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Thermo Scientific PikoReal

Real-Time PCR System

User Manual

Rev. 2.0

Summary of Contents for PikoReal

Page 1: ...Thermo Scientific PikoReal Real Time PCR System User Manual Rev 2 0...

Page 2: ......

Page 3: ...Thermo Scientific PikoReal Real Time PCR System User Manual Rev 2 0 Cat no N11471...

Page 4: ...y California 94404 USA Trademarks ALPS DyNAmo Finnpipette Matrix Maxima Multidrop Piko PikoReal Solaris Versette Verso and UTW are trademarks or registered trademarks of Thermo Fisher Scientific Inc a...

Page 5: ...bleshooting the instrument This user manual also describes all the features and specifications of the PikoReal Real Time PCR System and provides ordering information for instruments spare parts reagen...

Page 6: ...off Serial number Catalog number Date of manufacture Consult instructions for use WEEE symbol This product is required to comply with the European Union s Waste Electrical Electronic Equipment WEEE D...

Page 7: ...able of handling electrical equipment should use the PikoReal Real Time PCR System NOT FOR CHILDREN Do Not Attempt to Repair the System Do not remove the cover of the system Do not try to repair or re...

Page 8: ...About This User Manual Safety instructions 6 Thermo Scientific PikoReal Real Time PCR System User Manual Thermo Fisher Scientific...

Page 9: ...13 General Description 15 Instrument views 15 PikoReal user interface 17 Status indicator 17 Power supply 17 PikoReal Software 18 Block formats 18 Consumables 18 UTW technology 19 Reagents 20 Dye cali...

Page 10: ...a to USB 39 Shutting down 40 Maintenance 41 Regular and preventive maintenance 41 Maintenance checklist 41 Instrument care 41 Cleaning of the instrument 42 Cleaning the block and wells 43 Cleaning the...

Page 11: ...l Real Time PCR System User Manual 9 Ordering Information 55 PikoReal 55 List of spare parts and accessories 55 List of consumables 56 List of reagents 57 List of heat sealers 57 System Log 59 Certifi...

Page 12: ...Contents 10 Thermo Scientific PikoReal Real Time PCR System User Manual Thermo Fisher Scientific...

Page 13: ...personal benchtop use and field applications for example Figure 1 1 PikoReal 96 Real Time PCR System The combination of several innovative technologies applied in design including optics unique heat s...

Page 14: ...s Unique thermal system Three Peltier elements and a patented heat sink design provide a fast and even heat distribution and removal 5 LED optical system Separate LEDs for each channel offer a wide ex...

Page 15: ...amount of DNA formed Collecting quantitative data of a known dilution series of the target DNA can be used to give insight into the PCR reaction kinetics and reaction efficiency The gene quantificati...

Page 16: ...ific PikoReal Real Time PCR System User Manual Thermo Fisher Scientific Caution Operate the instrument only with software and hardware that is specifically designed or selected for it Thermo Fisher Sc...

Page 17: ...me PCR System User Manual 15 Chapter 2 General Description This chapter describes the main parts of the PikoReal instrument The front view of the PikoReal instrument is shown in Figure 2 2 Figure 2 2...

Page 18: ...hown in Figure 2 3 Figure 2 3 PikoReal rear view Caution Do not block the air intake or the air exhaust vents Figure 2 2 and Figure 2 3 Be careful not to place for example a piece of paper under the a...

Page 19: ...d in use Constant red System error For more information see Chapter 7 Troubleshooting Guide The PikoReal Real Time PCR System is equipped with an external 200 watt power supply A green light on the po...

Page 20: ...eal Real Time PCR instrument is available in two block formats Figure 2 5 and Figure 2 6 Figure 2 5 24 well 50 l max volume for PCR Figure 2 6 96 well 20 l max volume for PCR PikoReal 24 well and 96 w...

Page 21: ...optical caps Not available in US Note If you are using single tubes you must use at least eight tubes that are placed symmetrically with at least one tube in each corner of the block You can use empt...

Page 22: ...ll thickness of UTW consumables marked as blue is half that of standard consumables marked as red Figure 2 9 The lower portion of the UTW reaction vessels is ultra thin For more information on the con...

Page 23: ...efer to Appendix A Color Calibration in the PikoReal Software User Manual Cat no N12076 Thermo Fisher Scientific provides a variety of dispensers compatible with Piko PCR plates Finnpipette and Matrix...

Page 24: ...General Description Heat sealers 22 Thermo Scientific PikoReal Real Time PCR System User Manual Thermo Fisher Scientific...

Page 25: ...ment onto a laboratory bench Caution Do not touch or loosen any screws or parts other than those specifically designated in the instructions Doing so may cause misalignment and will void the instrumen...

Page 26: ...perform optimally and exhibit the highest reliability within the recommended environmental ranges Table 3 2 Although the instrument is rated to operate reliably within the absolute environmental rang...

Page 27: ...other instruments exhaust directly at the PikoReal Real Time PCR System Clean the fins of the heat sink and the instrument regularly see Cleaning the heat sink on page 43 This section describes the in...

Page 28: ...User Manual Cat no N12076 The data port see Figure 2 2 on page 15 is to be used with USB memory devices only For instructions on how to use a USB memory device refer to Running a protocol on page 30...

Page 29: ...pply Before you put the instrument into use perform the following operational check 1 Plug the instrument into the mains The instrument automatically proceeds to perform the self tests The self tests...

Page 30: ...Installation Installing PikoReal Software 28 Thermo Scientific PikoReal Real Time PCR System User Manual Thermo Fisher Scientific...

Page 31: ...ypad are two indicator lights with the following indications Constant blue System is on and NOT in use Blinking blue System is on and in use Constant red System has recently experienced an error or wa...

Page 32: ...al Software refer to the PikoReal User Software Manual Cat No N12076 To run a protocol from USB or from the instrument memory 1 Select RUN in the Main menu and press Enter 2 If a USB is attached the U...

Page 33: ...ing the view mode list or graph start a run by selecting START and pressing Enter With a 24 well PikoReal Real Time PCR System the users will be presented with consumable and volume options With a 96...

Page 34: ...olume l per well using the arrow keys 24 well PikoReal 10 50 l 96 well PikoReal 5 20 l 9 Press Enter to confirm the desired volume Note When a run begins the lid inside the instrument will begin movin...

Page 35: ...of the screen display the time hours minutes and seconds remaining until the completion of the running protocol The file name and host folders are displayed on the top of the screen The following sta...

Page 36: ...ovides six options which are accessed using the arrow keys and then by pressing Enter SYSTEM INFO Displays system info DIAGNOSTICS Allows users to check lid parameters sensor parameters display settin...

Page 37: ...of the PIKO module i e the thermal cycler module RT Displays the firmware version of the RT module i e the real time module Select DIAGNOSTICS in the TOOLS menu with the arrow keys and press Enter Th...

Page 38: ...cted Note that this is not possible during a run Select DIAGNOSTICS in the TOOLS menu with the arrow keys and press Enter Select SENSORS with the arrow keys and press Enter The Sensors screen displays...

Page 39: ...STICS in the TOOLS menu with the arrow keys and press Enter Select CLOCK with the arrow keys and press Enter The Clock screen allows viewing of the PikoReal s internal date and time settings Use the P...

Page 40: ...his function is to be used by authorized Thermo Fisher Scientific service personnel only Select LOGFILES in the TOOLS menu with the arrow keys and press Enter The Logfiles screen allows the user to vi...

Page 41: ...gure 2 3 on page 16 When you restart the instrument a prompt appears to check that you have removed the transport locking screw from the shipping position Note In the new models of the instrument the...

Page 42: ...ry stick once the protocol is ready This option is disabled while the protocol is active Note Thermo Fisher Scientific does not guarantee that all USB memory devices are compatible with the instrument...

Page 43: ...prevent damage and wipe with deionized distilled water See Cleaning of the instrument on page 42 3 If any surfaces have been contaminated with biohazardous material disinfect with a mild sterilizing...

Page 44: ...t your local Thermo Fisher Scientific representative for service Caution Although the PikoReal Real Time PCR System is constructed from high quality materials you must immediately wipe away spilled sa...

Page 45: ...art of this instrument To clean the block and wells Figure 2 2 Figure 2 5 and Figure 2 6 follow the instructions below 1 Turn the power off and unplug the instrument 2 Wear disposable gloves to protec...

Page 46: ...dure should be performed by authorized and trained personnel in a well ventilated room wearing disposable gloves protective glasses and clothing Decontamination should be performed in accordance with...

Page 47: ...cate of Decontamination both inside the transport package and attached to the outside of the package To pack the PikoReal Real Time PCR System for service follow the instructions presented below Infor...

Page 48: ...rument must be carried out to ensure that there is no risk of contamination Warning Decontaminate the instrument before disposal Refer to Decontamination procedure on page 44 Follow laboratory and cou...

Page 49: ...pply and cord Electrical Power supply 200 W 24 V 8 3 A Line voltage 90 240 VAC Input current 2 5 A and 4 0 A Frequency 50 60 Hz Power failure recovery Yes for 24 hours Instrument configuration Block f...

Page 50: ...650 nm Channel 4 Excitation range 600 640 nm Emission range 666 740 nm Channel 5 Excitation range 475 500 nm Emission range 520 590 nm This section describes the safety specifications for the PikoRea...

Page 51: ...4 1 see Note 2 Note 1 The installation category overvoltage category defines the level of transient overvoltage which the instrument is designed to withstand safely It depends on the nature of the ele...

Page 52: ...Technical Specifications Safety specifications 50 Thermo Scientific PikoReal Real Time PCR System User Manual Thermo Fisher Scientific...

Page 53: ...fic representative In case of less than a 24 hour power failure the instrument continues the interrupted run from the same protocol step it was performing at the time of the interruption If the protoc...

Page 54: ...HEATSINK IS CLEAN W27 PROTOCOL STOPPED This warning is set if the protocol is stopped before the end of the protocol W28 CAMERA FAILURE This warning is set if the first camera plate read command fail...

Page 55: ...ck temperature changes more than 10 C during a 500 ms interval REBOOT INSTRUMENT IF PROBLEM PERSISTS CONTACT SUPPORT E17 LID TEMP SHIFT This error is set if the lid temperature changes more than 20 C...

Page 56: ...t REBOOT INSTRUMENT IF PROBLEM PERSISTS CONTACT SUPPORT E35 LID TEMP CHANGE This error is set if the lid is active the temperature difference to the target is more than 20 C and the response to a 10 s...

Page 57: ...le 8 8 Codes for spare parts and accessories Code Item Qty N11471 PikoReal Real Time PCR System User Manual 1 N12076 PikoReal Software User Manual 1 N12119 PikoReal Software for PikoReal Real Time PCR...

Page 58: ...cientific flat 8 cap strips for Piko PCR Plates optically clear 120 strips AB 0812 Clear Seal Diamond Heat Sealing Film 100 seals TUC0010 Thermo Scientific Piko UTW individual tubes clear tube clear c...

Page 59: ...Catalog number is product specific Please refer to www thermoscientific com solaris to select the appropriate assay Each pre designed Solaris qPCR Assay consists of a single probe 200 nM and primer pa...

Page 60: ...Ordering Information List of heat sealers 58 Thermo Scientific PikoReal Real Time PCR System User Manual Thermo Fisher Scientific...

Page 61: ...System Log Thermo Fisher Scientific Thermo Scientific PikoReal Real Time PCR System User Manual 59 Appendix A System Log Instrument name number User Date Comments PHOTOCOPIABLE...

Page 62: ...System Log 60 Thermo Scientific PikoReal Real Time PCR System User Manual Thermo Fisher Scientific...

Page 63: ...turned items have been decontaminated and can be handled without exposing the personnel to health hazards Materials used in the unit Chemicals Biological Radioactive Specific information about contami...

Page 64: ...Certificate of Decontamination 62 Thermo Scientific PikoReal Real Time PCR System User Manual Thermo Fisher Scientific...

Page 65: ...rcalator is a type of molecule that binds to DNA and inserts itself into the DNA structure LED Light emitting diode MAC Short for Media Access Control address a hardware address that uniquely identifi...

Page 66: ...Glossary 64 Thermo Scientific PikoReal Real Time PCR System User Manual Thermo Fisher Scientific...

Page 67: ...isposal of instrument 46 disposal of materials 44 DNA analysis 13 drawer 16 36 44 45 54 dye calibration 21 E emission 48 environmental requirements 24 25 error 17 27 29 46 51 52 53 54 codes 27 51 52 m...

Page 68: ...48 55 56 63 cycle 13 method 13 product 13 PIKO 35 plate 11 18 19 31 32 52 53 54 55 56 drawer 16 36 44 45 54 power supply 2 12 17 18 24 25 26 44 47 51 55 protocol 27 29 30 31 32 33 34 38 40 51 52 54 Q...

Page 69: ...o Scientific PikoReal Real Time PCR System User Manual 67 U uniformity 11 47 USB 12 26 29 30 31 34 39 40 47 user interface 17 29 63 UTW 2 11 13 19 20 32 40 44 45 56 63 W warning codes 26 51 52 warrant...

Page 70: ...68 Thermo Scientific PikoReal Real Time PCR System User Manual Thermo Fisher Scientific Notes...

Page 71: ......

Page 72: ...Thermo Fisher Scientific Oy Ratastie 2 P O Box 100 FI 01621 Vantaa Finland www thermoscientific com N11471...

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