Thermo Scientific
ROSS Ultra, ROSS Ultra Triode and ROSS pH Electrodes
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Thermo Scientific Orion
1. a. For general cleaning, prepare a 0.1 M hydrochloric acid (HCl)
or 0.1 M nitric acid (HNO
3
) solution.
b. For cleaning oil or grease, prepare a 0.1 to 0.5 % liquid
detergent solution mixed with hot water.
c. For cleaning bacterial contaminants, prepare a 1:10 dilution of
household laundry bleach.
2. a. Soak the electrode in the 0.1 M HCl or HNO
3
for half an hour
and stir the solution at a moderate rate.
b. Soak the electrode in the 1:10 dilution of household laundry
bleach or the 0.1 to 0.5% liquid detergent solution mixed with
hot water for 15 minutes and stir the solution at a moderate
rate.
3. If a refillable electrode is used, drain the reference chamber and
refill the electrode with fresh ROSS fill solution.
4. Soak the electrode in ROSS pH electrode storage solution, Cat.
No. 810001, or standard pH electrode storage solution, Cat. No.
910001, for at least one hour.
Cleaning Solutions
Cat. No. 900021-WA: pH cleaning solution A for removing protein
contaminants.
Cat. No. 900022-WA: pH cleaning solution B for removing bacterial
contaminants.
Cat. No. 900023: pH cleaning solution C for general cleaning.
Cat. No. 900024: pH cleaning solution D for removing oil and grease
contaminants.
Cat. No. 900020: pH cleaning solution kit, includes cleaning solutions
A, B, C and D.
Electrode Characteristics
Temperature Effects
The most common cause of error in pH measurements is temperature.
There are at least five ways that temperature variations can affect
pH: electrode slope, buffers, samples, reference element drift, and
temperature sensor errors.
Electrode Slope Changes
The electrode slope will change with variations in temperature.
Slope changes may be compensated manually, automatically with
an automatic temperature compensation (ATC) probe or with LogR
technology using a LogR enabled meter and any Orion electrode.
Thermo Scientific Orion pH meters calculate the slope based on the
measured temperature and automatically adjust the pH value based on
the temperature.
