Thermo Scientific PikoReal, User Manual

Page: 34 / 72

Routine Operation
Monitoring a run
32 Thermo Scientific PikoReal Real-Time PCR System User Manual Thermo Fisher Scientific
Note If less than 16 x 0.2 ml tubes have been loaded and > 16 TUBES
OR PLATE is selected by mistake, the lid pressure may cause the tubes
to become crushed or deformed. ▲
Note
If you are using single tubes, you must use at least eight tubes that
are placed symmetrically with at least one tube in each corner of the
block. You can use empty tubes to fill the 8-tube requirement when
analyzing fewer samples.
▲
8. Enter the sample volume (µl per well) using the arrow keys.
●
24-well PikoReal: 10–50 µl
●
96-well PikoReal: 5–20 µl
9. Press Enter to confirm the desired volume.
Note When a run begins, the lid inside the instrument will begin
moving, a blue indicator light will flash, and the running protocol will
be displayed in semi-graphical mode on a Status screen (see
“Monitoring a run” on page 32). ▲
You can monitor a run in two screens: Status screen and Time status
screen.
Upon initiation of a run, initialization tests are carried out and the
Status screen is displayed. The current temperature step in the protocol
is represented by a flashing line (graph view) or highlighted (list view),
and the protocol and host folder are displayed on the top of the screen.
Monitoring a run
Status screen