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ROSS Ultra, ROSS Ultra Triode and ROSS pH Electrodes

Thermo Scientific Orion

• 

Flat surface ROSS pH electrodes may be used on any 

moist surface or in liquids. 

Using a Flat Surface Electrode

Electrode Calibration

General Calibration Procedure 

For detailed instructions on pH calibration, manual pH calibration and 

temperature compensation, consult your meter user guide. When 

using ROSS electrodes with a 

V

ersa

 s

tar

 pH/LogR meter, refer to the 

V

ersa

 s

tar

 pH/LogR meter user guide for instructions on temperature 

calibration and LogR temperature compensated pH measurements.

Note:

 If the meter is set to automatically recognize the pH buffer at the 

measured temperature and an ATC probe is used, you do not need to 

manually adjust the pH values of the buffers.

One Buffer Calibration

1.   Choose a buffer near expected sample pH.
2.   The buffer should be at same temperature as the sample. If the 

buffer and samples are at varying temperatures, temperature 

compensation is recommended.

3.   Prepare the meter according to the meter user guide.
4.   Rinse the electrode first with distilled water and then with the buffer 

being used for calibration.

Filling hole should

be uncovered

Fresh pH buffer

Stir all samples

and buffers

Filling solution level 
must be higher than 
sample level

Reference junction
must be immersed

Place insulation 
between stirrer 
and beaker

Measuring Hints

Summary of Contents for Orion ROSS

Page 1: ...ROSS Ultra ROSS Ultra Triode and ROSS pH Electrodes 263745 001 Revision B November 2014 Thermo Scientific Orion U ser Manual...

Page 2: ......

Page 3: ...ode Calibration 10 General Calibration Procedure 10 One Buffer Calibration 10 Two Buffer Calibration 11 pH Measurements 12 Electrode Storage 12 Electrode Maintenance 12 Electrode Cleaning Procedures 1...

Page 4: ...s are three to five times more precise than those obtained with conventional electrodes Over the lifetime of the electrode ROSS reference potential drifts less than an average 0 12mV per day which cor...

Page 5: ...ientific Orion Star A200 series Thermo Scientific Orion Star A100 series or Thermo Scientific Orion Dual Star meter with pH measurement capability such as the Versa Star benchtop meter with pH module...

Page 6: ...3 The electrode may arrive with the chamber partially filled with electrolyte solution Please fill the chamber with electrolyte solution before use Uncover the fill hole by removing the tape and add...

Page 7: ...an adequate flow rate the level of fill solution must always be above the reference junction and at least one inch above the sample level The fill hole should remain open whenever the electrode is in...

Page 8: ...on aqueous solutions and solutions that contain organic solvents A minimum of 20 water must be present in the sample for the best measurement results If there is a great deal of drift when using the R...

Page 9: ...a uniform rate Place a piece of insulating material such as polystyrene foam or cardboard between the magnetic stir plate and beaker to prevent measurement errors from the transfer of heat to the sam...

Page 10: ...the meter is set to automatically recognize the pH buffer at the measured temperature and an ATC probe is used you do not need to manually adjust the pH values of the buffers One Buffer Calibration 1...

Page 11: ...rode into the first buffer When the reading is stable set the meter to the pH value of the buffer at the measured temperature Refer to the meter user guide for a detailed procedure Table 1 provides pH...

Page 12: ...flush it with fresh ROSS fill solution and refill the chamber with fresh ROSS fill solution Electrode Cleaning Procedures General Cleaning We offer a variety of Orion pH cleaning solutions for your c...

Page 13: ...n Cat No 910001 for at least one hour Cleaning Solutions Cat No 900021 WA pH cleaning solution A for removing protein contaminants Cat No 900022 WA pH cleaning solution B for removing bacterial contam...

Page 14: ...C probe are placed into a sample that varies significantly in temperature the readings can drift for two reasons First the temperature response of the electrode and temperature probe may not be simila...

Page 15: ...m precision a three point temperature calibration is recommended Do not perform a one point temperature calibration if measured solutions will be below 20 C The following tables illustrate the expecte...

Page 16: ...s and at higher temperatures Because the pH membrane is composed of special low sodium error glass error due to sodium is negligible when measuring at pH values less than 12 When measuring at pH value...

Page 17: ...lectrode into fresh pH 4 buffer When the reading is stable record the mV value of the pH 4 buffer The mV value should be in the range of 150 to 210 mV 4 Calculate the absolute mV difference between th...

Page 18: ...Mercury Hg Cadmium Cd Sensor body with cable assembly X 0 0 Plastic hardware 0 0 0 Part Name Toxic or Hazardous Substances and Elements Hexavalent Chromium Cr VI Polybrominated biphenyls PBB Polybromi...

Page 19: ...combination pH electrode with epoxy body and semi micro tip waterproof BNC connector 8135BNUWP ROSS Ultra combination pH electrode with epoxy body and flat surface tip waterproof BNC connector 8156BN...

Page 20: ...ctrode with epoxy body waterproof BNC connector 8165DN ROSS Sure Flow combination pH electrode with epoxy body E DIN waterproof connector 8172BNWP ROSS Sure Flow combination pH electrode with glass bo...

Page 21: ...scientific com water for additional accessories buffers and buffer sizes Specifications pH Range 0 to 14 pH pH Precision 0 01 pH Temperature Range 0 to 100 C ROSS Ultra Triode Temperature Accuracy 1 C...

Page 22: ...8102BN 8104BN 8135BN 815600 8162SC 8165BNWP 8172BNWP 8302BNURCA 8107BNURCA 8157BNURCA 120 mm length 12 mm diameter 8103BNUWP 8103BN 8103SC 165 mm length 95 mm tip length 6 mm tip diameter 8115BNUWP 8...

Page 23: ......

Page 24: ...moscientific com water Netherlands Tel 31 020 4936270 info water uk thermo com China Tel 86 21 68654588 wai asia thermofisher com India Tel 91 22 4157 8800 wai asia thermofisher com Singapore Tel 65 6...

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