Thermo Scientific PikoReal, User Manual

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Introduction to the PikoReal( Real-Time PCR System
Safety instructions
12 Thermo Scientific PikoReal Real-Time PCR System User Manual Thermo Fisher Scientific
reagent dispensers and automated liquid handling systems. The
PikoReal 24 also utilizes standard low profile 8-well strips and 0.2 ml
tubes.
The optical system comprises a charge-coupled device (CCD) camera,
optical filters and an individual light-emitting diode (LED) for each of
the five channels. The channel selection is built on a motorized linear
shuttle that moves to five channel positions.
The PikoReal is controlled by PikoReal Software via an Ethernet
connection (with up to 10 connections) or alternatively as a standalone
instrument with USB memory stick loaded protocols.
The PikoReal Real-Time PCR System has the following features:
●
Compact design – The PikoReal Real-Time PCR System has a
compact design giving a minimal footprint for personal benchtop
use and field applications.
●
Unique thermal system – Three Peltier elements and a patented
heat sink design provide a fast and even heat distribution and
removal.
●
5 LED optical system – Separate LEDs for each channel offer a
wide excitation range (475–640 nm) and multiplexing for four
dyes.
●
Piko
®
format – The PikoReal Real-Time PCR System sample
blocks (24-well and 96-well) will only accept microplates with a
footprint with the dimensions of a microscope slide – the Piko
format. The 24-well sample block is also compatible with all
standard low-profile 8-tube strips and single tubes.
●
Automatic lid – The heated lid is internally motor-controlled and
the lid will automatically adjust to the optimal temperature and
sealing pressure.
●
External power supply – The PikoReal Real-Time PCR System
uses an external 200 watt power supply (an approach commonly
used with lap-top computers).
Note Conventional format full-sized microplates are not compatible
with the PikoReal blocks. ▲
The PikoReal Real-Time PCR System is available in two different
configurations:
●
PikoReal 24
•
Cat. no. TCR0024
•
for use with 24-well Piko PCR plates, standard 8-well strips
and 0.2 ml tubes
●
PikoReal 96
•
Cat. no. TCR0096