1X Tris-Borate EOTA Buffer (TAE)
1 X TBE is used for agarose and polyacrylamide gel electrophoresis and
semidry electroblotting of nucleic acids:
Final 1 X composition:
0.04M Tris Acetate
0.001 M disodium EDTA
pH 8.0
1X Tris-Glycine-SOS Buffer (TGS)
1 X TGS buffer is used for denaturing polyacrylamide gel electrophoresis
of proteins.
Final 1 X composition:
0.025M Tris Base
0.192M Glycine
0.1% SDS
pH 8.3
1X Three Buffer System for Semidry Electroblotting
This buffer is used with the HEP-1 Semidry Electroblotter
Final 1 X composition:
Anode 1 Buffer: 0.3M Tris Base, 20% MeOH, pH 10.4
Anode 2 Buffer: 0.025M Tris Base, 20% MeOH pH 10.4
Cathode Buffer: 0.025M Tris Base, 0.04M Caproic Acid, 20% MeOH
pH 9.4
NAQ Northern Transfer Buffer (8)
For transfer of RNA from agarose gels. With its high buffering capacity
and low ionic strength, this buffer is more efficient than TAE, TBE or
MOPS from agarose gels.
50X:
0.2M morpholinopropanesulfonic acid (MOPS)
50mM sodium acetate
5mM EDTA
pH 7.0
5-4
Semidry Electroblotter
Thermo Scientific
Section 5
Technical Tips
Recipes for Buffers
(continued)
