U U U U U U S U S S E thylene...

Page 79: ...ofluoric A cid 10 U U U M U U U S S M U S S S S M S U U U H ydrofluoric A cid 50 U U U U U U U U U S U U U S S M M S U U U M H ydrochloric A cid conc U U U U U U M U M U U M U U U S U S U U U F ormald...

Page 80: ...mo S ol S S S S S S S S S S S S S S S S S S S S S H exane S S S S S S S S U S U M U S S U S S M S U S S U S I sobutyl A lcohol M U S S U S U S S M S S S S S S S S I sopropyl A lcohol M M M U S S S S S...

Page 81: ...S S S S S S S S S S S S S S S S S S S U S S S P otassium H ydroxide 5 U U S S S S M S S S S S U S S S S S S S M U M S U P otassium H ydroxide conc U U M U M M S S U M U U U S M M U U U U P otassium P...

Page 82: ...n Viton K erosene S S S S S S U S M U S U M M S M M M S S U S S U S S odium C hloride 10 S S S S S S S S S S S S S S S S S S M S S odium C hloride sat d U S U S S S S S S S S S S S S S M S C arbon T e...

Page 83: ...S S S S S S M S S S M ercaptoacetic A cid U S U S M S S M S U U U U S U U S M S U S S S S M ethyl A lcohol S S S U S S M S S S S S U S U M S S S S S S S M S M U M ethylene C hloride U U U U M S S U S...

Page 84: ...M S S S N B utyl A lcohol S S U S S M U S M S S S S M M S M S S N B utyl P hthalate S S U S S S S U U S U U U M U U S U S M M S U S N N D imethylformamide S S S U S M S S S U S U S U U S S U U S M S...

Page 85: ...U S S M S S S M S S S S M S S S M U S M S S odium I odide M S S S S S S S S S S S S S S S S S S M S S S S odium N itrate S S S S S S S S S S S S S S S S S S S S U S S S S S odium S ulfate U S S S S S...

Page 86: ...S S U S S S S S S M S O leic A cid S U S S S U U S U S S M S S S S S S S S S M U S M M O xalic A cid U U M S S S U S S S S S U S U S S S S S S S S U M S S P erchloric A cid 10 U U S U U S M M M U M S...

Page 87: ...S S M S M S U M U S P hysiologic M edia S erum U rine M S S S S S S S S S S S S S S S S S S S S S S S P icric A cid S S U S M S S S M S U S S S U S S S S U S U M S M S P yridine 50 U S U U S U U U S S...

Page 88: ...S U U S U S U S S S S S S S S S M S S S S N itric A cid 50 U S U M S U U S U S U U M M U M M M S S S U S S M S N itric A cid 95 U U U U U U U U U M U U U U M U U S U S S S H ydrochloric A cid 10 U U M...

Page 89: ...U U S U U U M S U M S U U U S S tearic A cid S S S M S S S S S S S S S S S S S M M S S S T etrahydrofuran S S U U S U U M S U U S U U U M U U U U S U S S U U T oluene S S U U S S M U S U U S U U U S...

Page 90: ...S S S T ris B uffer neutral p H U S S S S S S S S S S S S S S S S S S S S S S S S S T riton X 100 S S S S S S S S S S S S S S S S S S S S S S S S S U rea S U S S S S S S S M S S S S S S S M S S H ydro...

Page 91: ...S S S S S S S U S S S Z inc S ulfate U S S S S S S S S S S S S S S S S S S S S S S S S S C itric A cid 10 M S S M S S M S S S S S S S S S M S S S S S S S S S S S Satisfactory M Moderate attack may be...

Page 92: ...atocrit Rotor 33 How to install a rotor 42 How to Operate a Rotor 42 How to remove a rotor 43 I Ice Formation 64 Information for the Customer Service 67 Intended Use 5 Items Supplied 34 L List of Cent...

Page 93: ...Standards 17 Storage 39 T Technical Data 13 Technical Specifications 12 Transport and Set Up 34 Troubleshooting 63 Troubleshooting by Guide 64 U Unpacking 34...

Page 94: ...oll free 0800 1 536 376 Germany international 49 6184 90 6000 India 91 22 6716 2200 Italy 39 02 95059 552 Japan 81 3 5826 1616 Netherlands 31 76 579 55 55 New Zealand 64 9 980 6700 Nordic Baltic CIS c...

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