Thermo Scientific PikoReal, User Manual

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Introduction to the PikoReal( Real-Time PCR System
Safety instructions
Thermo Fisher Scientific Thermo Scientific PikoReal Real-Time PCR System User Manual 11
Chapter 1
Introduction to the PikoReal
®
Real-Time PCR System
The PikoReal Real-Time PCR System (Figure 1-1) is a gene
quantification and genotyping platform in 24- and 96-well block
formats. Using PikoReal Software, the system is applicable to absolute
quantification, relative quantification, melt curve analysis and allelic
discrimination. The PikoReal Real-Time PCR System is designed to a
minimal footprint, making the system ideal for personal benchtop use
and field applications, for example.
Figure 1-1. PikoReal 96 Real-Time PCR System
The combination of several innovative technologies applied in design,
including optics, unique heat sink design, block design, plate size and
the usage of special plasticware, create many advantages over
conventional qPCR systems. The PikoReal Real-Time PCR System is
fast, fulfills high-level requirements of temperature uniformity over the
wells and creates significant savings in reagent, plastic material and
energy consumption.
The PikoReal Real-Time PCR System uses ultra-thin wall (UTW
®
)
microwell plates that are 1/4 of the size of conventional microwell
plates. The plates adhere to industry standards regarding well spacing
and sample capacity and are compatible with multichannel pipettes,